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- mouse monoclonal (3C12)
reactivity: Chinese hamster, human, mouse, rat, dog, cow
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 63
Immunofluorescent analysis of Phalloidin (orange) and Ezrin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an Ezrin monoclonal antibody (Product # MA5-13862) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
HeLa cells were lysed 72 hours after transfection. Cells were transfected with Transfection Reagent alone (Lane 1), 100nM ON-TARGETplus siCONTROL Non-Targeting Pool (Lane 2), or 100nM ONTARGETplus VIL2 siRNA (Lane 3). Western blot data for GAPDH is included as a control for equal protein loading.
Immunohistochemistry was performed on formalin-fixed, paraffin-embedded human lung tissue. To expose target proteins, heat induced epitope retrieval (HIER) was performed by boiling tissues in 10mM sodium citrate buffer (pH 6.0) for 20 minutes. Following antigen retrieval, tissues were blocked and probed with an Ezrin monoclonal antibody (Product # MA5-13862) at a concentration of 2ug/ml. Detection was performed using an HRP-conjugated anti-mouse IgG secondary antibody and DAB chromogenic substrate.
quantity: 500µL
price: 380 USD
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gene information - Chinese hamster Ezr
- synonym:ezrin
- description:ezrin
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product type
- Ezr antibody
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