Chinese hamster Hsp90b1 antibody | 1 product in Validated Antibody Database; 6 cited in the literature; 7 total from 1 supplier

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GRP94 Monoclonal Antibody (9G10)
Invitrogen
catalog: MA3-016

total number of products: 7

rat monoclonal (9G10)
reactivity: Chinese hamster, human, mouse, rat, chicken, cow
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 19

Immunofluorescent analysis of Glucose-Regulated Protein 94 using Glucose-Regulated Protein 94 Monoclonal Antibody (9G10) (Product# MA3-016 ) shows staining in C6 Cells. Glucose-Regulated Protein 94 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucose-Regulated Protein 94 (Product# MA3-016 ) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse breast tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Glucose Regulated Protein 94 (MA3-016) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
quantity: 100µL
price: US 390
to the supplier
KDEL Polyclonal Antibody
Invitrogen
catalog: PA1-013

total number of products: 7

rabbit polyclonal
reactivity: Chinese hamster, human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 8

quantity: 100µg
price: US 390
to the supplier
GRP94 Monoclonal Antibody (9G10.F8.2)
Invitrogen
catalog: MA5-14592

total number of products: 7

rat monoclonal (9G10.F8.2)
reactivity: Chinese hamster, Domestic guinea pig, human, mouse, rat, dog, chicken, cow, pig, sheep, common platanna, rabbit, equine
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 4

quantity: 500µL
price: US 325
to the supplier