What is the control for Endostatin antibody and Antigen retrieval method ?


Labome reply

Hello,

The recommended Antigen Retrieval method is: Boil slides in 0.01M sodium citrate buffer (pH6) at 100°C for 15-20 minutes. Remove the slides from heat and allow them to stand at RT in buffer for 20 minutes.Rinse twice with Tris-Buffered Saline + Tween (TBST) for 5 minutes at RT.

Colon and stomach tissue should be suitable positive controls. We also have validation data posted on the Product Page using human kidney carcinoma.

Please let me know if you have any questions or concerns.

Best regards,
David




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