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- domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs, chicken, bovine
application: western blot, immunocytochemistry
Western blot analysis of PDGFR alpha expression in HepG2 (A), NIH3T3 (B), Saos2 (C), HeLa (D), A431 (E) whole cell lysates.
Immunofluorescent analysis of PDGFR alpha staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity: 0.03 mL
price: 155 USD
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gene information - bovine PDGFRA
- description:platelet derived growth factor receptor alpha
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product type
- PDGFRA antibody
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