Labome lists 468066 antibody products against 56874 genes, including 18820 human, 15081 mouse, and 12979 rat genes.
This is a summary of western blot antibody products in Labome database. There are 94223 monoclonal western blot antibody products, 210186 polyclonal western blot antibody products, including western blot antibodies against 18547 human genes.
If you are looking for western blot antibodies against a specific gene, type in gene name in the box above and search. In the resultant gene webpage, select 'antibody' link for the correct gene to go to the antibody webpage, and use the western blot filter there to obtain a list of western blot antibodies for that gene.
Western blot (alternatively, immunoblot) is a proteomic technique used to detect specific proteins in cell or tissue lysates or extracts.
Immunoblotting can be used to determine the presence of specific protein, and its size/molecular weight (if the lysates or extracts are separated under denaturing condition). It can also be used to determine the relative abundance of a specific protein, although the absolute quantity can not be determined. In addition, if phospho antibody is used as the primary antibody, the phosphorylation status of a specific protein can be determined, usually after the protein has been immunoprecipitated.
A typical Western blot protocol is provided here for reference. Variations to this protocol exist, and can be found throughout the web. One important thing is that for each Western blot, it is alway a good idea or perhaps imperative to have both negative controls and positive controls. One of the most common controls (to ensure consistent sample loading) is to use beta-actin antibody blot after the Western bloting of primary antibodies and stripping. see commonly used beta-actin antibody.
Western blot antibodies correspond to 18547 human genes, 15143 mouse genes, 12949 rat genes, 3155 dog genes, 2810 cow genes, 1045 chicken genes, 859 zebrafish genes, 428 chimpanzee genes, 253 thale cress genes, 138 Saccharomyces cerevisiae S288c genes, 136 fruit fly genes, 97 pig genes, 84 Rhesus monkey genes, 83 Caenorhabditis elegans genes, 36 Chlamydomonas reinhardtii genes, 36 common platanna genes, 35 rabbit genes, 22 Schizosaccharomyces pombe 972h- genes, 20 cat genes, 15 sheep genes, 15 Escherichia coli str. K-12 substr. MG1655 genes, 13 Borrelia burgdorferi B31 genes, 11 maize genes, 11 Aspergillus niger CBS 513.88 genes, 9 Kaposi's sarcoma-associated herpesvirus - Human herpesvirus 8 genes, 9 equine genes, 8 Mycobacterium tuberculosis H37Rv genes, 7 western clawed frog genes, 6 Japanese rice genes, 5 spinach genes, 5 Synechococcus sp. PCC 7942 genes, 5 Human immunodeficiency virus 1 genes, 5 SARS coronavirus genes, 4 Human herpesvirus 1 genes, 3 European honey bee genes, 3 Human adenovirus C genes, 3 rainbow trout genes, 3 Escherichia coli CFT073 genes, 3 soybean genes, 3 Pantoea ananatis LMG 20103 genes, 3 Chlamydia trachomatis D/UW-3/CX genes, 3 Human herpesvirus 5 genes, 3 Influenza B virus genes, 3 Influenza A virus (A/Goose/Guangdong/1/96(H5N1)) genes, 3 Escherichia coli APEC O1 genes, 3 red flour beetle genes, 3 Hepatitis B virus genes.
In Labome product database, among western blot antibodies, 155522 from rabbit host, 86469 from mouse host, 49316 from goat host, 4214 from rat host, 3463 from sheep host, 2850 from human host, 1811 from chicken host, 366 from hamsters host, 170 from guinea pigs host, 167 from donkey host, 16 from golden hamster host, 15 from equine host, 15 from Anas sp. host.
In Labome product database, among western blot antibodies, 2907 are conjugated with HRP, 1968 are conjugated with biotin, 678 are conjugated with AP, 593 are conjugated with FITC, 478 are conjugated with DyLight488, 184 are conjugated with PE, 57 are conjugated with DyLight549, 54 are conjugated with DyLight649, 50 are conjugated with APC, 37 are conjugated with BMP, 21 are conjugated with IRDye 800, 20 are conjugated with Cy5, 19 are conjugated with IRDye 700DX, 18 are conjugated with DyLight800, 15 are conjugated with TRITC, 14 are conjugated with IRDye 800CW, 12 are conjugated with AF488, 12 are conjugated with DyLight680, 11 are conjugated with Cy3, 8 are conjugated with PerCP, 5 are conjugated with Cy5.5, 3 are conjugated with ATTO 647N, 2 are conjugated with AMCA, 2 are conjugated with AF647, 2 are conjugated with agarose, 2 are conjugated with texas red.