Your Filters
product
- rabbit polyclonal
reactivity: human, mouse, rat, dog, cow
application: western blot, ELISA, immunoprecipitation
Western blot using Rockland's Affinity Purified anti-APC1 pS355 antibody shows detection of a band ~215 kDa corresponding to phosphorylated human APC1 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with thymidine to synchronize cells at the G1/S boundary. Lane 3 shows lysate from cells treated with nocodazole to synchronize cells at the M phase. Phosphorylated APC1 is mostly present only in cell preparations arrested at cell division. Each lane contains approximately 30 ?g of HeLa S3 whole cell lysates separated by 12.5% SDS-PAGE followed by transfer to nitrocellulose. After blocking with 5% non-fat dry milk in TTBS, the membrane was probed with the primary antibody diluted to 1:500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of HRP Gt-a-Rabbit IgG [H&L] MX (611-103-122) for 45 min at room temperature. ECL reagent was used for detection. Other detection systems will yield similar results. Data contributed by Bing Li, UT Southwestern.
quantity: 100 µg
price: 369 USD
to the supplier - rabbit polyclonal
reactivity: human, mouse, rat, dog, chicken, cow
application: western blot, ELISA, immunoprecipitation - rabbit polyclonal
reactivity: human, mouse, rat, dog, cow
application: western blot, ELISA, immunohistochemistry - paraffin section
gene information - cow ANAPC1
- synonym:anaphase-promoting complex subunit 1
- description:anaphase promoting complex subunit 1
do you know?
- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
product type
- ANAPC1 antibody
species
linkout
questions and comments