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- rabbit polyclonal
reactivity: human, mouse, rat, dog, chicken, cow
application: western blot, immunohistochemistry, immunocytochemistry
Western blot analysis of BAF53A expression in K562 (A), Raw264.7 (B), PC12 (C), H9C2 (D) whole cell lysates.
Immunohistochemical analysis of BAF53A staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of BAF53A staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
quantity: 0.03 mL
price: 155 USD
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gene information - dog ACTL6A
- synonym:actin-like protein 6A
- description:actin like 6A
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- ACTL6A antibody
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