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- domestic rabbit polyclonal
reactivity: Saccharomyces cerevisiae S288C
application: western blot, ELISA, immunoprecipitation, chromatin immunoprecipitation
citations: 4
Anti-SUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by western blot against several constructs of SUMO-GFP fusion proteins after cleavage by proteases in insect cell protein extracts. These constructs contained various linkers between the SUMO and GFP portion of the fusion proteins. Each sample was run twice. The left lanes each contain 2 ug E.coli expressed and purified SUMO-GFP fusion proteins after incubation with lysed cells (50 ug total protein) for 1 h. The right lanes contain the same fusion proteins incubated with the lysate in the presence of 2% SDS. After probing with anti-GFP antibodies the membranes were stripped of antibody using SDS-DTT solution for 30 m at 60° C and were then re-probed using the anti-SUMO antibody at a 1:1000 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG (code # 611-703-127) for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results.
quantity: 500
price: https://www.rockland.com/search/?searchString=200-401-428
to the supplier - mouse monoclonal (4F2.F5.G2)
reactivity: Saccharomyces cerevisiae S288C
application: western blot, ELISA, immunohistochemistry
citations: 1 
Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC)-like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch.
quantity: 100 µg
price:
to the supplier - domestic rabbit monoclonal
reactivity: Saccharomyces cerevisiae S288C
application: western blot, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: Saccharomyces cerevisiae S288C
application: western blot, ELISA - mouse monoclonal (4F2.F5.G2)
reactivity: Saccharomyces cerevisiae S288C
application: western blot, ELISA 
Immunoblot of ySUMO fusion protein. Anti-ySUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by immunoblot against a SUMO-GFP fusion protein (lane 2). While the actual molecular weight of the fusion protein is 39 kDa, the protein migrates as a 49 kDa band (arrowhead). No reactivity is seen for lane 1 which contains His-tagged GFP protein. The membrane was blocked using BLOTTO. Primary antibody was used at a 1:1,000 dilution in BLOTTO. The membrane was washed and reacted with a 1:10,000 dilution of IRDye (R) 800 Conjugated Affinity Purified Goat-anti-Mouset IgG (H&L) MX10 (800 nm channel). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (lane M, 700 nm channel). Other detection systems will yield similar results.
quantity: 100 µg
price:
to the supplier
gene information - Saccharomyces cerevisiae S288C SMT3
- description:SUMO family protein SMT3
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product type
- SMT3 antibody
- SMT3 protein
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