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- mouse monoclonal (JA9)
reactivity: human, mouse, rat, cow, pig, rabbit
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 18
Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing PMCA4 ATPase (MA1-914) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
quantity: 100µg
price: 381 USD
to the supplier - mouse monoclonal (JA9)
reactivity: human, mouse, rat, cow
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry knockout validation
citations: 10 - mouse monoclonal (JA9)
reactivity: human, mouse, rat, cow
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 7 
Immunocytochemistry/Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569] - Immunolocalization of (A) PMCA4 (green), (B) CaSRO (red), and (C) Merged image.
Immunocytochemistry/Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569] - Immunolocalization of (A) PMCA4 (green), (B) CaSRO (red), and (C) Merged image.
Immunohistochemistry-Paraffin: PMCA4 Antibody (JA9) [NB300-569] - Normal biopsies of deparaffinized Human brain tissue.
quantity: 100 ug
price: 329 USD
to the supplier
gene information - cow ATP2B4
- synonym:PMCA4; plasma membrane calcium-transporting ATPase 4; ATPase, Ca++ transporting, plasma membrane 4 ATP synthase, H+ transporting, mitochondrial F1
- description:ATPase plasma membrane Ca2+ transporting 4
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product type
- ATP2B4 antibody
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