G2/M Checkpoints
gene
reagent
sample publication
sample review
ATM
ATM serine/threonine kinase
total:50
The RSF1 histone-remodelling factor facilitates DNA double-strand break repair by recruiting centromeric and Fanconi Anaemia proteins. 2014 to the paper
Millipore anti-phospho-ATM-S1981 antibody was used to perform immunofluorescence assay in order to detect the requirment of RSF1 for the recruitment of CENPS/MHF1, CENPX/MHF2, FANCD2, and FANCI to IRIF. more
ATR
ATR serine/threonine kinase
total:24
The RSF1 histone-remodelling factor facilitates DNA double-strand break repair by recruiting centromeric and Fanconi Anaemia proteins. 2014 to the paper
Santa Cruz human ATR antibody was used to perform western blot assay in order to study the requirement of RSFI in DSB repair. more
ATRIP
ATR interacting protein
total:1
ATR and ATRIP are recruited to herpes simplex virus type 1 replication compartments even though ATR signaling is disabled. 2010 to the paper
R&D anti-ATRIP monoclonal antibody was used to perform immunofluorescence and western blot in order to study the interactions of ATR and ATRIP in gene expression and production of virus during HSV-1. infection.
CCNB2
cyclin B2
total:1
Distinct mammalian SWI/SNF chromatin remodeling complexes with opposing roles in cell-cycle control. 2007 to the paper
Santa Cruz Biotechnology cyclin B2 antibody was used in western blot to demonstrate the opposing roles of distinct mammalian SWI/SNF chromatin remodeling complexes in cell-cycle control.
Cdc2
cyclin dependent kinase 1
total:52
Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. 2010 to the paper
Santa Cruz anti-human Cdk1 antibody was used to perform western blot in order to study the role of cdc6 in chromosomal segregation mediated by cdk1 and separase. more
CDC25A
cell division cycle 25A
total:10
Death receptor-induced activation of the Chk2- and histone H2AX-associated DNA damage response pathways. 2009 to the paper
Santa Cruz Biotechnology anti-Cdc25A antibody was used in western blot in order to study the role of TRAIL for activation of DNA damage response pathways with histone H2AX and Chk2 phosphorylations. more
cdc25C
cell division cycle 25C
total:20
Chk2 is required for HSV-1 ICP0-mediated G2/M arrest and enhancement of virus growth.. 2008 to the paper
Epitomics anti-ATM antibody was used for western blot in order to investigate the role of Chk2 in the regulation of HSV-1 ICP0-mediated G2/M arrest and virus growth more
CDC6
cell division cycle 6
total:9
Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. 2010 to the paper
Santa Cruz anti-human Cdc6 antibody was used to perform immunoprecipitation in order to study the role of cdc6 in chromosomal segregation mediated by cdk1 and separase. more
CDC7
cell division cycle 7
total:1
Identification of Mcm2 phosphorylation sites by S-phase-regulating kinases. 2006 to the paper
Neomarker Cdc7 antibody was used in immunoprecipitation to isolate Cdc7 from HeLa cells and in western blot to examine Cdc7 levels in HeLa.
CDK2
cyclin dependent kinase 2
total:53
Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. 2010 to the paper
Santa Cruz anti-human Cdk2 antibody was used to perform western blot in order to study the role of cdc6 in chromosomal segregation mediated by cdk1 and separase. more
Chk1
checkpoint kinase 1
total:59
ATR and ATRIP are recruited to herpes simplex virus type 1 replication compartments even though ATR signaling is disabled. 2010 to the paper
Santa Cruz anti-Chk1 monoclonal antibody was used to perform western blot in order to study the interactions of ATR and ATRIP in gene expression and production of virus during HSV-1 infection. more
CHK2
checkpoint kinase 2
total:48
Chromosome segregation errors as a cause of DNA damage and structural chromosome aberrations. 2011 to the paper
Santa Cruz anti-chk2 was used to perform western blot and immunocytochemistry in order to show that chromosomal structural aberrations could be caused by chromosome segregation errors. more
CLSPN
claspin
total:6
Regulated degradation of FANCM in the Fanconi anemia pathway during mitosis. 2009 to the paper
Bethyl Laboratories rabbit anti-Claspin antibody was used to carry out western blot analysis in order to investigate the mechanism of FANCM degradation during mitosis. more
cyclin B1
total:64
Critical role of Pcid2 in B cell survival through the regulation of MAD2 expression. 2010 to the paper
Thermo Fisher Scientific anti-cyclin B1 monoclonal antibody was used to perform immunoblotting in order to study the critical effect of Pcid2 on B cell survival through the regulation of MAD2 expression. more
HUS1
HUS1 checkpoint clamp component
total:1
A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling. 2011 to the paper
Santa Cruz anti-Hus1 was used to perform western blot in order to show that RHINO is important for ATR activation during DNA damage responses.
MCM10
minichromosome maintenance 10 replication initiation factor
MCM2
minichromosome maintenance complex component 2
total:8
Identification of Mcm2 phosphorylation sites by S-phase-regulating kinases. 2006 to the paper
BD Biosciences antibody to total Mcm2 was used in western blot to examine the levels of total Mcm2 in HeLa cells. more
MCM3
minichromosome maintenance complex component 3
total:5
Orc1 controls centriole and centrosome copy number in human cells. 2009 to the paper
Santa Cruz Biotechnology rabbit pAb anti-MCM3 antibody was used for western blot in order to demonstrate that the copy numbers of centriole and centrosome were regulated by Orc1 in human cells. more
MCM4
minichromosome maintenance complex component 4
total:1
The MCM-binding protein ETG1 aids sister chromatid cohesion required for postreplicative homologous recombination repair. 2010 to the paper
Acbam anti-MCM4 antibody was used for western blot in order to investigate the role of the ETG1 in the regulation of endogenous DNA stress.
MCM5
minichromosome maintenance complex component 5
total:2
The DNA replication factor MCM5 is essential for Stat1-mediated transcriptional activation. 2005 to the paper
Bethyl Laboratories MCM5 antibody was used in ChIP assay to study the role of DNA replication factor MCM5 in Stat1-mediated transcriptional activation more
MCM6
minichromosome maintenance complex component 6
total:2
The MCM-binding protein ETG1 aids sister chromatid cohesion required for postreplicative homologous recombination repair. 2010 to the paper
Acbam anti-MCM6 antibody was used for western blot in order to investigate the role of ETG1 in the regulation of endogenous DNA stress. more
MCM7
minichromosome maintenance complex component 7
total:6
Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. 2010 to the paper
Santa Cruz anti-human Mcm7 antibody was used to perform western blot in order to study the role of cdc6 in chromosomal segregation mediated by cdk1 and separase. more
MCM8
minichromosome maintenance 8 homologous recombination repair factor
ORC1
origin recognition complex subunit 1
total:1
p53-Dependent regulation of Cdc6 protein stability controls cellular proliferation. 2005 to the paper
Abcam ORC1 antibody was used in western blot to investigate the regulation of Cdc6 protein stability by p53.
ORC2
origin recognition complex subunit 2
total:7
Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. 2010 to the paper
Santa Cruz anti-human Orc2 antibody was used to perform western blot in order to study the role of cdc6 in chromosomal segregation mediated by cdk1 and separase. more
ORC4
origin recognition complex subunit 4
total:1
Orc1 controls centriole and centrosome copy number in human cells. 2009 to the paper
Abcam goat pAb anti-Orc4 antibody was used for western blot in order to demonstrate that the copy numbers of centriole and centrosome were regulated by Orc1 in human cells.
PKMYT1
protein kinase, membrane associated tyrosine/threonine 1
RAD1
RAD1 checkpoint DNA exonuclease
total:2
A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling. 2011 to the paper
Novus Biologicals anti-Rad1 was used to perform western blot in order to show that RHINO is important for ATR activation during DNA damage responses. more
Rad17
RAD17 checkpoint clamp loader component
total:7
Interactions of human mismatch repair proteins MutSalpha and MutLalpha with proteins of the ATR-Chk1 pathway. 2010 to the paper
Bethyl Laboratories anti-Rad17 antibody was used for western blot in order to investigate the roles of MutSalpha and MutLalpha in the regulation of ATR-Chk1 pathway. more
Rad9
RAD9 checkpoint clamp component A
total:7
A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling. 2011 to the paper
Bethyl anti-Rad9 was used to perform western blot in order to show that RHINO is important for ATR activation during DNA damage responses. more
RPA2
replication protein A2
total:12
ATR and ATRIP are recruited to herpes simplex virus type 1 replication compartments even though ATR signaling is disabled. 2010 to the paper
GeneTex anti-RPA32 monoclonal antibody was used to perform immunofluorescence in order to study the interactions of ATR and ATRIP in gene expression and production of virus during HSV-1 infection. more
WEE1
WEE1 G2 checkpoint kinase
total:2
Regulated degradation of FANCM in the Fanconi anemia pathway during mitosis. 2009 to the paper
Santa Cruz Biotechnology rabbit anti-WEE1 antibody was used to carry out western blot analysis in order to investigate the mechanism of FANCM degradation during mitosis. more