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- domestic rabbit polyclonal
reactivity: Saccharomyces cerevisiae S288C
application: western blot, ELISA, immunoprecipitation
Western blot using Rockland's affinity purified anti-S.cerevisiae Mer2 antibody shows detection of phosphorylated and unphosphorylated Mer2 in wild type, phosphatase treated and mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody is reactive with both phosphor-ylated and unphosphorylated Mer2 at the S30 position. The primary antibody was used at a 1:5,000 dilution. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.
quantity: 100 µg
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to the supplier - domestic rabbit polyclonal
reactivity: Saccharomyces cerevisiae S288C
application: western blot, ELISA, immunoprecipitation 
Western blot using Rockland's affinity purified anti-S.cerevisiae Mer2 pS30 antibody shows detection of phosphorylated Mer2 in whole cell extracts. Cells were either wild type (+) or contained mer2 deletions (D). Extracts were prepared from cells 4hr after initiation of meiosis. Proteins were obtained using TCA precipitation. The primary antibody was used at a 1:7,500 dilution. Secondary antibody was used at 1:5,000 dilution. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.
quantity: 100 µg
price:
to the supplier - domestic rabbit polyclonal
reactivity: Saccharomyces cerevisiae S288C
application: western blot, ELISA, immunoprecipitation - domestic rabbit polyclonal
reactivity: Saccharomyces cerevisiae S288C
application: western blot, ELISA, immunoprecipitation - domestic rabbit polyclonal
reactivity: Saccharomyces cerevisiae S288C
application: western blot, ELISA, immunoprecipitation
gene information - Saccharomyces cerevisiae S288C REC107
- synonym:MER2
- description:Rec107p
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product type
- REC107 antibody
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