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- domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs, chimpanzee
application: western blot, ELISA
citations: 1
Western blot analysis is shown using Rockland's Affinity Purified anti-UBC12 antibody to detect Human UBC12 in various preparations. This western blot shows reactivity with purified human UBC12 protein (lane 1), NIH-3T3 cells over expressing UBC12 by infection (lane 2) and endogenous UBC12 in NIH 3T3 cells (lane 3). Peptide competition (lane 4) blocks specific reactivity of the antibody with purified UBC12 protein. Comparison to a molecular weight marker (not shown) indicates a single band of ~21.0 kDa corresponding to the expected molecular weight for the protein. The blot was blocked with 5% non-fat dry milk in TBS supplemented with 0.1% Tween-20 at 4° C overnight. After washes the blot was incubated with a 1:1,000 dilution of the antibody at room temperature for 2 h in TBS-Tween. Washes consisted of 3 changes of TBS-Tween buffer for 15 min each. Detection occurred using HRP anti-Rabbit IgG diluted 1:2,000 and signal processing by chemiluminescence reagent with a 10-sec exposure time. Other detection systems will yield similar results. Personal communication Martine Roussel.
quantity: 100 µg
price: 369.00 USD
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gene information - chimpanzee UBE2M
- description:ubiquitin conjugating enzyme E2 M
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- UBE2M antibody
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