overview

Labome lists 608210 antibody products against 63065 genes, including 19157 human, 15860 mouse, and 14205 rat genes.

This is a summary of WB antibody products in Labome database. There are 127035 monoclonal WB antibody products, 306091 polyclonal WB antibody products, including WB antibodies against 19081 human genes.

If you are looking for WB antibodies against a specific gene, type in gene name in the box above and search. In the resultant gene webpage, select 'antibody' link for the correct gene to go to the antibody webpage, and use the WB filter there to obtain a list of WB antibodies for that gene.

about Western Blotting

Western blot (alternatively, immunoblot) is a proteomic technique used to detect specific proteins in cell or tissue lysates or extracts.

Immunoblotting can be used to determine the presence of specific protein, and its size/molecular weight (if the lysates or extracts are separated under denaturing condition). It can also be used to determine the relative abundance of a specific protein, although the absolute quantity can not be determined. In addition, if phospho antibody is used as the primary antibody, the phosphorylation status of a specific protein can be determined, usually after the protein has been immunoprecipitated.

A typical Western blot protocol is provided here for reference. Variations to this protocol exist, and can be found throughout the web. One important thing is that for each Western blot, it is alway a good idea or perhaps imperative to have both negative controls and positive controls. One of the most common controls (to ensure consistent sample loading) is to use beta-actin antibody blot after the Western bloting of primary antibodies and stripping. see commonly used beta-actin antibody.

Clonality

Labome product database lists 127035 monoclonal WB antibodies, 306091 polyclonal WB antibodies.

Gene Species

WB antibodies correspond to 19081 human genes, 15773 mouse genes, 14131 rat genes, 3656 dog genes, 3642 cow genes, 1461 chicken genes, 753 zebrafish genes, 573 chimpanzee genes, 398 thale cress genes, 225 Rhesus monkey genes, 148 fruit fly genes, 104 Saccharomyces cerevisiae S288c genes, 95 pig genes, 87 Caenorhabditis elegans genes, 51 common platanna genes, 49 rabbit genes, 42 Chlamydomonas reinhardtii genes, 23 Auricularia delicata TFB-10046 SS5 genes, 22 Schizosaccharomyces pombe 972h- genes, 20 sheep genes, 19 Escherichia coli str. K-12 substr. MG1655 genes, 17 cat genes, 17 equine genes, 15 Synechocystis sp. PCC 6803 genes, 13 maize genes, 13 Japanese rice genes, 13 Borrelia burgdorferi B31 genes, 11 Domestic guinea pig genes, 11 Aspergillus niger CBS 513.88 genes, 10 Kaposi's sarcoma-associated herpesvirus - Human herpesvirus 8 genes, 9 Mycobacterium tuberculosis H37Rv genes, 7 Human immunodeficiency virus 1 genes, 7 Chinese hamster genes, 6 Human herpesvirus 1 genes, 6 black cottonwood genes, 6 SARS coronavirus genes, 6 western clawed frog genes, 5 Human herpesvirus 5 genes, 4 domesticated barley genes, 4 Orang-utan genes, 4 Plasmodium falciparum 3D7 genes, 4 Atlantic salmon genes, 4 Influenza A virus (A/Goose/Guangdong/1/96(H5N1)) genes, 3 Thermosynechococcus elongatus BP-1 genes, 3 Candida albicans SC5314 genes, 3 bread wheat genes, 3 Human adenovirus C genes, 3 Staphylococcus aureus subsp. aureus MRSA252 genes, 3 Ostreococcus tauri genes, 3 red flour beetle genes, 3 Streptomyces cattleya NRRL 8057 = DSM 46488 genes, 3 Pseudomonas aeruginosa PAO1 genes, 3 Influenza A virus (A/Puerto Rico/8/34(H1N1)) genes, 3 European honey bee genes, 3 rainbow trout genes, 3 goat genes, 3 Hepatitis B virus genes, 3 soybean genes, 3 Streptomyces coelicolor A3(2) genes.

Host Species

In Labome product database, among WB antibodies, 247478 from rabbit host, 120353 from mouse host, 53474 from goat host, 4813 from rat host, 4126 from sheep host, 1742 from chicken host, 360 from hamsters host, 302 from guinea pigs host, 212 from donkey host, 193 from human host, 35 from golden hamster host, 12 from equine host, 11 from Anas sp. host.

Antigen Modification

In Labome product database, among WB antibodies, 11019 products are against phosphorylated antigens, 495 products are against acetylated antigens, 5 products are against methylated antigens.

Antibody Conjugation

In Labome product database, among WB antibodies, 14957 are conjugated with HRP, 13162 are conjugated with FITC, 12312 are conjugated with AP, 12249 are conjugated with PE, 11852 are conjugated with APC, 1872 are conjugated with biotin, 759 are conjugated with DyLight488, 488 are conjugated with PerCP, 172 are conjugated with DyLight680, 99 are conjugated with ATTO 594, 61 are conjugated with DyLight549, 58 are conjugated with DyLight649, 51 are conjugated with BMP, 49 are conjugated with ATTO 655, 27 are conjugated with Cy5, 21 are conjugated with PE/Cy7, 21 are conjugated with PerCP/Cy5.5, 19 are conjugated with IRDye 800, 18 are conjugated with DyLight800, 18 are conjugated with TRITC, 18 are conjugated with IRDye 700DX, 13 are conjugated with IRDye 800CW, 12 are conjugated with AF488, 9 are conjugated with Cy3, 5 are conjugated with Cy5.5, 4 are conjugated with AF647, 4 are conjugated with AMCA, 3 are conjugated with ATTO 647N, 2 are conjugated with texas red, 2 are conjugated with agarose.