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- rabbit polyclonal
reactivity: Borrelia burgdorferi B31
application: western blot, ELISA
Western blot showing detection of 0.1 µg of recombinant Flagellin protein. Lane 1: Molecular weight markers. Lane 2: MBP-Flagellin fusion protein (arrowhead at expected MW: 76.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc™ MP 4000 imaging system (Bio-Rad).
quantity: 100 µg
price: 369 USD
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gene information - Borrelia burgdorferi B31 BB_0147
- description:flagellin
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product type
- BB_0147 antibody
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