This is a review about Lep, based on 8 published articles (read how Labome selects the articles), using Lep antibody in all methods. It is aimed to help Labome visitors find the most suited Lep antibody.
Lep synonym: obese; obesity factor

Santa Cruz Biotechnology
rabbit polyclonal (H-146)
  • WB; human; 1:100-400
  • WB; rat; 1:100-400
In order to investigate apelin signaling in brown adipocyte tissue, Santa Cruz Biotechnology Lep antibody (Santa Cruz Biotechnology, sc-9014) was used in western blot on human samples at 1:100-400 and on rat samples at 1:100-400. J Biol Chem (2015) ncbi
rabbit polyclonal (H-146)
  • WB; human
Santa Cruz Biotechnology Lep antibody (Santa Cruz, SC-9014) was used in western blot on human samples. J Vis Exp (2014) ncbi
rabbit polyclonal (A-20)
  • WB; rat
  • IHC; rat
Santa Cruz Biotechnology Lep antibody (Santa Cruz Biotech., sc-842) was used in western blot on rat samples and in immunohistochemistry on rat samples. Biotech Histochem (2014) ncbi
R and D Systems
goat polyclonal
  • FC; mouse; fig s4
R and D Systems Lep antibody (R&D Systems, BAF497) was used in flow cytometry on mouse samples (fig s4). Stem Cell Reports (2015) ncbi
not indicated
  • ELISA; mouse
R&D systems leptin ELISA kit was used to perform ELISA in order to study the effect of adipose tissue lipolysis on glucose metabolism and insulin sensitivity.PLoS Biol (2013) ncbi
Invitrogen
rabbit polyclonal
  • IHC-P; sheep; 1:500
In order to measure FGF2, Ang1, VEGF, ObRb, and leptin expression in the corpus luteum of goats, Invitrogen Lep antibody (Thermo Scientific, PA1-052) was used in immunohistochemistry - paraffin section on sheep samples at 1:500. Anim Reprod Sci (2014) ncbi
EMD Millipore
not indicated
  • ELISA; mouse
Linco Research Inc. mouse serum leptin ELISA kit was used to quantify mouse serum leptin in order to study the effect of dietary curcumin on obesity-associated inflammation and diabetes in mouse models of diabesity.Endocrinology (2008) ncbi
Crystal Chem
not indicated
  • ELISA; mouse
Crystal Chem mouse anti-Leptin antibody (which is in a ELISA Kit) was used in ELISA to study the role for ghrelin receptors in the development of diet-induced obesity in mice.J Clin Invest (2005) ncbi
Articles Reviewed
  1. Sally James et al. (2015). "Multiparameter Analysis of Human Bone Marrow Stromal Cells Identifies Distinct Immunomodulatory and Differentiation-Competent Subtypes".PMID 26070611
  2. Aung Than et al. (2015). "Apelin Enhances Brown Adipogenesis and Browning of White Adipocytes".PMID 25931124
  3. Jessica R Wiles et al. (2014). "The effect of leptin on luteal angiogenic factors during the luteal phase of the estrous cycle in goats".PMID 24962614
  4. Melissa V Fernandez et al. (2014). "Activation and measurement of NLRP3 inflammasome activity using IL-1β in human monocyte-derived dendritic cells".PMID 24894187
  5. M Ates et al. (2014). "Anxiety- and depression-like behavior are correlated with leptin and leptin receptor expression in prefrontal cortex of streptozotocin-induced diabetic rats".PMID 24007473
  6. Amandine Girousse et al. (2013). "Partial inhibition of adipose tissue lipolysis improves glucose metabolism and insulin sensitivity without alteration of fat mass".PMID 23431266
  7. Stuart P Weisberg et al. (2008). "Dietary curcumin significantly improves obesity-associated inflammation and diabetes in mouse models of diabesity".PMID 18403477
  8. Jeffrey M Zigman et al. (2005). "Mice lacking ghrelin receptors resist the development of diet-induced obesity".PMID 16322794