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- domestic rabbit polyclonal
reactivity: human, mouse, rat, Xenopus laevis
application: western blot, ELISA
Figure 1. Western blot analysis of Semaphorin 3A using anti-Semaphorin 3A antibody (A01761-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,. Lane 2: mouse brain tissue lysates,. Lane 3: human U-87MG whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Semaphorin 3A antigen affinity purified polyclonal antibody (Catalog # A01761-1) at 0.5 ug/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Semaphorin 3A at approximately 95KD. The expected band size for Semaphorin 3A is at 89KD.
quantity: 100 ug/vial
price: 315 USD
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gene information - Xenopus laevis sema3a.L
- synonym:sema3a; sema3a-A
- description:semaphorin 3A L homeolog
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product type
- sema3a.L antibody
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