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- domestic rabbit monoclonal (SZ22-01)
reactivity: human, mouse, rat, dogs, bovine
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of MEK1 on different lysates with Rabbit anti-MEK1 antibody (ET1603-20) at 1/2,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si MEK1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 43 kDa Observed band size: 45 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-20) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of MEK1 on different lysates with Rabbit anti-MEK1 antibody (ET1603-20) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: Mouse brain tissue lysate (30 µg/Lane) Lane 5: Rat skeletal muscle tissue lysate (30 µg/Lane) Predicted band size: 43 kDa Observed band size: 45 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-20) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells labeling MEK1 with Rabbit anti-MEK1 antibody (ET1603-20) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MEK1 antibody (ET1603-20) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs, chicken, bovine
application: western blot 
Western Blot: MEK1 Antibody [NBP2-24542] - Analysis of MEK1/MEK2 phosphorylation in A375 whole cell lysate. Lane A shows phospho MEK1/MEK2 (upper band), and the weakly detected nonphosphorylated form (lower band), using MEK1 phospho antibody at 3 ug/ml. Lane B shows the nonphosphorylated form of MEK1/2 using this antibody.
quantity: 0.05 mg (also 0.025 mg)
price: 279 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs, chicken, bovine
application: western blot - domestic rabbit monoclonal
reactivity: human, mouse, rat, bovine
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
gene information - bovine MAP2K1
- description:mitogen-activated protein kinase kinase 1
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- MAP2K1 antibody
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