Labome lists 828357 antibody products against 79214 genes, including 19257 human, 16526 mouse, and 15720 rat genes.
This is a summary of WB antibody products in Labome database. There are 150528 monoclonal WB antibody products, 493703 polyclonal WB antibody products, 7 recombinant WB antibody products, including WB antibodies against 19165 human genes.
If you are looking for WB antibodies against a specific gene, type in gene name in the box above and search. In the resultant gene webpage, select 'antibody' link for the correct gene to go to the antibody webpage, and use the WB filter there to obtain a list of WB antibodies for that gene.
Western blot (alternatively, immunoblot) is a proteomic technique used to detect specific proteins in cell or tissue lysates or extracts.
Immunoblotting can be used to determine the presence of specific protein, and its size/molecular weight (if the lysates or extracts are separated under denaturing condition). It can also be used to determine the relative abundance of a specific protein, although the absolute quantity can not be determined. In addition, if phospho antibody is used as the primary antibody, the phosphorylation status of a specific protein can be determined, usually after the protein has been immunoprecipitated.
A typical Western blot protocol is provided here for reference. Variations to this protocol exist, and can be found throughout the web. One important thing is that for each Western blot, it is alway a good idea or perhaps imperative to have both negative controls and positive controls. One of the most common controls (to ensure consistent sample loading) is to use beta-actin antibody blot after the Western bloting of primary antibodies and stripping. see commonly used beta-actin antibody.
WB antibodies correspond to 19165 human genes, 16424 mouse genes, 15623 rat genes, 8309 cow genes, 8104 dog genes, 3508 zebrafish genes, 2689 chicken genes, 574 chimpanzee genes, 472 thale cress genes, 244 Rhesus monkey genes, 215 fruit fly genes, 209 pig genes, 149 Caenorhabditis elegans genes, 118 Saccharomyces cerevisiae S288c genes, 111 common platanna genes, 99 rabbit genes, 86 equine genes, 48 sheep genes, 46 Chinese hamster genes, 46 Chlamydomonas reinhardtii genes, 35 Escherichia coli str. K-12 substr. MG1655 genes, 32 cat genes, 31 Domestic guinea pig genes, 28 western clawed frog genes, 26 Schizosaccharomyces pombe 972h- genes, 19 Auricularia delicata TFB-10046 SS5 genes, 17 Japanese rice genes, 13 maize genes, 13 Borrelia burgdorferi B31 genes, 11 Aspergillus niger CBS 513.88 genes, 10 Mycobacterium tuberculosis H37Rv genes, 10 Kaposi's sarcoma-associated herpesvirus - Human herpesvirus 8 genes, 8 Human immunodeficiency virus 1 genes, 6 SARS coronavirus genes, 5 rainbow trout genes, 5 goat genes, 4 Atlantic salmon genes, 4 Plasmodium falciparum 3D7 genes, 4 Influenza A virus (A/Goose/Guangdong/1/96(H5N1)) genes, 4 Streptomyces coelicolor A3(2) genes, 4 domesticated barley genes, 4 Human adenovirus C genes, 4 Orang-utan genes, 4 soybean genes, 4 European honey bee genes, 3 Human papillomavirus type 16 genes, 3 medaka genes, 3 Thermosynechococcus elongatus BP-1 genes, 3 Dictyostelium discoideum AX4 genes, 3 Pseudomonas aeruginosa PAO1 genes, 3 red flour beetle genes, 3 Hepatitis B virus genes, 3 domestic silkworm genes, 3 white-tufted-ear marmoset genes, 3 Escherichia coli O157:H7 str. Sakai genes, 3 Ostreococcus tauri genes, 3 Human herpesvirus 1 genes.
In Labome product database, among WB antibodies, 408835 from rabbit host, 150164 from mouse host, 71018 from goat host, 5359 from rat host, 4025 from sheep host, 3395 from chicken host, 538 from guinea pigs host, 387 from donkey host, 293 from hamsters host, 162 from human host, 24 from golden hamster host, 16 from equine host.
In Labome product database, among WB antibodies, 33085 are conjugated with biotin, 27419 are conjugated with FITC, 25098 are conjugated with HRP, 21466 are conjugated with AP, 21263 are conjugated with PE, 20886 are conjugated with APC, 829 are conjugated with DyLight 550, 685 are conjugated with PerCP, 316 are conjugated with ATTO 488, 307 are conjugated with Atto 700, 173 are conjugated with TRITC, 138 are conjugated with texas red, 109 are conjugated with ATTO 594, 83 are conjugated with DyLight488, 60 are conjugated with DyLight549, 57 are conjugated with DyLight649, 55 are conjugated with ATTO 655, 52 are conjugated with BMP, 41 are conjugated with gold, 34 are conjugated with AF488, 25 are conjugated with DyLight680, 23 are conjugated with DyLight 650, 22 are conjugated with Cy3, 21 are conjugated with agarose, 21 are conjugated with DyLight800, 18 are conjugated with PE-Cy5, 18 are conjugated with IRDye 800, 17 are conjugated with IRDye 700DX, 17 are conjugated with PerCP/Cy5.5, 15 are conjugated with Alexa Fluor 555, 14 are conjugated with PE/Cy7, 14 are conjugated with Cy5, 12 are conjugated with IRDye 800CW, 8 are conjugated with Cy5.5, 8 are conjugated with efluor 660, 7 are conjugated with AF647, 6 are conjugated with Cy2, 4 are conjugated with PE-Cy5.5, 4 are conjugated with rhodamine, 4 are conjugated with AMCA, 3 are conjugated with oyster 550, 2 are conjugated with ATTO 647N, 2 are conjugated with APC/Cy7, 2 are conjugated with eFluor 570, 2 are conjugated with Alexa Fluor 594.