overview

Labome lists 744203 antibody products against 61938 genes, including 19181 human, 15962 mouse, and 14324 rat genes.

This is a summary of WB antibody products in Labome database. There are 139703 monoclonal WB antibody products, 432870 polyclonal WB antibody products, 17 recombinant WB antibody products, including WB antibodies against 19100 human genes.

If you are looking for WB antibodies against a specific gene, type in gene name in the box above and search. In the resultant gene webpage, select 'antibody' link for the correct gene to go to the antibody webpage, and use the WB filter there to obtain a list of WB antibodies for that gene.

about Western Blotting

Western blot (alternatively, immunoblot) is a proteomic technique used to detect specific proteins in cell or tissue lysates or extracts.

Immunoblotting can be used to determine the presence of specific protein, and its size/molecular weight (if the lysates or extracts are separated under denaturing condition). It can also be used to determine the relative abundance of a specific protein, although the absolute quantity can not be determined. In addition, if phospho antibody is used as the primary antibody, the phosphorylation status of a specific protein can be determined, usually after the protein has been immunoprecipitated.

A typical Western blot protocol is provided here for reference. Variations to this protocol exist, and can be found throughout the web. One important thing is that for each Western blot, it is alway a good idea or perhaps imperative to have both negative controls and positive controls. One of the most common controls (to ensure consistent sample loading) is to use beta-actin antibody blot after the Western bloting of primary antibodies and stripping. see commonly used beta-actin antibody.

Clonality

Labome product database lists 139703 monoclonal WB antibodies, 432870 polyclonal WB antibodies, 17 recombinant WB antibodies.

Gene Species

WB antibodies correspond to 19100 human genes, 15857 mouse genes, 14214 rat genes, 2760 dog genes, 2734 cow genes, 994 chicken genes, 763 zebrafish genes, 543 chimpanzee genes, 371 thale cress genes, 214 Rhesus monkey genes, 199 pig genes, 156 fruit fly genes, 111 Saccharomyces cerevisiae S288c genes, 110 Caenorhabditis elegans genes, 101 common platanna genes, 91 rabbit genes, 85 equine genes, 46 Chinese hamster genes, 45 sheep genes, 43 Chlamydomonas reinhardtii genes, 31 Escherichia coli str. K-12 substr. MG1655 genes, 31 cat genes, 29 Domestic guinea pig genes, 24 Schizosaccharomyces pombe 972h- genes, 19 Auricularia delicata TFB-10046 SS5 genes, 15 Synechocystis sp. PCC 6803 genes, 13 maize genes, 13 Borrelia burgdorferi B31 genes, 12 Japanese rice genes, 11 Mycobacterium tuberculosis H37Rv genes, 11 Aspergillus niger CBS 513.88 genes, 10 Kaposi's sarcoma-associated herpesvirus - Human herpesvirus 8 genes, 9 Escherichia coli str. K-12 substr. W3110 genes, 8 western clawed frog genes, 7 Human immunodeficiency virus 1 genes, 6 SARS coronavirus genes, 5 Orang-utan genes, 5 Human herpesvirus 1 genes, 5 goat genes, 4 Plasmodium falciparum 3D7 genes, 4 Influenza A virus (A/Goose/Guangdong/1/96(H5N1)) genes, 4 domesticated barley genes, 4 Escherichia coli CFT073 genes, 4 soybean genes, 4 Atlantic salmon genes, 3 Pseudomonas aeruginosa PAO1 genes, 3 bread wheat genes, 3 white-tufted-ear marmoset genes, 3 red flour beetle genes, 3 Streptomyces coelicolor A3(2) genes, 3 Thermosynechococcus elongatus BP-1 genes, 3 European honey bee genes, 3 Staphylococcus aureus subsp. aureus MRSA252 genes, 3 Human adenovirus C genes, 3 rainbow trout genes, 3 Streptomyces cattleya NRRL 8057 = DSM 46488 genes, 3 Hepatitis B virus genes, 3 Ostreococcus tauri genes.

Host Species

In Labome product database, among WB antibodies, 356421 from rabbit host, 134925 from mouse host, 67804 from goat host, 5383 from rat host, 4019 from sheep host, 2613 from chicken host, 516 from guinea pigs host, 383 from donkey host, 348 from hamsters host, 112 from human host, 29 from golden hamster host, 15 from equine host.

Antigen Modification

In Labome product database, among WB antibodies, 13502 products are against phosphorylated antigens, 583 products are against acetylated antigens, 40 products are against methylated antigens, 3 products are against unphosphorylated antigens.

Antibody Conjugation

In Labome product database, among WB antibodies, 27831 are conjugated with FITC, 25428 are conjugated with HRP, 21885 are conjugated with AP, 21813 are conjugated with PE, 21371 are conjugated with APC, 2666 are conjugated with biotin, 735 are conjugated with PerCP, 175 are conjugated with TRITC, 138 are conjugated with texas red, 109 are conjugated with ATTO 594, 84 are conjugated with DyLight488, 61 are conjugated with DyLight549, 58 are conjugated with DyLight649, 54 are conjugated with ATTO 655, 47 are conjugated with BMP, 41 are conjugated with gold, 26 are conjugated with DyLight680, 24 are conjugated with PerCP/Cy5.5, 23 are conjugated with PE-Cy5, 22 are conjugated with PE/Cy7, 22 are conjugated with DyLight800, 21 are conjugated with Cy3, 19 are conjugated with IRDye 800, 19 are conjugated with Cy5, 18 are conjugated with IRDye 700DX, 15 are conjugated with AF488, 13 are conjugated with IRDye 800CW, 10 are conjugated with agarose, 8 are conjugated with Cy2, 8 are conjugated with Cy5.5, 6 are conjugated with AF647, 4 are conjugated with PE-Cy5.5, 4 are conjugated with AMCA, 3 are conjugated with rhodamine, 2 are conjugated with ATTO 647N.