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- domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs, bovine
application: western blot, ELISA
Western blot using Rockland's Rabbit anti-SAE1 pS185 antibody shows detection of phosphorylated SAE1. Left lane (-) contains 20 µg human HeLa whole cell protein. Right lane (+) contains 20 µg human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephos-phorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with the primary antibody diluted to 1:1,000 at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houstin, TX
quantity: 100 µg
price: 369 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs, bovine
application: western blot, ELISA 
Western blot using Rockland's Rabbit anti-SAE1 antibody shows detection of SAE1. Left lane (-) contains 20 µg human HeLa whole cell protein. Right lane (+) contains 20 µg human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephosphorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with the primary antibody, Anti-SAE1, diluted to 2 µg/mL at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houstin, TX.
quantity: 100 µg
price: 369 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs, bovine
application: western blot, ELISA, immunocytochemistry - domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs, bovine
application: western blot, ELISA
gene information - bovine SAE1
- description:SUMO1 activating enzyme subunit 1
do you know?
- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- SAE1 antibody
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