G alpha (12/13) signalling events
gene
reagent
sample publication
sample review
ADRA1D
adrenoceptor alpha 1D
total:1
Circadian-related heteromerization of adrenergic and dopamine D? receptors modulates melatonin synthesis and release in the pineal gland. 2012 to the paper
Abcam rabbit anti-alpha1 receptor antibody was used to perform immunoprecipitation and immunostaining to study the modulation of the synthesis and release of the melatonin in the pineal gland by the heteromerization of adrenergic and dopamine D₄ receptors.
ARHGAP4
Rho GTPase activating protein 4
total:3
Carboxypeptidase E cytoplasmic tail-driven vesicle transport is key for activity-dependent secretion of peptide hormones. 2008 to the paper
Transduction Laboratories p115 antibody was used in western blot and immunocytochemistry in order to investigate the mechanism required for post-Golgi transport of POMC/ACTH vesicles to the plasma membrane for activity-dependent secretion more
ARHGEF7
Rho guanine nucleotide exchange factor 7
total:1
GM130-dependent control of Cdc42 activity at the Golgi regulates centrosome organization. 2009 to the paper
Santa Cruz Biotechnology anti-beta-PIX antibody was used in western blot to study the role for GM130-dependent control of Cdc42 activity at the golgi in regulating centrosome organization
cdc42
cell division cycle 42
total:36
Slit2 regulates attractive eosinophil and repulsive neutrophil chemotaxis through differential srGAP1 expression during lung inflammation. 2010 to the paper
Santa Cruz Biotechnology anti-Cdc42 antibody was used in western blot to reveal the effect of slit2 on eosinophil and neutrophil during lung inflammation. more
ECT2
epithelial cell transforming 2
total:2
CUX1 and E2F1 regulate coordinated expression of the mitotic complex genes Ect2, MgcRacGAP, and MKLP1 in S phase. 2009 to the paper
Santa Cruz Biotechnology anti-Ect2 antibody was used in western blot in order to study the reguation of the expression of Ect2, MgcRacGAP, and MKLP1 during cell cycle progression more
FGD1
FYVE, RhoGEF and PH domain containing 1
FGD2
FYVE, RhoGEF and PH domain containing 2
FGD4
FYVE, RhoGEF and PH domain containing 4
Galpha13
G protein subunit alpha 13
total:7
Cleavage of NIK by the API2-MALT1 fusion oncoprotein leads to noncanonical NF-kappaB activation. 2011 to the paper
Abcam anti-BAFF monoclonal antibody was used to perform western blot in order to study that cleveage of NIK leads to noncanonical NF- kB activation more
GNA12
G protein subunit alpha 12
total:6
G alpha12 interaction with alphaSNAP induces VE-cadherin localization at endothelial junctions and regulates barrier function. 2005 to the paper
Santa Cruz Biotechnology polyclonal anti-Galpha12 antibody (which recognizes N-terminal peptides of Galpha12 proteins) was used in western blot and immunocytochemistry to study the effects of interaction between G 12 and SNAP on VE-cadherin localization at endothelial junctions and barrier function. more
GNB5
G protein subunit beta 5
total:1
Localization and differential interaction of R7 RGS proteins with their membrane anchors R7BP and R9AP in neurons of vertebrate retina. 2007 to the paper
Dr. W. Simonds (NINDK) rabbit anti-VAChT antibody was used in western blot to study the localization and differential interaction of R7 RGS proteins with R7BP and R9AP in neurons of vertebrate retina.
GRF2
Ras protein specific guanine nucleotide releasing factor 2
MCF2
MCF.2 cell line derived transforming sequence
total:1
Nm23-H1 modulates the activity of the guanine exchange factor Dbl-1. 2008 to the paper
Santa Cruz rabbit anti-Dbl antibody was used to perform immunoprecipitation and western blot in order to show that Nm23-H1 negatively regulate cell migration and tumor metastasis by modulating the activity of Dbl1.
MCF2L
MCF.2 cell line derived transforming sequence like
NET1
neuroepithelial cell transforming 1
total:1
The dioxin receptor regulates the constitutive expression of the vav3 proto-oncogene and modulates cell shape and adhesion. 2009 to the paper
Santa Cruz Biotechnology anti-Net1 antibody was used to carry out western blot analysis in order to investigate the regulation of constitutive Vav3 expression along with cell plasticity and migration by dioxin receptor.
NGEF
neuronal guanine nucleotide exchange factor
OBSCN
obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
p110
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
total:26
Protein kinase activity of phosphoinositide 3-kinase regulates cytokine-dependent cell survival. 2013 to the paper
Cell Signalling anti-p110alpha was used to perform immunoprecipitation to the role of phosphoinositide 3-kinase in the regulation of cytokine-dependent cell survival more
p85
phosphoinositide-3-kinase regulatory subunit 1
total:43
Protein kinase activity of phosphoinositide 3-kinase regulates cytokine-dependent cell survival. 2013 to the paper
Upstate anti-p85 pAb was used to perform immunoprecipitation to the role of phosphoinositide 3-kinase in the regulation of cytokine-dependent cell survival more
PIK3R2
phosphoinositide-3-kinase regulatory subunit 2
total:11
Phosphatidylinositol-4-phosphate 5-kinase 1alpha mediates extracellular calcium-induced keratinocyte differentiation. 2009 to the paper
Millipore polyclonal anti-human p85 antibody was used to carry out western blot analysis in order to investigate the role of PIP5K1alpha in extracellular calcium induced generation of the second messengers IP3 and keratinocyte differentiation. more
PLEKHG2
pleckstrin homology and RhoGEF domain containing G2
PLEKHG5
pleckstrin homology and RhoGEF domain containing G5
Rac1
Rac family small GTPase 1
total:55
Rho GDP dissociation inhibitor 2 suppresses metastasis via unconventional regulation of RhoGTPases. 2009 to the paper
Millipore anti-Rac1 monoclonal antibody (1:1,000) was used to perform immunoblot in order to study the metastasis suppressed by Rho GDP dissociation inhibitor 2 (RhoGDI2) through Rho GTPases. more
RAC2
Rac family small GTPase 2
total:3
In vivo RNAi screening identifies regulators of actin dynamics as key determinants of lymphoma progression. 2009 to the paper
Proteintech Rac2 antibody was used to perform western blot in order to investigate the role of Rac2 in lymphoma progression more
RhoA
ras homolog family member A
total:49
Silencing of RhoA and RhoC expression by RNA interference suppresses human colorectal carcinoma growth in vivo. 2010 to the paper
Santa Cruz anti-RhoA antibody was used to perform immunohistochemistry in order to study the effect of silencing of RhoA and RhoC expression on human colorectal carcinoma growth in vivo. more
RhoB
ras homolog family member B
total:10
Glutamine deamidation and dysfunction of ubiquitin/NEDD8 induced by a bacterial effector family. 2010 to the paper
Santa Cruz Biotechnology anti-RhoB antibody was used to carry out western blot analysis in order to investigate the role for CHBP protein from Burkholderia pseudomallei as a potent inhibitor of the eukaryotic ubiquitination pathway. more
RhoC
ras homolog family member C
total:5
Silencing of RhoA and RhoC expression by RNA interference suppresses human colorectal carcinoma growth in vivo. 2010 to the paper
Santa Cruz anti-RhoC antibody was used to perform immunohistochemistry in order to study the effect of silencing of RhoA and RhoC expression on human colorectal carcinoma growth in vivo. more
ROCK1
Rho associated coiled-coil containing protein kinase 1
total:8
Distinct roles for ROCK1 and ROCK2 in the regulation of keratinocyte differentiation. 2009 to the paper
Santa Curz anit-ROCK1 antibody was used to perform western blot to study the different roles of ROCK1 and ROCK2 in regulating keratinocyte adhesion and terminal differentiation. more
ROCK2
Rho associated coiled-coil containing protein kinase 2
total:5
RhoE Is required for keratinocyte differentiation and stratification. 2009 to the paper
Santa Cruz Biotechnology rabbit polyclonal anti-ROCKII antibody (H-85) was used in western blot to study the role for RhoE in keratinocyte differentiation and stratification more
Sos
SOS Ras/Rac guanine nucleotide exchange factor 1
total:11
Abelson interacting protein 1 (Abi-1) is essential for dendrite morphogenesis and synapse formation. 2007 to the paper
Santa Cruz rabbit Sos-1 antibody was used in western blot to study the role of Abi-1 in morphogenesis and synapse formation. more
SOS2
SOS Ras/Rho guanine nucleotide exchange factor 2
total:1
The contribution of apoptosis-inducing factor, caspase-activated DNase, and inhibitor of caspase-activated DNase to the nuclear phenotype and DNA degradation during apoptosis. 2005 to the paper
BD Biosciences anti-SOS-2 antibody was used in western blot to study the role of AIF in nuclear phenotype and DNA degradation during apoptosis
Tiam1
TIAM Rac1 associated GEF 1
total:6
Rac1 GTPase and the Rac1 exchange factor Tiam1 associate with Wnt-responsive promoters to enhance beta-catenin/TCF-dependent transcription in colorectal cancer cells. 2008 to the paper
Santa Cruz Biotechnology Tiam1 antibody (C-16) was used to perform co-immunoprecipitation analyse and chromatin immunoprecipitation assays in order to demonstrate the mechanism between Rac1 and the canonical Wnt signalling pathway in colorectal cancer cells. more
TRIO
trio Rho guanine nucleotide exchange factor
VAV3
vav guanine nucleotide exchange factor 3
total:2
Vav3 oncogene activates estrogen receptor and its overexpression may be involved in human breast cancer. 2008 to the paper
Upstate Biotechnology anti Vav3 antibody was used in western blot and immunohistochemistry to study the activation of estrogen receptor by Vav3 oncogene and Vav3 overexpression in human breast cancer more
XPLN
Rho guanine nucleotide exchange factor 3