Labome/ExactAntigen
HA Hemagglutinin Tag Antibody
This is a review about hemagglutinin antibodies (HA antibodies), based on 23 pusblished articles using hemagglutinin antibodies in western blot, immunoprecipitation, ELISA, and other immunological technologies. There are numerous publications using hemagglutinin antibodies. The review is aimed to facilitate Labome visitors to locate the most suited hemagglutinin antibodies. Hemagglutinin antibodies from different suppliers can be searched here.

It is common to include a small segment of the viral hemagglutinin coat protein in gene expression vectors as an epitope tag. Such a tag can be easily recognized by hemagglutinin antibodies, and the expressed protein can then be purified or characterized.

Sigma Aldrich search Sigma Aldrich HA antibody
Zhao, Weihan et al. used anti-HA epitope tag antibody (Sigma H9658) to immunoprecipitate CMP-sialic acid transporter (CST) proteins from cell lysates to investigate its localization in the medial-trans Golgi and its two specific endoplasmic reticulum export motifs in carboxyl-terminal cytoplasmic tail in 2006. In 2008, David Allard et al. used the antibody to study Akt regulation of the survival of vascular smooth muscle cells via inhibition of FoxO3a and GSK3 and Olivier van Beekum et al. used to identify the HIV-1 Tat-interacting protein 60 (Tip60) as a novel positive regulator of PPAR gamma.

Santa Cruz Biotechnology search Santa Cruz Biotechnolog HA antibody
Estelle Nicolas et al. in 2003 used Santa-Cruz Biotechnology anti-HA antibody (Y-11) in immunoprecipitation to study the acetylation and methylation of histone H3 lysine 9 on the E2F-responsive dihydrofolate reductase promoter. Katharine Herrick-Davis et al. in 2005 used anti-HA (Y-11) antibody in immunoprecipitation to study the inhibition of serotonin 5-hydroxytryptamine2c receptor function through heterodimerization. Xuehui Yang et al.in 2004 used Santa Cruz Biotechnology anti-HA antibody in western blot to study the interaction of Sef with TAK1.

Roche
Yuko Fukata et al. in 2006 used Roche anti-HA antibody to immunoprecipitate cloned KSHV ORF proteins. In 2005, Martijn P Lolkema et al. used mouse clone 12CA5 in immunoprecipitation to study tumor suppression by the von Hippel-Lindau protein and Erik W Wilker et al. in 2005 used the same clone in western bolt to study the structural basis for 14-3-3 sigma functional specificity. Kyeong-Man Kim et al. in 2005 used anti-HA antibody in western blot and immunoprecipitation to study dopamine D3 receptor signaling and Karen L Wu et al. in 2004 used it in western blot and immunoprecipitation to study Akt-dependent cell survival through the regulation of ezrin/radixin/moesin proteins. Sinead M Miggin et al. in 2003 used rat monoclonal anti-hemagglutinin (HA) peroxidase-conjugated antibody (3F10) in western blot to study the palmitoylation of the human prostacyclin receptor. Jianguo Xu et al. in 2003 used clone 12CA5 in western blot and immunocytochemistry to study the heterodimerization of alpha-2A- and beta-1-adrenergic receptors. K Sauer et al.in 2001 used clone 3F10 in western blot and immunoprecipitation to study interaction of HPK1 with BLNK in B cells and with SLP-76 in T cells. Roche anti-HA antibody was used in western blot and immunoprecipitations to study Myc represses transcription through recruitment of DNA methyltransferase corepressor by Dr. Brenner in 2005. Roche Applied Science rat monoclonal anti-HA antibody was used in immunohistochemistry and immunocytochemistry to study the mechanism that dominant negative Rab3D mutants reduce GTP-bound endogenous Rab3D in pancreatic acini from the male ICR mice by Dr. Chen in 2003.

BabCO/Covance Research Products
Sinead M Miggin et al. in 2003 used BabCO mouse monoclonal anti-HA antibody (101R) in immunoprecipitation to study the palmitoylation of the human prostacyclin receptor. Sylvia N Schreiber et al.in 2003 used HA.11 antibody in western blot to investigate the expression and activity regulation of the orphan nuclear receptor estrogen-related receptor-alpha (ERR-alpha) by the transcriptional coactivator PGC-1. Estelle Nicolas et al. in 2003 used 16B12 antibody in western blot and immunoprecipitation to study the acetylation and effect of histone H3 lysine 9 methylation on the E2F-responsive dihydrofolate reductase promoter. Elena Díaz-Rodríguez et al. in 2002 used anti-hemagglutinin (HA) monoclonal antibody in immunoprecipitation and western blot to study the effect of a dominant negative form of Erk2 on PMA-induced TrkA cleavage. And Covance Research Products anti-HA antibody was used in western blot and immunoprecipitation to prove human HTm4 is a hematopoietic cell cycle regulator in 2002. BAbCo mouse monoclonal HA antibody was used in immunoprecipitation to study the role of Vma21p in assembly and transport of the yeast vacuolar ATPase the potential to utilize this cell line for structure/function studies of PDE6 by Dr. Malkus in 2004.

Cliniscience
Cliniscience rabbit anti-HA antibody SG77 was used in ELISA to study uncoupling and endocytosis of 5-hydroxytryptamine 4 receptors in 2005.

Boehringer Mannheim
Boehringer Mannheim HA antibody was used in emsa to demonstrate that meis homeoproteins directly regulate Pax6 during vertebrate lens morphogenesis by Dr..Zhang in 2002.

Articles reviewed

Allard, David et al. Akt regulates the survival of vascular smooth muscle cells via inhibition of FoxO3a and GSK3. 2008.
Barthet, Gaël et al. Uncoupling and endocytosis of 5-hydroxytryptamine 4 receptors. Distinct molecular events with different GRK2 requirements. 2005.
Brenner, Carmenet al.Myc represses transcription through recruitment of DNA methyltransferase corepressor. 2005
Chen, Xuequnet al.Dominant negative Rab3D mutants reduce GTP-bound endogenous Rab3D in pancreatic acini. 2003
Díaz-Rodríguez, Elena et al. Extracellular signal-regulated kinase phosphorylates tumor necrosis factor alpha-converting enzyme at threonine 735: a potential role in regulated shedding. 2002.
Donato, José L et al. Human HTm4 is a hematopoietic cell cycle regulator. 2002.
Fukata, Yuko et al. Epilepsy-related ligand/receptor complex LGI1 and ADAM22 regulate synaptic transmission. 2006.
Herrick-Davis, Katharine et al. Inhibition of serotonin 5-hydroxytryptamine2c receptor function through heterodimerization: receptor dimers bind two molecules of ligand and one G-protein. 2005.
Kim, Kyeong-Man et al. G protein-coupled receptor kinase regulates dopamine D3 receptor signaling by modulating the stability of a receptor-filamin-beta-arrestin complex. A case of autoreceptor regulation. 2005.
Lolkema, Martijn P et al. Tumor suppression by the von Hippel-Lindau protein requires phosphorylation of the acidic domain. 2005.
Malkus, Peret al.Role of Vma21p in assembly and transport of the yeast vacuolar ATPase. 2004
Miggin, Sinead M et al. Palmitoylation of the human prostacyclin receptor. Functional implications of palmitoylation and isoprenylation. 2003.
Nicolas, Estelle et al. Balance between acetylation and methylation of histone H3 lysine 9 on the E2F-responsive dihydrofolate reductase promoter. 2003.
Sauer, K et al. Hematopoietic progenitor kinase 1 associates physically and functionally with the adaptor proteins B cell linker protein and SLP-76 in lymphocytes. 2001.
Schreiber, Sylvia N et al. The transcriptional coactivator PGC-1 regulates the expression and activity of the orphan nuclear receptor estrogen-related receptor alpha (ERRalpha). 2003.
van Beekum, Olivier et al. The adipogenic acetyltransferase Tip60 targets activation function 1 of peroxisome proliferator-activated receptor gamma. 2008
Wilker, Erik W et al. A structural basis for 14-3-3sigma functional specificity. 2005.
Wu, Karen L et al. The NHE1 Na+/H+ exchanger recruits ezrin/radixin/moesin proteins to regulate Akt-dependent cell survival. 2004.
Xu, Jianguo et al. Heterodimerization of alpha 2A- and beta 1-adrenergic receptors. 2003.
Yang, Xuehui et al. Sef interacts with TAK1 and mediates JNK activation and apoptosis. 2004.
Zhang, Songwen et al. Interaction of p58(PITSLRE), a G2/M-specific protein kinase, with cyclinD3. 2002.
Zhang, Xinet al.Meis homeoproteins directly regulate Pax6 during vertebrate lens morphogenesis. 2002
Zhao, Weihan et al. The CMP-sialic acid transporter is localized in the medial-trans golgi and possesses two Specific endoplasmic reticulum export motifs in its carboxyl-terminal cytoplasmic tail. 2006
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