cow HIF1A antibody | 12 (2 knockout-validated) products in Validated Antibody Database; 22 cited in the literature; 40 total from 12 suppliers

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HIF-1 alpha Antibody (H1alpha67)
Novus Biologicals
catalog: NB100-105

total number of products: 4

mouse monoclonal (H1alpha67)
reactivity: primates, frogs and toads, human, mouse, rat, dog, cow, pig, sheep, rabbit
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, proximity ligation assay, western blot knockout validation
citations: 786

Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Staining of HIF1 alpha in human kidney. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining.

Flow Cytometry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-105. Staining of HIF-1 alpha in H929 cells using HIF-1 alpha antibody. Image from verified customer review.

Flow Cytometry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-105. Staining of HIF-1 alpha in multiple myeloma cells: H929 cells (0.5x10^6) were stained with Alexa Fluor 488 (R) conjugated HIF-1a antibody (NB100-105AF488). Image courtesy of Dr. Barbara Muz at Washington University in St. Louis School of Medicine.
quantity: 0.1 ml (also 0.025 ml)
price: 349 USD
to the supplier
HIF-1 alpha Antibody (H1alpha67)
Novus Biologicals
catalog: NB100-123

total number of products: 4

mouse monoclonal (H1alpha67)
reactivity: birds, human, mouse, rat, dog, cow, pig, rabbit
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section, western blot knockout validation
citations: 150

Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Staining of Biotin conjugated HIF-1 alpha in human kidney.

Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Analysis using the Biotin conjugate of NB100-123. On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted with RIPA buffer fortified with protease. Upon quantification, 100ug of protein was fractionated on 7% polyacralymide gel. Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution (NB100-123). The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution. Photo courtesy of Dr. Gregg Semenza, Johns Hopkins University.

Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - HIF staining in pig endothelial cells under hipoxia condition. Image from verified customer review.
quantity: 0.1 ml (also 0.025 ml)
price: 349 USD
to the supplier
HIF-1 alpha Antibody (ESEE122)
Novus Biologicals
catalog: NB100-131

total number of products: 4

mouse monoclonal (ESEE122)
reactivity: human, mouse, rat, dog, cow
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 74

Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line, using NB100-131. Photos courtesy of Susan Alexander and Hattie Gresham, PhD.

Simple Western: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Simple Western lane view shows a specific band for HIF-1 alpha in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Negative control stain of human placenta (from sea level) using mouse IgG at 1:100. 4uM paraffin-embedded section.
quantity: 0.1 ml (also 0.025 ml)
price: 349 USD
to the supplier
HIF1A Monoclonal Antibody (H1alpha67)
Invitrogen
catalog: MA1-16504

total number of products: 17

mouse monoclonal (H1alpha67)
reactivity: human, mouse, rat, cow, pig, sheep, rabbit
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, EMSA, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 54

Western Blot analysis of 50ug cobalt chloride (CoCl2) induced COS-7 nuclear extracts.

Immunohistochemical staining of HIF1 alpha in human kidney. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining.
quantity: 100µL
price: US 390
to the supplier
HIF1A Monoclonal Antibody (H1alpha67)
Invitrogen
catalog: MA1-16511

total number of products: 17

mouse monoclonal (H1alpha67)
reactivity: human, mouse, rat, dog, cow, pig, sheep, rabbit
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 50

Immunohistochemical staining of HIF-1 alpha in human kidney using MA1-16511.

Immunoprecipitation of HIF-1 alpha; Heavy and light chains are also detected.

Western Blot detection of HIF-1 alpha in cobalt chloride treated and untreated COS-7 nuclear extracts.
quantity: 100µL
price: US 390
to the supplier
HIF1A Monoclonal Antibody (H1alpha67), Biotin
Invitrogen
catalog: MA1-46457

total number of products: 17

mouse monoclonal (H1alpha67)
reactivity: human, mouse, rat, dog, cow, pig, sheep, rabbit
conjugate: biotin
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 39

Immunoprecipitation of HIF-1alpha using MA1-46457; Heavy and light chains are also detected.

Immunohistochemical staining of HIF-1 alpha in human kidney using MA1-46457.

On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted with RIPA buffer fortified with protease. Upon quantification, 100ug of protein was fractionated on 7% polyacralymide gel. -Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution. The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution.
quantity: 100µL
price: US 345
to the supplier
HIF1 alpha antibody [mgc3]
Abcam
catalog: ab16066

mouse monoclonal (mgc3)
reactivity: human, mouse, cow
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, EMSA, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 26

quantity: 100 ul
price:
to the supplier
Mouse monoclonal [ESEE122] to HIF-1-alpha
Abcam
catalog: ab8366

mouse monoclonal (ESEE122)
reactivity: human, mouse, rat, cow
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 17

quantity:
price:
to the supplier
Rabbit polyclonal to HIF-1-alpha
Abcam
catalog: ab114977

rabbit polyclonal
reactivity: human, mouse, rat, chicken, cow, pig, zebrafish , rabbit
citations: 1

quantity:
price:
to the supplier
Anti-HIF1a
SICGEN
catalog: AB0112-200

goat polyclonal
reactivity: human, mouse, rat, dog, cow
application: western blot
citations: 2

quantity: 600 µg
price: 320 euros
to the supplier
Anti-HIF1a
SICGEN
catalog: AB0070-100

goat polyclonal
reactivity: human, mouse, rat, dog, cow
application: western blot
citations: 1

quantity: 300 µg
price: 320 euros
to the supplier
HIF-1α monoclonal antibody (mgc3)
Enzo Life Sciences
catalog: ALX-804-216-R100

mouse monoclonal (mgc3)
reactivity: human, mouse, rat, dog, cow
application: western blot, immunocytochemistry, immunoprecipitation
citations: 3

Figure B: Electrophoretic mobility shift assay (EMSA) using MAb to Hypoxia-inducible Factor 1alpha (mgc3) (Prod. No. ALX-804-216).Method: Nuclear extracts (5µg) from normoxic and hypoxic human HeLa and mouse wild-type (Hepa1) and ARNT-mutant (Hepa1C4) hepatoma cells were analyzed by EMSA. MAb mgc3 supershifted the HIF-1 heterodimer in HeLa and Hepa1 cells. No HIF DNA-binding activity was detected in ARNT-mutant Hepa1C4 cells. In contrast to Western blot analysis, mgc3 was able to detect also non-human HIF-1alpha in EMSA [1,2].

Figure A: Western blot using MAb to Hypoxia-inducible Factor 1alpha (mgc3) (Prod. No. ALX-804-216).Method: Nuclear extracts (25µg) from normoxic (20% O2) and hypoxic (1% O2) human HeLa and mouse Hepa1 cells were subjected to SDS-PAGE and HIF-1alpha was detected in Western blots using MAb mgc3 (1:1'000). Note that mgc3 detects denatured HIF-1alpha only in human cells in Western blot analysis [1].
quantity: 100 µl
price: 607 USD
to the supplier
HIF1 alpha Antibody
StressMarq Biosciences
catalog: SMC-184D

mouse monoclonal (ESEE122)
reactivity: human, mouse, rat, cow
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
citations: 1

quantity: 100 µg
price: 263.00 USD
to the supplier
HIF-1 alpha antibody (mAb)
Active Motif
catalog: 61275

mouse monoclonal (ESEE122)
reactivity: human, mouse, rat, cow
application: western blot, immunohistochemistry
citations: 1

quantity: 100 ug
price: 350 USD
to the supplier
HIF1A / HIF1 Alpha Antibody (aa45-180) LS-C294554
LifeSpan Biosciences
catalog: LS-C294554

rabbit polyclonal
reactivity: human, cow
application: western blot

Western blot of recombinant HIF1A / HIF1 Alpha. This image was taken for the unconjugated form of this product. Other forms have not been tested.
quantity:
price:
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HIF1A / HIF1 Alpha Antibody (aa45-180, Biotin) LS-C300269
LifeSpan Biosciences
catalog: LS-C300269

rabbit polyclonal
reactivity: human, cow
conjugate: biotin
application: western blot

Western blot of recombinant HIF1A / HIF1 Alpha. This image was taken for the unconjugated form of this product. Other forms have not been tested.
quantity:
price:
to the supplier
HIF1A / HIF1 Alpha Antibody (aa220-349) LS-C374087
LifeSpan Biosciences
catalog: LS-C374087

rabbit polyclonal
reactivity: human, cow
application: western blot

Western blot of HIF1A / HIF1 Alpha antibody.
quantity:
price:
to the supplier
HIF-1-alpha hydroxy P564 Antibody
Rockland Immunochemicals
catalog: 100-401-A25

rabbit polyclonal
reactivity: human, mouse, rat, dog, cow
application: western blot, ELISA, immunohistochemistry

Western blot using Rockland's anti-HIF-1alphaHYDROXYP564 antibody shows detection (left panel) of hydroxylated HIF-1alpha in nuclear extracts of A549 cells treated with MG132 (a proteosome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa in size corresponding to HIF1-????The primary antibody was used at a 1:1,000 dilution in 2% BLOTTO. Personal Communication, L. Neckers and O. Aprelikova, NCI, Bethesda, MD.
quantity: 100 µL
price: 369 USD
to the supplier
HIF-1-alpha Antibody
Rockland Immunochemicals
catalog: 200-301-F45

mouse monoclonal (ESEE122)
reactivity: human, mouse, rat, cow
application: western blot, immunohistochemistry

Western Blot of mouse anti-HIF1 alpha antibody. Lane 1: HeLa cell lysates. Lane 2: none. Load: 35 µg per lane. Primary antibody: HIF1 alpha antibody at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 93.5 kDa, ~106 kDa for HIF1?. Other band(s): none.
quantity: 100 µg
price: 369 USD
to the supplier
HIF1A monoclonal antibody, clone ESEE122
Abnova
catalog: MAB2342

total number of products: 4

mouse monoclonal (ESEE122)
reactivity: human, mouse, rat, dog, cow
application: immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

quantity: 100 uL
price:
to the supplier
HIF1A polyclonal antibody
Abnova
catalog: PAB12419

total number of products: 4

rabbit polyclonal
reactivity: human, mouse, cow
application: western blot, immunohistochemistry

quantity: 100 uL
price:
to the supplier
HIF1A polyclonal antibody
Abnova
catalog: PAB12420

total number of products: 4

rabbit polyclonal
reactivity: human, mouse, rat, chicken, cow
application: western blot

quantity: 100 uL
price:
to the supplier
HIF-1 alpha Antibody [ESEE122]: Fluorescein
ImmuQuest
catalog: IQ607FITC

mouse monoclonal ('ESEE122)
reactivity: human, mouse, rat, cow
conjugate: FITC
application: immunocytochemistry

quantity: 50 ul
price: 380 USD
to the supplier
HIF-1-alpha Antibody, hydroxyproline (Pro402)
EMD Millipore
catalog: 07-1585

rabbit polyclonal
reactivity: human, mouse, cow, zebrafish
application: western blot
citations: 1

quantity: 100 μg
price:
to the supplier