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- domestic goat polyclonal
reactivity: human, mouse, rat, dogs, chicken, bovine, chimpanzee
application: western blot, ELISA
Immunofluorescence Microscopy of Goat anti-RING1B antibody. Tissue: human HeLa cells. Fixation: methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25°C. Antigen retrieval: not required. Primary antibody: RING1B antibody at 1:300 for 20 minutes at 25°C. Secondary antibody: Alexa Fluor®488-conjugated Donkey anti-goat IgG secondary antibody at 1:500 for 45 min at RT. Localization: RING1B is nuclear and occasionally cytoplasmic. Staining: RING1B (RNF2) as green signal, Tubulin cytoplasm staining red, and DAPI (blue) nuclear counterstain.
Western blot using Rockland's Affinity Purified anti-RING1B antibody shows detection of a 38 kDa band corresponding to human RING1B in 3T3 (lane 1), U937 (lane 2), Jurkat (lane 3), mouse brain (lane 4) and CHO-K1 (lane 5) cell lysates. Approximately 20 µg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-RING1B antibody incubated at room temper-ature. Signal was detected using standard techniques.
quantity: 100 µg
price: 369 USD
to the supplier - domestic goat polyclonal
reactivity: human, mouse, rat, dogs, chicken, bovine, chimpanzee
application: western blot, ELISA
gene information - chimpanzee RNF2
- description:ring finger protein 2
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product type
- RNF2 antibody
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