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- mouse monoclonal (F31 P2 A5)
reactivity: human, mouse, Rhesus monkey
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 4
Immunohistochemistry was performed on normal biopsies of deparaffinized Human prostate tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a TIMP1 monoclonal antibody (Product # MA1-773) at a dilution of 1:20 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
quantity: 100µg
price: 329 USD
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gene information - Rhesus monkey TIMP1
- synonym:TIMP-1; metalloproteinase inhibitor 1; tissue inhibitor of matrix metalloproteinase-1; tissue inhibitor of metalloproteinases 1
- description:TIMP metallopeptidase inhibitor 1
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- TIMP1 antibody
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