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- domestic rabbit polyclonal
reactivity: human, mouse, rat, bovine, pigs
application: western blot
citations: 3 - domestic rabbit polyclonal
reactivity: human, mouse, rat, bovine, zebrafish
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
Western blot analysis of STAT1 (pY701) expression in HEK293T (A), NIH3T3 (B), mouse brain (C), COS7 (D) whole cell lysates.
Immunohistochemical analysis of STAT1 (pY701) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of STAT1 (pY701) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
quantity: 0.03 mL
price:
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat, bovine
application: western blot, immunohistochemistry, immunocytochemistry
Western blot analysis of FOXO1 (pS329) expression in HEK293T (A), SHSY5Y (B), NIH3T3 (C), mouse brain (D) whole cell lysates.
Immunohistochemical analysis of FOXO1 (pS329) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of FOXO1 (pS329) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
quantity: 0.03 mL
price:
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat, chicken, bovine, zebrafish
modification: phosphorylated
application: western blot
gene information - bovine FOXO1
- synonym:FOXO1A
- description:forkhead box O1
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
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- FOXO1 antibody
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