Your Filters
product
- goat polyclonal
reactivity: human, mouse, rat, dog, chicken, cow, chimpanzee
application: western blot, ELISA - goat polyclonal
reactivity: human, mouse, dog, cow
application: western blot, ELISA - goat polyclonal
reactivity: human, mouse, rat, dog, chicken, cow, chimpanzee
application: western blot, ELISA
Immunofluorescence Microscopy of Goat anti-RING1B antibody. Tissue: human HeLa cells. Fixation: methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25°C. Antigen retrieval: not required. Primary antibody: RING1B antibody at 1:300 for 20 minutes at 25°C. Secondary antibody: Alexa Fluor®488-conjugated Donkey anti-goat IgG secondary antibody at 1:500 for 45 min at RT. Localization: RING1B is nuclear and occasionally cytoplasmic. Staining: RING1B (RNF2) as green signal, Tubulin cytoplasm staining red, and DAPI (blue) nuclear counterstain.
Western blot using Rockland's Affinity Purified anti-RING1B antibody shows detection of a 38 kDa band corresponding to human RING1B in 3T3 (lane 1), U937 (lane 2), Jurkat (lane 3), mouse brain (lane 4) and CHO-K1 (lane 5) cell lysates. Approximately 20 µg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-RING1B antibody incubated at room temper-ature. Signal was detected using standard techniques.
quantity: 100 µg
price: 369 USD
to the supplier - rabbit polyclonal
reactivity: Domestic guinea pig, human, mouse, rat, dog, cow, zebrafish , rabbit, equine
application: western blot, immunohistochemistry - rabbit polyclonal
reactivity: human, mouse, dog, chicken, cow
application: western blot
citations: 1
gene information - cow RNF2
- synonym:E3 ubiquitin-protein ligase RING2
- description:ring finger protein 2
do you know?
- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
product type
- RNF2 antibody
species
questions and comments