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- rat monoclonal (YOL1/34)
reactivity: Saccharomyces cerevisiae S288c, Candida albicans, human, mouse, rat, dog
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section
citations: 3 - rabbit monoclonal (CI-20)
reactivity: Saccharomyces cerevisiae S288c
application: western blot
Western blot analysis of alpha Tubulin expression in Saccharomyces cerevisiae (yeast) cell lysate (M30932). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUB1 monoclonal antibody (Catalog # M30932) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUB1
quantity: 100 ug/vial
price: 299 USD
to the supplier - rat monoclonal (YOL1/34)
reactivity: Saccharomyces cerevisiae S288c, human
application: western blot, ELISA, radioimmunoassay, immunohistochemistry - paraffin section, immunohistochemistry - frozen section - rat monoclonal (YL1/2)
reactivity: Saccharomyces cerevisiae S288c, human
application: western blot, ELISA, immunoprecipitation, radioimmunoassay, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
gene information - Saccharomyces cerevisiae S288c TUB1
- synonym:alpha-tubulin TUB1
- description:alpha-tubulin TUB1
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product type
- TUB1 antibody
- TUB1 cDNA
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