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- mouse monoclonal (45)
reactivity: human, mouse, rat, dog
application: western blot, immunocytochemistry, immunoprecipitation
citations: 1 - rabbit polyclonal
reactivity: human, mouse, rat, dog
application: western blot, ELISA
Western blot using Rockland's Affinity Purified anti-CaM Kinase II antibody shows detection of a band ~54 kDa corresponding to human alpha CaM Kinase II (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). Approximately 35 ?g of a mouse brain whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. CaM Kinase II was similarly detected on lysates from rat brain (not shown). After blocking the membrane was probed with the primary antibody diluted to 1:1,500 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
quantity: 100 µg
price: 369 USD
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gene information - dog CAMK2A
- synonym:calcium/calmodulin-dependent protein kinase type II subunit alpha; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
- description:calcium/calmodulin dependent protein kinase II alpha
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product type
- CAMK2A antibody
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