castor bean LOC8287993 antibody | 1 product cited in the literature; 7 total from 3 suppliers

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Ricin alpha Monoclonal Antibody (RA999)
Invitrogen
catalog: MA1-74225

mouse monoclonal (RA999)
reactivity: castor bean
application: western blot, ELISA
citations: 1

quantity: 200µg
price: US 390
to the supplier
Ricin beta Monoclonal Antibody (RB999)
Invitrogen
catalog: MA1-74237

mouse monoclonal (RB999)
reactivity: castor bean
application: western blot, ELISA

quantity: 200µg
price: US 390
to the supplier
Mouse anti RICIN A CHAIN
Bio-Rad
catalog: MCA2865

mouse monoclonal (RA999)
reactivity: castor bean
application: western blot, ELISA

Published customer image: . Mouse anti ricin A chain antibody, clone RA999 used for the detection of wild type ricin following incubation with HEK293 cells in vitro by western blotting under non-reducing conditions. Image caption: . DHF ricin and IHF ricin show decreased binding to the cell surface and increased degradation in low pH vesicles. (A) HEK293 cells after 3 hours incubation with wild-type ricin, IHF ricin or DHF ricin run on SDS/PAGE under non-reducing conditions. A representative membranes after Western blot with anti-RTA antibodies are shown. Western blots with anti-tubulin antibodies were performed to show equal loading control. Data from three independent experiments are presented. RTA wt is marked as 1, other results are relative to this control. (B) Cells were incubated with wild-type ricin, IHF ricin or DHF ricin for 30 min at 0°C. Binding was measured as described in Methods. A representative membranes after Western blot with anti-RTA antibodies are shown. Western blots with anti-tubulin antibodies were performed to show equal loading control. Data from three independent experiments are presented. RTA wt is marked as 1, other results are relative to this control. (C) Coomasie Blue-stained 12% SDS/PAGE gels showing the stability of 500 ng of wild-type ricin, and modified ricin IHF and DHF after 30 min incubation at 0°C. (D) Cells were treated with or without bafilomycin A1 (0.1 μM) and with wild-type ricin, IHF ricin or DHF ricin. The amounts of ricin that remain in the cell after degradation was analyzed after SDS-PAGE run under non-reducing conditions (see Methods). Representative membranes after Western blot with anti-RTA antibodies are shown. Western blots with anti-tubulin antibodies were performed to show equal loading control. Data from three independent experiments are presented. Ricin wt, ricin IHF and ricin DHF in each graph are marked as 1, other results are relative to this control. All degraded forms of ricin were analyzed. (E) Tyrosine sulfated wild-type ricin sulf-1, and modified ricin sulf-1: IHF or DHF in cells after 3 hours incubation with Na235SO4 and further 3 h incubation with the toxins, run on SDS/PAGE under non-reducing conditions. Control experiments revealed equal total sulfation of proteins in cell lysates. A representative autoradiogram is shown. From: Soko owska I, Pi ka ES, Sandvig K, W grzyn G, S omi ska-Wojew dzka M. Hydrophobicity of protein determinants influences the recognition of substrates by EDEM1 and EDEM2 in human cells. BMC Cell Biol. 2015 Feb 6;16:1 .
quantity: 0.2 mg
price: 325 USD
to the supplier
Mouse anti RICIN B CHAIN
Bio-Rad
catalog: MCA2866

mouse monoclonal (RB999)
reactivity: castor bean
application: western blot, ELISA

quantity: 0.2 mg
price: 325 USD
to the supplier
MOUSE ANTI RICIN A CHAIN
MyBioSource
catalog: MBS216335

mouse monoclonal (RA999)
reactivity: castor bean
application: western blot, ELISA, enzyme immunoassay

quantity: 0.2 mg
price: 390 USD
to the supplier
MOUSE ANTI RICIN B CHAIN
MyBioSource
catalog: MBS216336

mouse monoclonal ([RB999])
reactivity: castor bean
application: western blot, ELISA, enzyme immunoassay

quantity: 0.2 mg
price: 430 USD
to the supplier
Ricin antibody
MyBioSource
catalog: MBS532353

mouse monoclonal ([CP37])
reactivity: castor bean
application: western blot, ELISA, enzyme immunoassay

quantity: 1 mg
price: 645 USD
to the supplier

gene information - castor bean LOC8287993

- synonym:ricin-like; pre-propolypeptide (AA -35 to 541); preproricin; ricin A chain; ricin B-chain
- description:ricin-like

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LOC8287993 antibody

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