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- mouse monoclonal (TE111.5D11)
reactivity: Chinese hamster, human, mouse, rat, dog, chicken, cow, pig, sheep, rabbit
application: western blot, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments
citations: 68 - rabbit monoclonal (IE-5)
reactivity: human, mouse, rat, dog
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, chromatin immunoprecipitation
Western blot analysis of ER alpha expression in (1) MCF7 cell lysate; (2)T47-D cell lysate (M00057-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESR1 monoclonal antibody (Catalog # M00057-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESR1
Immunohistochemical analysis of paraffin-embedded human cervix carcinoma, using ER alpha Antibody(M00057-2). ESR1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ESR1 Antibody (M00057-2)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IF analysis of immunocytochemical section of MCF7 cells using anti- ER alpha antibody (M00057-2) . ER alpha was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti- ER alpha Antibody (M00057-2) overnight at 4 C. DyLight®488 Conjugated Goat AntiRabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used
quantity: 100 ug/vial
price: 299 USD
to the supplier - rabbit polyclonal
reactivity: human, dog, cow
application: western blot, immunohistochemistry - paraffin section
citations: 1
gene information - dog ESR1
- synonym:ERALPHA; estrogen receptor
- description:estrogen receptor 1
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product type
- ESR1 antibody
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