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- mouse monoclonal (14E5.16C8.25F6)
reactivity: human, mouse, rat, chimpanzee
application: western blot, ELISA, immunohistochemistry, flow cytometry
citations: 1
Western Blot of Mouse anti-AKT antibody. Lane 1: unstimulated NIH/3T3 cell lysates. Lane 2: PDGF stimulated NIH/3T3 cell lysates. Load: 10 µg per lane. Primary antibody: AKT antibody at 1:400 for overnight at 4°C. Secondary antibody: HRP conjugated Gt-a-Mouse IgG (p/n 610-103-121) was used at a 1:40,000 dilution for 1 h at 4° C with FemtoMax™ enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Block: 5% BLOTTO (p/n B501-0500 in TBS for 2h at RT. Observed size: ~56 kDa for AKT. Other band(s): none.
Western Blot of Mouse anti-AKT antibody. Lane 1: unstimulated NIH/3T3 cell lysates. Lane 2: PDGF stimulated NIH/3T3 cell lysates. Load: 10 µg per lane. Primary antibody: AKT antibody at 1:400 for overnight at 4°C. Secondary antibody: HRP conjugated Gt-a-Mouse IgG (p/n 610-103-121) was used at a 1:40,000 dilution for 1 h at 4° C with FemtoMax™ enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Block: 5% BLOTTO (p/n B501-0500 in TBS for 2h at RT. Observed size: ~56 kDa for AKT. Other band(s): none.
quantity: 100 µg
price: 369.00 USD
to the supplier - mouse monoclonal (17F6.B11)
reactivity: human, mouse, rat, chimpanzee
application: western blot, ELISA, immunohistochemistry - paraffin section - mouse monoclonal (14E5.16C8.25F6)
reactivity: human, mouse, rat, chimpanzee
application: western blot, ELISA, immunohistochemistry, immunoprecipitation - mouse monoclonal (18F10.E5)
reactivity: human, mouse, rat, chimpanzee
application: western blot
gene information - chimpanzee AKT1
- synonym:AKT2
- description:AKT serine/threonine kinase 1
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- AKT1 antibody
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