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- rabbit polyclonal
reactivity: human, mouse, rat, dog
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section
citations: 6
Immunoprecipitation: NFAT5 Antibody [NB120-3446] - Analysis of NFAT5 was performed on U2OS cells. The antigen:antibody complex was formed by incubating 500ug whole cell lysate with 3ug of rabbit polyclonal antibody recognizing NFAT5 overnight on a rocking platform at 4C. The immune-complex was captured on 50ul Protein A/G Plus Agarose. Captured immune-complexes were washed and proteins eluted with 5X Reducing Sample Loading Dye. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% Milk/TBS-0.1%Tween for at least 1 hour. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura.
Immunocytochemistry/Immunofluorescence: NFAT5 Antibody [NB120-3446] - Analysis of NFAT5 using anti-NFAT5 polyclonal antibody (shown in green) in HeLa cells.
Western Blot: NFAT5 Antibody [NB120-3446] - Analysis of NFAT5 was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a rabbit polyclonal antibody recognizing NFAT5 at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura.
quantity: 50 ug
price: 349 USD
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gene information - dog NFAT5
- synonym:nuclear factor of activated T-cells 5; nuclear factor of activated T-cells 5, tonicity-responsive
- description:nuclear factor of activated T cells 5
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product type
- NFAT5 antibody
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