Your Filters
product
- mouse monoclonal (F1-B4)
reactivity: Zaire ebolavirus
application: ELISA - mouse monoclonal (A2-G7)
reactivity: Zaire ebolavirus
application: ELISA - mouse monoclonal (B6-A8)
reactivity: Zaire ebolavirus
application: ELISA
VP40 Antibody (HA601066) in indirect ELISA. Indirect ELISA analysis of VP40 was performed by coating wells of a 96-well plate with 50 µl per well of VP40 antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 50 µl per well of a mouse VP40 monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:15,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 360.00 USD
to the supplier
gene information - Zaire ebolavirus VP40
- description:matrix protein
do you know?
- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- VP40 antibody
linkout
questions and comments