Your Filters
product
- rabbit polyclonal
reactivity: Saccharomyces cerevisiae S288c
application: western blot, ELISA, chromatin immunoprecipitation - rabbit polyclonal
reactivity: Saccharomyces cerevisiae S288c, human
application: western blot, ELISA, chromatin immunoprecipitation
Chromatin Immunoprecipitation (ChIP) using Rockland's Affinity Purified Mre11 (S. cerevisiae) antibody. A yeast strain containing the HO endonuclease gene controlled by a galactose-inducible promoter (uninduced 0 m lanes) was shifted into galactose containing medium (induced 60 m lanes). After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of sheared chromatin. Chromatin was immunoprecipitated with the antibody at the stated dilutions. Immunocomplexes were captured using polyacrylamide bead linked secondary antibodies. The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower arrow) and 67 kb from the break (upper band, control locus). PCR products were displayed on a polyacrylamide gel, stained with SyBR Green® (Invitrogen), and detected using a Fuji scanning fluorometer. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.
quantity: 100 µg
price: 359.00 USD
to the supplier
gene information - Saccharomyces cerevisiae S288c MRE11
- synonym:NGS1; RAD58; XRS4; MRX complex nuclease subunit
- description:MRX complex nuclease subunit
do you know?
- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
product type
- MRE11 antibody
species
linkout
questions and comments