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- domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs, chicken, chimpanzee
application: western blot, ELISA, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs, chicken, chimpanzee, swine, zebrafish , Xenopus laevis
application: western blot, immunohistochemistry, enzyme immunoassay
Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates.,.transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-.,.Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDSPAGE.,.and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous.,.plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these.,.mutants. Personal communication Hai Jiang, Northwestern Univ.
quantity: 0.1 mg
price: 489.5 USD
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gene information - chimpanzee PLK1
- description:polo like kinase 1
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- PLK1 antibody
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