Labome reply
Thanks for your feedback. The article used both 7B and 7A antibodies from Santa Cruz. Please see the excerpt here.
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Western blotting was performed on samples grown as above and treated as indicated. Protein isolation, electrophoresis and transfer to nitrocellulose (Invitrogen) was performed as described previously (McNeill-Blue et al., 2006). HRP-based detection was subsequently carried out with either ECL (GEH Amersham, Piscataway, NJ) or SuperSignal (Pierce, Rockford, IL) reagent and used the following antibodies: wnt7b (Q-13, Santa Cruz Biotechnology, Santa Cruz, CA), wnt7a/b (H-40, Santa Cruz), wnt7a (K-15, Santa Cruz), actin (MAb1501R, Chemicon, Temecula, CA), phosphorylated cJun (9164, Cell Signaling, Danvers, MA), phosphorylated JNK (9251, Cell Signaling), total JNK (9252, Cell Signaling), β-catenin (9581, Cell Signaling), donkey anti-rabbit IgG-HRP (GEH Amersham) and sheep anti-mouse IgG-HRP (GEH Amersham).
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