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- rabbit polyclonal
reactivity: human, mouse, rat, dog
application: western blot, immunocytochemistry, chromatin immunoprecipitation
citations: 2
Figure: WB using PAb to mTOR (human FRB Domain) (Prod. No. ALX-215-065) on total yeast extracts derived from cells expressing FRB-tagged genes coding for three different transcription factors. Separation SDS-PAGE.
Figure 2: Western Blot of mTOR (human FRB Domain), pAb (Prod. No. ALX-215-065) with Hela cells.Method:Two ml of an exponential HeLa suspension culture (2-4x105 cells) was washed twice with PBS quickly without resuspention of the pellet by spinning in a Eppendorf tube and finally with 25% TCA. TCA was carefully removed and pellet respuspended 100µl FSB + ßME (final sample buffer) and the pH adjusted with about a few µl of 1M Tris base (‰ˆ10 µl). The sample was heated at 100°C for 5 min. and sonicated. SDS PAGE was at 7.5%, 20 & 25 µl loaded in lane 1 and 2, respectively. The TCA step is performed to prevent proteolytic breakdown of the very large mTOR.
quantity: 1 vial
price: 434 USD
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gene information - dog COL4A1
- synonym:collagen alpha-1(IV) chain
- description:collagen type IV alpha 1 chain
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product type
- COL4A1 antibody
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