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- mouse monoclonal (V63)
reactivity: human, rat, dog
application: western blot, immunohistochemistry
citations: 1 - rabbit polyclonal
reactivity: human, rat, dog, chimpanzee
application: western blot, ELISA, immunoprecipitation - rabbit polyclonal
reactivity: human, mouse, rat, dog, chimpanzee
application: western blot, ELISA, immunoprecipitation
Western blot using Rockland's Affinity Purified anti-Cyclin B 1 pS126 antibody shows detection of a band ~48 kDa corresponding to phosphorylated human Cyclin B 1 (arrowheads) in various whole cell lysates. Lysates tested were lane 1 - Hela (cervical carcinoma), lane 2 - H23 (lung carcinoma), lane 3 - Hep3b (Hepatocarcinoma), lane 4 - T98G (Glioblastoma) and lane 5 - Daudi (B cell lymphoblast). Each lane contains approximately 50 µg of lysates, separated by 12% SDS-PAGE using a 5% stack run at 100 volts until the dye front cleared the bottom of the gel. Transfer occurred overnight at 4° C at 15 mAmps. The membrane was blocked with 5% non-fat dry milk in TTBS for 1 h at room temperature followed by addition of a 1:100 dilution of the antibody allowed to react for 2h at room temperature. After washes with TTBS a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG [H&L] MX (611-103-122) was added for 1 h at room temperature. After additional washes the membrane was incubated with ECL mix 1:1 for ~3 min. Excess detection solution was drained off and the membrane was exposed to Kodak film X-omat blue XB-1 for about 20 sec. Other detection systems will yield similar results. Personnel Communication, Luca Cote, Temple U.
quantity: 100 µg
price: 369 USD
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gene information - dog CCNB1
- synonym:G2/mitotic-specific cyclin-B1
- description:cyclin B1
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- CCNB1 antibody
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