PI3K Cascade
gene
reagent
sample publication
sample review
total:38
The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. 2011 to the paper
Cell Signaling Technology anti-4E-BP1 and phospho-T37/T46 4E-BP1, phospho-S65 4E-BP1 were used to perform western blot in order to show that how growth factor signaling is inhibited by mTOR through mTOR-regulated phosphoproteome. more
total:14
The ancient drug salicylate directly activates AMP-activated protein kinase. 2012 to the paper
Cell Signalling anti-pAMPK was used to perform western blot in order to show that AMPK could be activated by salicylate directly. more
total:1
Identification of the serine 307 of LKB1 as a novel phosphorylation site essential for its nucleocytoplasmic transport and endothelial cell angiogenesis. 2009 to the paper
Epitomics MO25 rabbit monoclonal antibody was used for Western blotting in order to investigate the effect of LKB1 on nucleocytoplasmic transport.
total:1
Histone acetylation-independent effect of histone deacetylase inhibitors on Akt through the reshuffling of protein phosphatase 1 complexes. 2005 to the paper
Alpha Diagnostic International rabbit anti-human C-terminal modulator protein (CTMP) antibody was used in western blot to study the effect of the interaction between histone deacetylase inhibitors and protein phosphatase 1 complexes on Akt in human U87MG cells.
total:3
Chemical rescue of a mutant enzyme in living cells. 2006 to the paper
Santa Cruz Biotechnology anti-Dok-1 antibody was used in western blot to study the protein tyrosine kinase Src R388A mutant rescued in live cells with the use of the small molecule imidazole. more
total:4
mTORC1-mediated cell proliferation, but not cell growth, controlled by the 4E-BPs. 2010 to the paper
Cell Signaling Technology anti-phospho-eIF4B antibody was used to carry out western blot analysis in order to investigate the precise contribution of 4E-BPs to mTORC1 signaling. more
total:37
Control of translation and miRNA-dependent repression by a novel poly(A) binding protein, hnRNP-Q. 2013 to the paper
BD BioSciences anti-eIF4E antibody was used to perform western blot in order to check the protein levels when hnRNP-Q was knocked down. more
total:5
Global analysis of TDP-43 interacting proteins reveals strong association with RNA splicing and translation machinery. 2010 to the paper
Santa Cruz Biotechnology anti-EIF4G antibody was used to perform immunocytochemistry in order to study the interaction between TDP-43 and proteins that regulate RNA metabolism. more
total:20
Endothelial-derived FGF2 contributes to the progression of pulmonary hypertension in humans and rodents. 2009 to the paper
Chemicon rabbit polyclonal anti-FGF2 antibody was used in western blot to study the role for endothelial-derived FGF2 in the progression of pulmonary hypertension in humans and rodents. more
total:2
HSulf-2, an extracellular endoglucosamine-6-sulfatase, selectively mobilizes heparin-bound growth factors and chemokines: effects on VEGF, FGF-1, and SDF-1. 2006 to the paper
R&D Systems goat polyclonal anti-human FGF-1 antibody was used in ELISA to study the HSulf-2 which selectively mobilizes heparin-bound growth factors and chemokines. more
total:2
Androgen receptor-negative human prostate cancer cells induce osteogenesis in mice through FGF9-mediated mechanisms. 2008 to the paper
R&D Systems FGF9 antibody was used in immunohistochemistry at 5 ug/ml in order to study the role of androgen receptor null cells in prostate cancer bone metastases treatment more
total:17
Facilitation of AMPA receptor synaptic delivery as a molecular mechanism for cognitive enhancement. 2012 to the paper
Biosource International anti-FGFR-1 (pYpY653/654) antibodies were used to perform western blot and ELISA analyses in order to study the effect of the facilitation of AMPA receptor synaptic delivery on cognitive enhancement. more
total:9
Endothelial function of von Hippel-Lindau tumor suppressor gene: control of fibroblast growth factor receptor signaling. 2008 to the paper
Santa Cruz rabbit polyclonal anti-FGFR2 was used to perform western blot in order to show that VHL tumor supppressor gene has endothelial function through fibroblast growth factor receptor signaling. more
total:7
Transforming fusions of FGFR and TACC genes in human glioblastoma. 2012 to the paper
Santa Cruz mouse anti-FGFR3 was used at 1:1000 to perform immunohistochemistry and immunocytochemistry in order to show that FGFR-TACC fusion happened in specific GBM patients. more
total:5
Resident hepatocyte fibroblast growth factor receptor 4 limits hepatocarcinogenesis. 2009 to the paper
Santa Cruz Biotechnology anti-FGFR4 antibody was used to carry out western blot analysis in order to investigate whether FGFR4 plays a role in promoting the progression of hepatocellular carcinoma. more
total:6
Transforming fusions of FGFR and TACC genes in human glioblastoma. 2012 to the paper
Santa Cruz rabbit anti-FRS was used at 1:250 to perform immunohistochemistry and immunocytochemistry in order to show that FGFR-TACC fusion happened in specific GBM patients. more
total:9
Pak4, a novel Gab1 binding partner, modulates cell migration and invasion by the Met receptor. 2009 to the paper
Upstate Biotechnology polyclonal anti-Gab1 antibody was used in western blot to identify Pak4 as a novel Gab1-interacting protein downstream from the Met receptor and to study its function. more
total:36
PAX5 is expressed in small-cell lung cancer and positively regulates c-Met transcription. 2009 to the paper
Santa Cruz Biotechnology rabbit polyclonal against Ron antibody was used in western blot in order to demostrate the expression of PAX 5 and the regulation for c-Met transcription in small cell lung cancer. more
total:37
Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. 2011 to the paper
Invitrogen anti-phospho-IRS (Y612) was used to perform western blot in order to show that Grb10 was indentified as a negative regulator of insulin signaling by phosphoproteomic analysis. more
total:13
Genetic deficiency of glycogen synthase kinase-3beta corrects diabetes in mouse models of insulin resistance. 2008 to the paper
Upstate Irs2 antibody was used in western blot in order to study the role of Gsk-3beta in control of beta-cell mass in insulin-resistant diabetic models more
total:7
Pancreatic LKB1 deletion leads to acinar polarity defects and cystic neoplasms. 2008 to the paper
Santa Cruz Biotechnology anti-Lkb1 antibody was used to perform western blot and immunohistochemistry in order to study the acinar polarity defects and cystic neoplasms induced by Lkb1-deficient in pancreas. more
total:33
A gene signature-based approach identifies mTOR as a regulator of p73. 2008 to the paper
Santa Cruz polyclonal anti-mTOR (N-19 (alpha-FRAP) was used to perform western blot in order to show that mTOR is identified as a regulator of p73 by a gene signature-based approach. more
total:26
Protein kinase activity of phosphoinositide 3-kinase regulates cytokine-dependent cell survival. 2013 to the paper
Cell Signalling anti-p110alpha was used to perform immunoprecipitation to the role of phosphoinositide 3-kinase in the regulation of cytokine-dependent cell survival more
total:60
mTORC1 senses lysosomal amino acids through an inside-out mechanism that requires the vacuolar H(+)-ATPase. 2011 to the paper
Cell Signaling anti-S6K1 and phospho-T389 S6K1 was used to perform western blot in order to show that vacuolar H+-ATPase is involved in mTOR pathway for mTORC1 translocation and activation. more
total:43
Protein kinase activity of phosphoinositide 3-kinase regulates cytokine-dependent cell survival. 2013 to the paper
Upstate anti-p85 pAb was used to perform immunoprecipitation to the role of phosphoinositide 3-kinase in the regulation of cytokine-dependent cell survival more
total:1
Suppression of cyclic GMP-specific phosphodiesterase 5 promotes apoptosis and inhibits growth in HT29 cells. 2005 to the paper
Santa Cruz Biotech anti-PDE3B antibody was used in western blot to study the suppression of cyclic GMP-specific phosphodiesterase 5 promoting apoptosis and inhibiting growth in HT29 cells.
total:3
A non-canonical MEK/ERK signaling pathway regulates autophagy via regulating Beclin 1. 2009 to the paper
Abgent anti-PI3KC3 antibody was used in western blot in order to study the role of Beclin 1 in the regulation of autophagy. more
total:6
Protein kinase activity of phosphoinositide 3-kinase regulates cytokine-dependent cell survival. 2013 to the paper
Santa Cruz polyclonal anti-p110beta antibody was used to perform immunoprecipitation to study the role of phosphoinositide 3-kinase in the regulation of cytokine-dependent cell survival more
total:11
Phosphatidylinositol-4-phosphate 5-kinase 1alpha mediates extracellular calcium-induced keratinocyte differentiation. 2009 to the paper
Millipore polyclonal anti-human p85 antibody was used to carry out western blot analysis in order to investigate the role of PIP5K1alpha in extracellular calcium induced generation of the second messengers IP3 and keratinocyte differentiation. more
total:1
Amino acids activate mTOR complex 1 via Ca2+/CaM signaling to hVps34. 2008 to the paper
Abgent rabbit anti-hVps15 antibody was used in western blot to identify the pathway and mechanism by which AAs signal to hVps34 to stimulate mTOR complex1 signaling.
total:15
Signaling kinase AMPK activates stress-promoted transcription via histone H2B phosphorylation. 2010 to the paper
Cell Signaling Technologies anti-PRKAA1 antibody was used to carry out western blot analysis in order to investigate the mechanism for AMP-activated protein kinase regulating cellular responses to metabolic stress. more
total:4
Signaling kinase AMPK activates stress-promoted transcription via histone H2B phosphorylation. 2010 to the paper
R&D technologies anti-PRKAA2 antibody was used to carry out western blot analysis and ChIP assays in order to investigate the mechanism for AMP-activated protein kinase regulating cellular responses to metabolic stress. more
total:1
The ancient drug salicylate directly activates AMP-activated protein kinase. 2012 to the paper
Abcam anti-AMPK-gamma1 was used to perform western blot in order to show that AMPK could be activated by salicylate directly.
total:8
mTORC1 senses lysosomal amino acids through an inside-out mechanism that requires the vacuolar H(+)-ATPase. 2011 to the paper
Cell Signaling anti-raptor was used to perform western blot in order to show that vacuolar H+-ATPase is involved in mTOR pathway for mTORC1 translocation and activation. more
total:2
Amino acids mediate mTOR/raptor signaling through activation of class 3 phosphatidylinositol 3OH-kinase. 2005 to the paper
Santa Cruz Biotechnology anti-Rheb antibody (C-19) was used in western blot to study amino acids mediate mTOR activation by signaling through class 3 PI3K, hVps34. more
total:36
p180 promotes the ribosome-independent localization of a subset of mRNA to the endoplasmic reticulum. 2012 to the paper
Cell Signalling anti-S6 (rabbit monoclonal) antibody was used to perform immunoblotting in order to study the effect of p180 on the ribosome-independent localization of a subset of mRNA to the endoplasmic reticulum. more
total:34
Autoamplification of Notch signaling in macrophages by TLR-induced and RBP-J-dependent induction of Jagged1. 2010 to the paper
Santa Cruz Biotechnology polyclonal anti-Shp2 antibody was used to perform western blot in order to study Notch signaling auto-amplification in macrophages which was regulated by TLR and RBP-J. more
total:6
Expression of Toll-like receptors in verruca and molluscum contagiosum. 2008 to the paper
Santa Cruz Biotechnology goat polyclonal anti-human TLR9 was used in immunohistochemistry to study the expression of toll-like receptors in verruca and molluscum contagiosum in cryostat sections from human skin. more
total:2
Rapid turnover of the mTOR complex 1 (mTORC1) repressor REDD1 and activation of mTORC1 signaling following inhibition of protein synthesis. 2008 to the paper
EMD Chemicals anti-TRB3 antibody was used to perform western blot in order to study the response of the mTORC1 signaling after the inhibition of protein synthesis. more
total:9
Thyroid hormone exerts negative feedback on hypothalamic type 4 melanocortin receptor expression. 2010 to the paper
Zymed Laboratories anti-TSC1 antibody was used to perform western blot and immunoprecipitation in order to study how carboxy terminal tail of polycystin-1 inhibit mTOR via increasing the interaction between TSC2 and TSC1. more
total:26
Thyroid hormone exerts negative feedback on hypothalamic type 4 melanocortin receptor expression. 2010 to the paper
Epitomics anti-TSC2 antibody was used to perform western blot and immunoprecipitation in order to study the mechanism by which carboxy terminal tail of polycystin-1 repress mTOR signaling via membrane retention of TSC2. more