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- mouse monoclonal (1H3.B8)
reactivity: human, mouse, rat, bovine
application: western blot, ELISA, immunocytochemistry
citations: 2
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha A Crystallin Monoclonal Antibody, Clone 1H3.B8 (SMC-142). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha A Crystallin Monoclonal Antibody (SMC-142) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha A Crystallin Antibody. (D) Composite.
quantity: 200 µg
price:
to the supplier - mouse monoclonal (1H3.B8)
reactivity: human, mouse, rat, bovine
application: western blot, ELISA, immunocytochemistry
citations: 1 - mouse monoclonal (1H3.B8)
reactivity: human, mouse, rat, bovine
application: western blot, ELISA 
Western Blot of mouse Anti-Alpha A Crystallin Antibody. Lane 1: AlphaA Crystallin Lane2: AlphaB Crystallin. Load: 20ug per lane. Primary antibody: AlphaA-Crystallin 1:1000 for overnight at 4°C . Secondary antibody: Goat anti-Mouse IgG HRP antibody at 1:40,000 for 45 min at RT. Block: 5% Blotto overnight at 4°C. Predicted/Observed size: 19.9 kDa, 20 kDa for Alpha A.
quantity: 200 µg
price:
to the supplier
gene information - bovine CRYAA
- description:crystallin alpha A
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- CRYAA antibody
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