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- domestic rabbit polyclonal
reactivity: human, chicken
application: western blot, ELISA, immunohistochemistry
Western blot using Rockland's Affinity Purified anti-SLIT-2 antibody shows detection of a band at ~165 kDa (lane 1) corresponding to SLIT-2 present in a chicken spinal cord whole cell lysate (arrowhead). Approximately 30 ?g of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,350. Reaction occurred overnight at 4° C followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
quantity: 100 µg
price:
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat, chicken
application: western blot, immunocytochemistry, immunohistochemistry - frozen section
gene information - chicken slit 2
- synonym:Slit
- description:slit guidance ligand 2
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product type
- slit 2 antibody
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