dog HIF1A antibody | 9 (3 knockout-validated) products in Validated Antibody Database; 13 cited in the literature; 23 total from 7 suppliers

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HIF-1 alpha Antibody (H1alpha67)
Novus Biologicals
catalog: NB100-105

total number of products: 7

mouse monoclonal (H1alpha67)
reactivity: frogs and toads, primates, human, mouse, rat, dog, cow, pig, sheep, rabbit
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, proximity ligation assay, western blot knockout validation
citations: 786

Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Staining of HIF1 alpha in human kidney. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining.

Flow Cytometry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-105. Staining of HIF-1 alpha in H929 cells using HIF-1 alpha antibody. Image from verified customer review.

Flow Cytometry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-105. Staining of HIF-1 alpha in multiple myeloma cells: H929 cells (0.5x10^6) were stained with Alexa Fluor 488 (R) conjugated HIF-1a antibody (NB100-105AF488). Image courtesy of Dr. Barbara Muz at Washington University in St. Louis School of Medicine.
quantity: 0.1 ml (also 0.025 ml)
price: 349 USD
to the supplier
HIF-1 alpha Antibody
Novus Biologicals
catalog: NB100-479

total number of products: 7

rabbit polyclonal
reactivity: goat, hamsters, human, mouse, rat, dog, rabbit
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 313

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-479] - Analysis in paraffin section of mouse skin wound using NB100-479 at 1:6,000. Image courtesy of product review by Ian Darby of RMIT University.

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-479] - Analysis in human kidney tumor.

Western Blot: HIF-1 alpha Antibody [NB100-479] - Analysis in a hypoxic sample. Lane 1: CoCl2 treated Cos-7 nuclear extract (50 ug, hypoxic). Lane 2: Untreated Cos-7 nuclear extract (50 ug, normoxic). 10 second ECL exposure.
quantity: 0.1 ml (also 0.025 ml)
price: 349 USD
to the supplier
HIF-1 alpha Antibody
Novus Biologicals
catalog: NB100-449

total number of products: 7

rabbit polyclonal
reactivity: human, mouse, rat, dog, chicken
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 208

Proximity Ligation Assay: HIF-1 alpha Antibody [NB100-449] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM2 (NB100-244) and rabbit anti-human HIF1-alpha (NB100-449). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.

Western Blot: HIF-1 alpha Antibody [NB100-449] - Analysis of HIF-1 alpha in human myeloma cell lysate using anti-HIF-1 alpha. Cells were untreated or treated with IGF-1, IL-6 or CoCl2. Image from verified customer review.

Western Blot: HIF-1 alpha Antibody [NB100-449] - Detection of Human HIF1 alpha by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells that were either treated with cobalt chloride (+; 200 mcM) or mock treated (-). Antibodies: Affinity purified rabbit anti-HIF1 alpha antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. HIF1 alpha was also immunoprecipitated by a previous lot of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
quantity: 0.1 ml
price: 399 USD
to the supplier
HIF1A Monoclonal Antibody (H1alpha67)
Invitrogen
catalog: MA1-16511

total number of products: 9

mouse monoclonal (H1alpha67)
reactivity: human, mouse, rat, dog, cow, pig, sheep, rabbit
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 50

Immunohistochemical staining of HIF-1 alpha in human kidney using MA1-16511.

Immunoprecipitation of HIF-1 alpha; Heavy and light chains are also detected.

Western Blot detection of HIF-1 alpha in cobalt chloride treated and untreated COS-7 nuclear extracts.
quantity: 100µL
price: US 390
to the supplier
HIF1A Monoclonal Antibody (H1alpha67), Biotin
Invitrogen
catalog: MA1-46457

total number of products: 9

mouse monoclonal (H1alpha67)
reactivity: human, mouse, rat, dog, cow, pig, sheep, rabbit
conjugate: biotin
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 39

Immunoprecipitation of HIF-1alpha using MA1-46457; Heavy and light chains are also detected.

Immunohistochemical staining of HIF-1 alpha in human kidney using MA1-46457.

On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted with RIPA buffer fortified with protease. Upon quantification, 100ug of protein was fractionated on 7% polyacralymide gel. -Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution. The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution.
quantity: 100µL
price: US 345
to the supplier
HIF1A Polyclonal Antibody
Invitrogen
catalog: PA3-16521

total number of products: 9

rabbit polyclonal
reactivity: Domestic guinea pig, human, mouse, rat, dog, common platanna
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 3

Immunohistochemical staining of human kidney, renal tubular epithelium in Cortex using PA3-16521.

Western Blot analysis of PA3-16521, anti-HIF-1 alpha, on normoxic and hypoxic nuclear rat cell lysates.
quantity: 100µL
price: US 390
to the supplier
Anti-HIF1a
SICGEN
catalog: AB0112-200

goat polyclonal
reactivity: human, mouse, rat, dog, cow
application: western blot
citations: 3

quantity: 600 µg
price: 320 euros
to the supplier
Anti-HIF1a
SICGEN
catalog: AB0070-100

goat polyclonal
reactivity: human, mouse, rat, dog, cow
application: western blot
citations: 1

quantity: 300 µg
price: 320 euros
to the supplier
HIF-1α monoclonal antibody (mgc3)
Enzo Life Sciences
catalog: ALX-804-216-R100

mouse monoclonal (mgc3)
reactivity: human, mouse, rat, dog, cow
application: western blot, immunocytochemistry, immunoprecipitation
citations: 3

Figure B: Electrophoretic mobility shift assay (EMSA) using MAb to Hypoxia-inducible Factor 1alpha (mgc3) (Prod. No. ALX-804-216).Method: Nuclear extracts (5µg) from normoxic and hypoxic human HeLa and mouse wild-type (Hepa1) and ARNT-mutant (Hepa1C4) hepatoma cells were analyzed by EMSA. MAb mgc3 supershifted the HIF-1 heterodimer in HeLa and Hepa1 cells. No HIF DNA-binding activity was detected in ARNT-mutant Hepa1C4 cells. In contrast to Western blot analysis, mgc3 was able to detect also non-human HIF-1alpha in EMSA [1,2].

Figure A: Western blot using MAb to Hypoxia-inducible Factor 1alpha (mgc3) (Prod. No. ALX-804-216).Method: Nuclear extracts (25µg) from normoxic (20% O2) and hypoxic (1% O2) human HeLa and mouse Hepa1 cells were subjected to SDS-PAGE and HIF-1alpha was detected in Western blots using MAb mgc3 (1:1'000). Note that mgc3 detects denatured HIF-1alpha only in human cells in Western blot analysis [1].
quantity: 100 µl
price: 607 USD
to the supplier
HIF-1-alpha hydroxy P564 Antibody
Rockland Immunochemicals
catalog: 100-401-A25

rabbit polyclonal
reactivity: human, mouse, rat, dog, cow
application: western blot, ELISA, immunohistochemistry

Western blot using Rockland's anti-HIF-1alphaHYDROXYP564 antibody shows detection (left panel) of hydroxylated HIF-1alpha in nuclear extracts of A549 cells treated with MG132 (a proteosome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa in size corresponding to HIF1-????The primary antibody was used at a 1:1,000 dilution in 2% BLOTTO. Personal Communication, L. Neckers and O. Aprelikova, NCI, Bethesda, MD.
quantity: 100 µL
price: 369 USD
to the supplier
HIF1 alpha antibody
Biorbyt
catalog: orb216126

rabbit polyclonal
reactivity: human, dog
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Raji (Lane 1), A431 (Lane 2), HeLa (Lane 3) whole cell lysates using HIF1 alpha antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded human brain tissue using HIF1 alpha antibody (dilution at 1:200)
quantity: 30 ul (Trial size), 100 ul, 200 ul
price: 106.0, 169.0, 243.0 USD
to the supplier
HIF1A monoclonal antibody, clone ESEE122
Abnova
catalog: MAB2342

mouse monoclonal (ESEE122)
reactivity: human, mouse, rat, dog, cow
application: immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

quantity: 100 uL
price:
to the supplier
HIF1A polyclonal antibody
Abnova
catalog: PAB9946

rabbit polyclonal
reactivity: human, mouse, rat, dog, cow
application: western blot, ELISA, immunohistochemistry

quantity: 100 uL
price:
to the supplier