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- domestic rabbit monoclonal (SR41-04)
reactivity: , Escherichia coli O157:H7 str. Sakai
application: western blot, immunoprecipitation
Western blot analysis of Maltose Binding Protein on recombinant MBP-tag protein lysates using anti-Maltose Binding Protein antibody at 1/1,000 dilution.
MBP tag was immunoprecipitated in 2µg MBP Tag fusion protein lysate with ET1602-46 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using ET1602-46 at 1/1,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 60 mins at room temperature. Lane 1: MBP Tag fusion protein lysate (input). Lane 2: ET1602-46 IP in MBP Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST
quantity: 100μl
price: 385.00 USD
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gene information - Escherichia coli O157:H7 str. Sakai malE
- description:maltose ABC transporter periplasmic protein
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product type
- malE antibody
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