Drosophila melanogaster Hsp70Aa antibody | 5 products in Validated Antibody Database; 5 cited in the literature; 47 total from 4 suppliers

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HSP70 Monoclonal Antibody (3A3)
Invitrogen
catalog: MA3-006

total number of products: 4

mouse monoclonal (3A3)
reactivity: snow fleas, Marenzelleria viridis, common limpet, Eyprepocnemis plorans, crucian carp, Saccharomycetales, Pacific oyster, lobsters, Meganyctiphanes norvegica, roach minnow, red rock crab, Artemia franciscana, holothurians, Japanese rice fish, Asian green mussel, sea monkeys, eastern oyster, Chlamydomonas reinhardtii, giant tiger prawn, human, mouse, rat, dogs, swine, Drosophila melanogaster, Caenorhabditis elegans, rhesus macaque
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, blocking or activating experiments
citations: 83

Immunofluorescent analysis of Heat Shock Protein 70 using Heat Shock Protein 70 Monoclonal antibody (3A3) (Product# MA3-006) shows staining in HeLa cells. Heat Shock Protein 70 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 70 (Product# MA3-006) at a dilution of 1:100-1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

Western blot analysis of Heat Shock Protein 70 (Hsp70) was performed by loading 50ug of the indicated whole cell lysates and 15ul of PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a Hsp70 monoclonal antibody (Product # MA3-006) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
quantity: 50µg
price: US 390
to the supplier
HSP70 Monoclonal Antibody (5A5)
Invitrogen
catalog: MA3-007

total number of products: 4

mouse monoclonal (5A5)
reactivity: California mussel, forest day mosquito, hamsters, scallops, Lycaena tityrus, African green monkey, Pacific oyster, human, mouse, rat, dogs, Drosophila melanogaster
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments
citations: 48

Western blot analysis of Heat Shock Protein 70 (HSP70) was performed by loading 50µg of the indicated whole cell lysates and 15?l of PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP70 monoclonal antibody (Product # MA3-007) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).

Immunofluorescent analysis of Heat Shock Protein 70 using Heat Shock Protein 70 Monoclonal antibody (5A5) (Product# MA3-007) shows staining in HeLa cells. Heat Shock Protein 70 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 70 (Product# MA3-007) at a dilution of 1:100-1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

Immunohistochemistry was performed on cancer biopsies of deparaffinized Human prostate carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Heat Shock Protein 70 (MA3-007) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
quantity: 250µL
price: US 409
to the supplier
HSP70 Monoclonal Antibody (2A4)
Invitrogen
catalog: MA3-008

total number of products: 4

mouse monoclonal (2A4)
reactivity: human, mouse, rat, Drosophila melanogaster
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 11

Immunofluorescent analysis of Heat Shock Protein 70 (Hsp70) in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 70 (Hsp70) monoclonal antibody (Product # MA3-008) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Heat Shock Protein 70 (Hsp70) staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Western blot analysis of Heat Shock Protein 70 (HSP70) was performed by loading 50ug of the indicated whole cell lysates and 15ul PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP70 monoclonal antibody (Product # MA3-008) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgM-HRP secondary antibody (Product # 31440) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).

Immunohistochemistry was performed on normal biopsies of deparaffinized Human breast tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Heat Shock Protein 70 (MA3-008) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
quantity: 100µL
price: US 390
to the supplier
Anti-Hsp70 antibody [C92F3A-5]
Abcam
catalog: ab47455

mouse monoclonal (C92F3A-5)
reactivity: African green monkey, human, mouse, rat, dogs, bovine, Drosophila melanogaster
application: western blot, ELISA, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 24

quantity: 100 µl
price:
to the supplier
Hsp70 antibody [2A4]
Abcam
catalog: ab5442

mouse monoclonal (2A4)
reactivity: Austrofundulus limnaeus, human, mouse, rat, Drosophila melanogaster
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 10

quantity: 50 ul
price:
to the supplier
HSPA1A monoclonal antibody, clone 5A5
Abnova
catalog: MAB11390

total number of products: 36

mouse monoclonal (5A5)
reactivity: human, mouse, rat, Drosophila melanogaster
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

quantity: 100 ug
price:
to the supplier
HSPA1A monoclonal antibody, clone 5A5 (ATTO 390)
Abnova
catalog: MAB11391

total number of products: 36

mouse monoclonal (5A5)
reactivity: human, mouse, rat, Drosophila melanogaster
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

quantity: 100 ug
price:
to the supplier
HSPA1A monoclonal antibody, clone 5A5 (ATTO 488)
Abnova
catalog: MAB11392

total number of products: 36

mouse monoclonal (5A5)
reactivity: human, mouse, rat, Drosophila melanogaster
conjugate: ATTO 488
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

quantity: 100 ug
price:
to the supplier
Anti-HSP70 monoclonal antibody, clone D03G4B-6 [DyLight® 488]
Creative Biomart
catalog: DCABH-463

total number of products: 5

mouse monoclonal (D03G4B-6)
reactivity: human, mouse, rat, bovine, Drosophila melanogaster
conjugate: DyLight488
application: flow cytometry

quantity:
price:
to the supplier
Anti-HSP70 monoclonal antibody, clone D03G4B-6 [PE]
Creative Biomart
catalog: DCABH-470

total number of products: 5

mouse monoclonal (D03G4B-6)
reactivity: human, mouse, rat, bovine, Drosophila melanogaster
conjugate: PE
application: flow cytometry

quantity:
price:
to the supplier
Anti-HSP70 monoclonal antibody, clone D03G4B-6 [HRP]
Creative Biomart
catalog: DCABH-7598

total number of products: 5

mouse monoclonal (D03G4B-6)
reactivity: human, mouse, rat, bovine, Drosophila melanogaster
conjugate: HRP
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

quantity:
price:
to the supplier