overview

Labome lists 605677 antibody products against 69381 genes, including 19175 human, 15900 mouse, and 14338 rat genes.

This is a summary of WB antibody products in Labome database. There are 121207 monoclonal WB antibody products, 279048 polyclonal WB antibody products, including WB antibodies against 19010 human genes.

If you are looking for WB antibodies against a specific gene, type in gene name in the box above and search. In the resultant gene webpage, select 'antibody' link for the correct gene to go to the antibody webpage, and use the WB filter there to obtain a list of WB antibodies for that gene.

about Western Blotting

Western blot (alternatively, immunoblot) is a proteomic technique used to detect specific proteins in cell or tissue lysates or extracts.

Immunoblotting can be used to determine the presence of specific protein, and its size/molecular weight (if the lysates or extracts are separated under denaturing condition). It can also be used to determine the relative abundance of a specific protein, although the absolute quantity can not be determined. In addition, if phospho antibody is used as the primary antibody, the phosphorylation status of a specific protein can be determined, usually after the protein has been immunoprecipitated.

A typical Western blot protocol is provided here for reference. Variations to this protocol exist, and can be found throughout the web. One important thing is that for each Western blot, it is alway a good idea or perhaps imperative to have both negative controls and positive controls. One of the most common controls (to ensure consistent sample loading) is to use beta-actin antibody blot after the Western bloting of primary antibodies and stripping. see commonly used beta-actin antibody.

Clonality

Labome product database lists 121207 monoclonal WB antibodies, 279048 polyclonal WB antibodies.

Gene Species

WB antibodies correspond to 19010 human genes, 15737 mouse genes, 14220 rat genes, 6048 cow genes, 5559 dog genes, 2722 chicken genes, 2360 zebrafish genes, 373 chimpanzee genes, 161 thale cress genes, 155 fruit fly genes, 109 Caenorhabditis elegans genes, 101 Rhesus monkey genes, 97 Saccharomyces cerevisiae S288c genes, 61 pig genes, 36 common platanna genes, 31 rabbit genes, 22 Schizosaccharomyces pombe 972h- genes, 16 Escherichia coli str. K-12 substr. MG1655 genes, 14 cat genes, 13 Borrelia burgdorferi B31 genes, 11 Aspergillus niger CBS 513.88 genes, 10 Kaposi's sarcoma-associated herpesvirus - Human herpesvirus 8 genes, 9 Mycobacterium tuberculosis H37Rv genes, 8 sheep genes, 7 Human immunodeficiency virus 1 genes, 7 western clawed frog genes, 6 Japanese rice genes, 6 equine genes, 5 SARS coronavirus genes, 5 Human herpesvirus 1 genes, 5 Plasmodium falciparum 3D7 genes, 4 Human herpesvirus 5 genes, 4 Cenibacterium arsenoxidans genes, 4 Atlantic salmon genes, 4 Chlamydomonas reinhardtii genes, 3 maize genes, 3 Candida albicans SC5314 genes, 3 red flour beetle genes, 3 Human adenovirus C genes, 3 European honey bee genes, 3 Hepatitis B virus genes, 3 rainbow trout genes, 3 Escherichia coli O157:H7 str. Sakai genes.

Host Species

In Labome product database, among WB antibodies, 213576 from rabbit host, 118675 from mouse host, 55458 from goat host, 4413 from sheep host, 4341 from rat host, 2763 from chicken host, 359 from hamsters host, 213 from donkey host, 208 from human host, 182 from guinea pigs host, 26 from golden hamster host, 14 from equine host, 11 from Anas sp. host.

Antigen Modification

In Labome product database, among WB antibodies, 9715 products are against phosphorylated antigens, 504 products are against acetylated antigens, 5 products are against methylated antigens, 3 products are against unphosphorylated antigens.

Antibody Conjugation

In Labome product database, among WB antibodies, 3360 are conjugated with HRP, 1942 are conjugated with biotin, 716 are conjugated with DyLight488, 706 are conjugated with AP, 665 are conjugated with FITC, 176 are conjugated with PE, 61 are conjugated with DyLight549, 58 are conjugated with DyLight649, 56 are conjugated with APC, 47 are conjugated with BMP, 25 are conjugated with DyLight680, 20 are conjugated with IRDye 800, 19 are conjugated with TRITC, 18 are conjugated with IRDye 700DX, 18 are conjugated with DyLight800, 15 are conjugated with Cy5, 13 are conjugated with IRDye 800CW, 12 are conjugated with AF488, 8 are conjugated with Cy3, 8 are conjugated with PerCP, 5 are conjugated with Cy5.5, 4 are conjugated with texas red, 4 are conjugated with AMCA, 3 are conjugated with ATTO 647N, 2 are conjugated with AF647, 2 are conjugated with agarose.