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- domestic rabbit polyclonal
reactivity: human, mouse, chicken, bovine
application: western blot, immunohistochemistry, immunocytochemistry
Western blot analysis of HuB expression in K562 (A), HeLa (B) whole cell lysates.
Immunohistochemical analysis of HuB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of HuB staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
quantity: 0.03 mL
price: 155 USD
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gene information - bovine ELAVL2
- description:ELAV like RNA binding protein 2
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- ELAVL2 antibody
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