This is a review about p65, based on 124 published articles (read how Labome selects the articles), using p65 antibody in all methods. It is aimed to help Labome visitors find the most suited p65 antibody.
p65 synonym: NFKB3; p65; NF-kappa-B p65delta3; nuclear factor NF-kappa-B p65 subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; transcription factor p65

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Santa Cruz BiotechnologySanta Cruz Biotechnology p65 product
  • ChIP assayIn 2010, Wei Li et al. employed Santa Cruz Biotechnology p65 antibody to perform ChIP assay in order to show that viral replication was inhibited by the elevated IL-32 levels during influenza virus infections [ncbi , more ]. Xiaoping Chen et al. applied Santa Cruz Biotechnology p65 antibody to demonstrate that RelB functions as a dual transcription regulator during endotoxin tolerance [ncbi , more ] in 2009 and I Paz-Priel et al. used Santa Cruz Biotechnology p65 antibody to carry out western blot analysis and CHIP assays in order to investigate the mechanism for oncoprotein C/EBPa cooperated with NF-kB p50 to inhibit apoptosis [ncbi , more ]. Santa Cruz Biotechnology p65 antibody was employed in 2008 to study the contribution of c-Jun to ccl2 transcriptional regulation in genetically modified mouse fibroblasts and in human cells (catalog: sc-372) [ncbi , more ], to study the role of NF-kappaB in transcriptional regulation of PIK3CA oncogene in ovarian cancer microenvironment [ncbi , more ], to demonstrate that CBP dosage is a critical regulator of gene-specific signal integration between the ER- and NFkappaB-signaling pathways [ncbi , more ]and to identify NF-kappaB target genes involved in thyroid cancer [ncbi , more ]. In 2007, Zhiqiang Zhang et al. used Santa Cruz Biotechnology p65 antibody to study the role of transcription termination factor Pcf11 on the processivity of Pol II [ncbi , more ]. Santa Cruz Biotechnology p65 antibody was utilized in 2006 to study the effects of oxygen on cyclic AMP and interleukin-1 stimulation of surfactant protein A gene expression [ncbi , more ], to study p65 in the human astrocytoma cell line 1321N1 (catalog: sc-372) [ncbi , more ]and to study the role of IPS-1, IRF3, and IFN in Legionella infection of lung epithelium in human A549 cells [ncbi , more ]. Santa Cruz Biotechnology p65 antibody was utilized in 2005 to detect NF-kappaB DNA binding activity and in ChIP assay to determine whether p65/NF-kappaB binds to the B site identified in the Plk3 promoter using MDAPanc-28/Puro and MDAPanc28/IkappaBalphaM human tumor cell lines [ncbi , more ], to study the biological function of Peroxisome Proliferator-activated Receptor gamma (PPARgamma) in the regulation of chemokine expression [ncbi , more ]and to investigate the IL-8 and IkappaBalpha gene regulatory regions in A549 human lung carcinoma cells (catalog: sc-109) [ncbi , more ].
  • EMSAIn 2009, Derek S Wheeler et al. used Santa Cruz Biotechnology p65 antibody to demonstrate whether Hsp72 is released from virally infected airway epithelial cells resulting in the recruitment and activation of neutrophils [ncbi , more ]. Santa Cruz Biotechnology p65 antibody was employed in 2008 to demonstrate that the effects of vitamin E on cell growth parameters do not solely through decreased NF-kappaB activation [ncbi , more ], to study the roles for mitogen-activated protein kinases and NFkappaB in LPS-induced CD40 expression on human monocytic cells [ncbi , more ], to study CXCL10 gene expression and regulation by the pro-inflammatory cytokines interleukin (IL) 1beta, interferon (IFN) gamma, and tumour necrosis factor (TNF) alpha in intestinal epithelial cell lines [ncbi , more ]and to teste the hypothesis that atorvastatin may be capable of inducing apoptosis in HSCs [ncbi , more ]. M V P Nadella et al. used Santa Cruz Biotechnology p65 antibody to perform EMSA and western blot in order to study the transcriptional regulation of PTHrP in ATLL cells by NF-kB (catalog: sc-109) [ncbi , more ] in 2007 and Yutaka Kida et al. used Santa Cruz Biotechnology p65 antibody to study the role for protease-activated receptor 2 in serratia marcescens serralysin induced inflammatory responses in human cell lines [ncbi , more ]. Santa Cruz Biotechnology p65 antibody was employed in 2006 to study the role for protein methyltransferase 2 in inhibiting NF-kappaB function and promoting apoptosis in human kidney cell line 293 [ncbi , more ], to study the role for IL-1 receptor-associated kinase 1 in latent membrane protein 1-induced p65/RelA serine 536 phosphorylation and NF-kappaB activation in human I1A 293 cells [ncbi , more ], to study p65 in the human astrocytoma cell line 1321N1 (catalog: sc-372) [ncbi , more ], to detect p65 in TAK1+/+ and TAK1/ MEFs transfected with expression vectors for Flag-LMP1 [ncbi , more ]and to study the role for the effect of human papillomavirus Type 16 E6 on NF-kappaB activation and cIAP-2 expression in protecting from apoptosis in primary human airway epithelial cells [ncbi , more ]. Zhongkui Li et al. utilized Santa Cruz Biotechnology p65 antibody to detect NF-kappaB DNA binding activity and in ChIP assay to determine whether p65/NF-kappaB binds to the B site identified in the Plk3 promoter using MDAPanc-28/Puro and MDAPanc28/IkappaBalphaM human tumor cell lines [ncbi , more ] in 2005 and Chaitali Basak et al. used Santa Cruz Biotechnology p65 antibody to evaluate the role of NF-kappa B- and C/EBP beta-driven interleukin-1 beta gene expression and PAK1-mediated caspase-1 activation in interleukin-1 beta release from Helicobacter pylori lipopolysaccharide-stimulated macrophages [ncbi , more ]. Santa Cruz Biotechnology p65 antibody was used in 2004 to study the effect of multiple protein kinases and an unknown kinase and couples p65 to TATA-binding protein-associated factor II31-mediated interleukin-8 transcription on constitutive and interleukin-1-inducible phosphorylation of p65 NF- B at serine 536 [ncbi , more ], to study the NF- B activation, uPA up-regulation, and hepatitis B viral X protein (HBx) expression in human hepatocellular carcinoma (HCC) and their clinicopathological significance [ncbi , more ], to study the regulation of human 3' Igh hs4 enhancer activity in B cells by synergistic interaction between Oct-2 and NF-kappaB [ncbi , more ]and to study translational repression mediating activation of nuclear factor kappa B by phosphorylated translation initiation factor 2 [ncbi , more ]. Santa Cruz Biotechnology p65 antibody was applied in 2003 to study the role of tyrosine kinase p56lck in the regulation of cell motility and nuclear factor kappaB-mediated secretion of urokinase type plasminogen activator through tyrosine phosphorylation of IkappaBalpha following hypoxia/reoxygenation [ncbi , more ], to study the role for estrogen withdrawal-induced NF-kappaB activity and Bcl-3 expression in growth and hormone independence in human breast cancer cells [ncbi , more ], to study the effect of upregulation of HMG1 on melanoma inhibitory activity expression in malignant melanoma cells [ncbi , more ], to study the Stat3 expression, localization, and activity in alcoholic liver disease (ALD) in humans [ncbi , more ], to study RANTES expression regulation by plasma membrane CFTR [ncbi , more ]and to study the roles for basic fibroblast growth factor and fenofibric acid in induction and modulation of tissue factor expression in human endothelial cells [ncbi , more ]. Santa Cruz Biotechnology p65 antibody was used in 2002 to study the effect of transforming growth factor- 1 (TGF- 1) on human type II collagen gene expression in articular chondrocytes and the relationship of it with Sp3/Sp1 ratio [ncbi , more ], to study the role of NF-kappaB in prostate cancer progression in human cells (catalog: SC-372-G) [ncbi , more ], to study the stimulation of IRF-7 expression by 12-O-tetradecanoylphorbol-13-acetate (TPA) and tumor necrosis factor alpha (TNFalpha) in human peripheral blood monocytes [ncbi , more ]and to study the roles for AP-1 and C/EBPbeta in the regulation of inducible granulysin gene expression in a human monocytic cell line THP-1 [ncbi , more ]. T Hideshima et al. used Santa Cruz Biotechnology p65 antibody to demonstrate the proteasome inhibitor PS-341 inhibiting growth, inducing apoptsis and overcoming drug resistance in human multiple myeloma cells [ncbi , more ] in 2001 and P C Lucas et al. used Santa Cruz Biotechnology p65 antibody to study the roles of Bcl10 and MALT1 in a novel NF- B signaling pathway [ncbi , more ].
  • immunocytochemistryAnna Vossenkamper et al. applied Santa Cruz Biotechnology p65 antibody to perform western blot and immunocytochemistry in order to study the role of the EPEC protein NleE in inhibiting NF-kappa B signaling in human dendritic cells [ncbi , more ] in 2010 and Julien Cherfils-Vicini et al. used Santa Cruz Biotechnology p65 antibody to perform immunofluorescence in order to study how TLR7 and TLR8 expressed by human lung cancer cells induced cell survival and chemoresistance [ncbi , more ]. Santa Cruz Biotechnology p65 antibody was employed in 2008 to perform immunocytochemistry in order to study the activation of NF-kappa B in correlation with RET mutational status (catalog: sc-372) [ncbi , more ], to investigate the effects of Inmunoferon-active principle (AM3) on human monocyte-derived DCs (MDDCs) [ncbi , more ]and to demonstrate that west nile virus nonstructural protein 1 inhibits TLR3 signal transduction [ncbi , more ]. Jinju Kim et al. applied Santa Cruz Biotechnology p65 antibody to determine whether Moutan Cortex Radicis (MCR) effects eotaxin secretion from A549 epithelial cells and eosinophil chemotaxis [ncbi , more ] in 2007 and Pabbisetty Sudheer Kumar et al. used Santa Cruz Biotechnology p65 antibody to study the role for p21cip/waf1 and p27kip1 in TNF-alpha-induced inhibition of proliferation in human glioma cells [ncbi , more ]. In 2006, Bryan D Griffin et al. employed Santa Cruz Biotechnology p65 antibody to study p65 in the human astrocytoma cell line 1321N1 (catalog: sc-372) [ncbi , more ]. In 2005, Hitoshi Ogawa et al. used Santa Cruz Biotechnology p65 antibody to study the mechanisms of MAdCAM-1 gene expression in human intestinal microvascular endothelial cells [ncbi , more ]. Ganapati H Mahabeleshwar et al. utilized Santa Cruz Biotechnology p65 antibody to study the role of tyrosine kinase p56lck in the regulation of cell motility and nuclear factor kappaB-mediated secretion of urokinase type plasminogen activator through tyrosine phosphorylation of IkappaBalpha following hypoxia/reoxygenation [ncbi , more ] in 2003 and Rosemary Kiernan et al. used Santa Cruz Biotechnology p65 antibody to detect p65 in Jurkat cells and HeLa cells [ncbi , more ].
  • immunohistochemistryIn 2009, Annika Lehmann et al. used Santa Cruz Biotechnology p65 antibody to perform immunohistochemistry in order to study the association between the high class I HDAC activity and RelA/p65 activation in pancreatic cancer [ncbi , more ]. Susan Nozell et al. used Santa Cruz Biotechnology p65 antibody to perform western blot and immunohistochemistry in order to study the association between ING4 and the activity of NF-kappa B molecules bound with target gene promoters [ncbi , more ] in 2008 and Nuo Yang et al. used Santa Cruz Biotechnology p65 antibody to study the role of NF-kappaB in transcriptional regulation of PIK3CA oncogene in ovarian cancer microenvironment [ncbi , more ]. In 2005, Sewon Kang et al. utilized Santa Cruz Biotechnology p65 antibody to study the role for activated transcription factors nuclear factor-kappaB and activator protein-1 in inflammation and extracellular matrix degradation in inflammatory acne lesions in vivo in skin cryosections from patients [ncbi , more ]. In 2004, Susan E McNulty et al. applied Santa Cruz Biotechnology p65 antibody to study the expression pattern of NFkappaB family members in human melanocytic cells of normal skin, benign intradermal naevus, and metastatic melanoma tissues [ncbi , more ]. In 2003, Christian Maihofner et al. applied Santa Cruz Biotechnology p65 antibody to study cyclooxygenase-2, interleukin-1beta, interleukin-6, and NF-kappaB expression in human colorectal cancer [ncbi , more ].
  • immunoprecipitationSerena Ghisletti et al. used Santa Cruz Biotechnology p65 antibody to investigate the role of NCoR/SMRT interactions in the regulation of inflammatory and anti-inflammatory signals in immunity and homeostasis [ncbi , more ] in 2009 and Bo Huang et al. used Santa Cruz Biotechnology p65 antibody to introduce Brd4 as a coactivator of NF-kappa B via specific binding to acetylated RelA [ncbi , more ]. In 2008, Erika Mathes et al. utilized Santa Cruz Biotechnology p65 antibody to demonstrate that NF-kappaB dictates the degradation pathway of IkappaBalpha (catalog: sc-372-G) [ncbi , more ]. In 2007, Hittu Matta et al. utilized Santa Cruz Biotechnology p65 antibody to study the K13-induced NF-B and JNK activation and re-examine the role of TRAFs in these processes [ncbi , more ]. Santa Cruz Biotechnology p65 antibody was used in 2006 to study the role of BRMS1 in the metastasis of breast cancer [ncbi , more ], to study the role for protein methyltransferase 2 in inhibiting NF-kappaB function and promoting apoptosis in human kidney cell line 293 [ncbi , more ], to study p65 in the human astrocytoma cell line 1321N1 (catalog: sc-372) [ncbi , more ]and to demonstrate that SSAT2 functions as a transcriptional coactivator for NF-kappaB and cooperates with CBP and P/CAF to enhance TNFalpha-induced NF-kappaB activity [ncbi , more ]. In 2005, Shashirekha Shetty et al. applied Santa Cruz Biotechnology p65 antibody to investigate the regulation of death receptor 5 expression by NFKB1 [ncbi , more ]. Mary H Ritchie et al. applied Santa Cruz Biotechnology p65 antibody to study the role for NF-kappaB binding motifs in the differential induction of chemokine gene expression in human corneal epithelial cells [ncbi , more ] in 2004 and Hyun Duk Jang et al. used Santa Cruz Biotechnology p65 antibody to study the effect of the interaction between PIAS3 and p65/RelA subunit on NF- B-mediated transcription in human 293T cells [ncbi , more ]. Ina Poser et al. employed Santa Cruz Biotechnology p65 antibody to study the effect of upregulation of HMG1 on melanoma inhibitory activity expression in malignant melanoma cells [ncbi , more ] in 2003 and Rosemary Kiernan et al. used Santa Cruz Biotechnology p65 antibody to detect p65 in Jurkat cells and HeLa cells [ncbi , more ].
  • western blotSanta Cruz Biotechnology p65 antibody was used in 2010 to carry out western blot analysis in order to investigate the role for CHBP protein from Burkholderia pseudomallei as a potent inhibitor of the (catalog: sc-372) [ncbi , more ], to carry out western blotting analysis in order to investigate the role of TNF-a in irradiation-induced bone marrow cell apoptosis and bone marrow dysfunction [ncbi , more ]and to perform western blot and immunocytochemistry in order to study the role of the EPEC protein NleE in inhibiting NF-kappa B signaling in human dendritic cells [ncbi , more ]. Santa Cruz Biotechnology p65 antibody was applied in 2009 to perform western blot in order to prove that the catalytic activity of IKK modified with O-GlcNAc in p53-deficient cells is enhanced (catalog: sc-372) [ncbi , more ], to study the role of MSK1 in NF-kB activation and inflammatory diseases [ncbi , more ], to demonstrate that the high expression of BCL3 at the time of diagnosis is associated with inferior prognosis (catalog: sc-109) [ncbi , more ], to demonstrate that Lyn can inhibit osteoclast formation [ncbi , more ], to introduce Brd4 as a coactivator of NF-kappa B via specific binding to acetylated RelA [ncbi , more ], to investigate the role of PARP-1 in the NF-kB signalling cascade induced by ionizing radiation [ncbi , more ], to carry out western blot analysis and CHIP assays in order to investigate the mechanism for oncoprotein C/EBPa cooperated with NF-kB p50 to inhibit apoptosis [ncbi , more ]and to perform western blot in order to show that the loss of Faf1 resulted in aberrant NF-kB signaling and tumor progression [ncbi , more ]. Santa Cruz Biotechnology p65 antibody was applied in 2008 to study the contribution of c-Jun to ccl2 transcriptional regulation in genetically modified mouse fibroblasts and in human cells (catalog: sc-372) [ncbi , more ], to perform western blot in order to study how genistein regulate tumor suppressor genes in prostate cancer cells [ncbi , more ], to investigate the effects of extracellular HSP70 on the production of RA-associated cytokines in fibroblast-like synoviocytes from patients with RA [ncbi , more ], to teste the hypothesis that atorvastatin may be capable of inducing apoptosis in HSCs [ncbi , more ]and to perform western blot and immunohistochemistry in order to study the association between ING4 and the activity of NF-kappa B molecules bound with target gene promoters [ncbi , more ]. Santa Cruz Biotechnology p65 antibody was used in 2007 to show that methylglyoxal inhibits TNF-induced NF-kappaB DNA binding and NF-kappaB-dependent reporter gene expression in a concentration-dependent manner [ncbi , more ], to study the role of transcription termination factor Pcf11 on the processivity of Pol II [ncbi , more ], to perform EMSA and western blot in order to study the transcriptional regulation of PTHrP in ATLL cells by NF-kB (catalog: sc-109) [ncbi , more ], to study the effect of the opioid antagonist, beta-funaltrexamine on chemokine expression in human astroglial cells [ncbi , more ], to demonstrate that CSN controls NF-kappaB by deubiquitinylation of IkappaBalpha [ncbi , more ], to demonstrate that p38 MAP kinase activity regulates the transcriptional activation of NF-kappaB in response to spirochetal lysate stimulation of phagocytic cells [ncbi , more ]and to determine whether Moutan Cortex Radicis (MCR) effects eotaxin secretion from A549 epithelial cells and eosinophil chemotaxis [ncbi , more ]. Santa Cruz Biotechnology p65 antibody was used in 2006 to study the role of BRMS1 in the metastasis of breast cancer [ncbi , more ], to study the role of IPS-1, IRF3, and IFN in Legionella infection of lung epithelium in human A549 cells [ncbi , more ], to study the role for protein methyltransferase 2 in inhibiting NF-kappaB function and promoting apoptosis in human kidney cell line 293 [ncbi , more ], to study the effects of oxygen on cyclic AMP and interleukin-1 stimulation of surfactant protein A gene expression [ncbi , more ], to study the role of the innate immune signaling molecule IRAK-4 in T cell activation [ncbi , more ], to study the role for IL-1 receptor-associated kinase 1 in latent membrane protein 1-induced p65/RelA serine 536 phosphorylation and NF-kappaB activation in human I1A 293 cells [ncbi , more ], to study p65 in the human astrocytoma cell line 1321N1 (catalog: sc-372) [ncbi , more ], to detect p65 in TAK1+/+ and TAK1/ MEFs transfected with expression vectors for Flag-LMP1 [ncbi , more ]and to demonstrate that SSAT2 functions as a transcriptional coactivator for NF-kappaB and cooperates with CBP and P/CAF to enhance TNFalpha-induced NF-kappaB activity [ncbi , more ]. Santa Cruz Biotechnology p65 antibody was employed in 2005 to investigate the decrease of COX-2 Levels through p53 and NFkB by T-oligo [ncbi , more ], to study the molecular mechanisms in enhancing the resistance to infectious microorganisms by disrupting the uncoupling protein-2 gene [ncbi , more ], to study the role for hTid-1 in the regulation of NF-kappaB activity and suppression of tumor growth [ncbi , more ]and to study the cellular and molecular function of a novel interferon- and LPS-inducible gene designated FLN29 that acts as a negative regulator in TLR signaling [ncbi , more ]. Santa Cruz Biotechnology p65 antibody was employed in 2004 to study the effect of multiple protein kinases and an unknown kinase and couples p65 to TATA-binding protein-associated factor II31-mediated interleukin-8 transcription on constitutive and interleukin-1-inducible phosphorylation of p65 NF- B at serine 536 [ncbi , more ], to detect p65 NF-kappaB in unstimulated and IL-1-treated HeLa cells incubated with a recombinant GST-p65- (354 551) fusion protein in the presence of [-32P]ATP [ncbi , more ], to detect p65 in human peripheral T lymphocytes [ncbi , more ], to elucidate that the p65/RelA degradation is highly associated with its stability and will promotes transcriptional termination [ncbi , more ], to study the role for NF-kappaB binding motifs in the differential induction of chemokine gene expression in human corneal epithelial cells [ncbi , more ], to study the NF- B activation, uPA up-regulation, and hepatitis B viral X protein (HBx) expression in human hepatocellular carcinoma (HCC) and their clinicopathological significance [ncbi , more ], to study the effect of the interaction between PIAS3 and p65/RelA subunit on NF- B-mediated transcription in human 293T cells [ncbi , more ], to study the identification and characterization of a novel ZU5 and death domain-containing protein designated ZUD in human 293 cells [ncbi , more ], to determine SAA3 in whole-cell homogenates induced by TNF [ncbi , more ], to study the regulation of human 3' Igh hs4 enhancer activity in B cells by synergistic interaction between Oct-2 and NF-kappaB [ncbi , more ], to study a role for STAG/Scc3 in transcriptional regulation by cohesins [ncbi , more ]and to detect RelA/p65 in HUVECs pretreated with Y-27632 [ncbi , more ]. Santa Cruz Biotechnology p65 antibody was utilized in 2003 to detect Rel A (p65) in RAW 264.7 cells [ncbi , more ], to study the role for estrogen withdrawal-induced NF-kappaB activity and Bcl-3 expression in growth and hormone independence in human breast cancer cells [ncbi , more ], to study the intracellular localization or functional domains of Par-4 involved in apoptosis [ncbi , more ], to study the role of RelB serine 368 for dimerization and p100 stabilization [ncbi , more ], to study the regulatory mechanisms for the phosphorylation of p65 on Ser-536 under physiological conditions [ncbi , more ], to study cyclooxygenase-2, interleukin-1beta, interleukin-6, and NF-kappaB expression in human colorectal cancer [ncbi , more ], to detect p65 in Jurkat cells and HeLa cells [ncbi , more ]and to study the role for bradykinin and interleukin-1beta in transcriptional regulation of cyclooxygenase 2 in human airway smooth muscle cells [ncbi , more ]. Santa Cruz Biotechnology p65 antibody was employed in 2002 to study the role of NF-kappaB in prostate cancer progression in human cells (catalog: SC-372-G) [ncbi , more ], to study the biological function of nerve growth factor in the regulation of dopamine D2 receptor expression [ncbi , more ]and to study the Rel family protein expression in splenic B cells of PKC /- (-/-) and PKC +/+ (+/+) mice [ncbi , more ]. In 2001, T Hideshima et al. used Santa Cruz Biotechnology p65 antibody to demonstrate the proteasome inhibitor PS-341 inhibiting growth, inducing apoptsis and overcoming drug resistance in human multiple myeloma cells [ncbi , more ].
Cell Signaling TechnologyCell Signaling Technology p65 product
  • ChIP assayIn 2003, Mei Nie et al. utilized Cell Signaling Technology p65 antibody to study the role for bradykinin and interleukin-1beta in transcriptional regulation of cyclooxygenase 2 in human airway smooth muscle cells [ncbi , more ].
  • EMSAIn 2010, Saira Ali et al. applied Cell Signaling Technology p65 antibody to carry out western blot analysis and EMSA assays in order to investigate the role for NF-kB signalling in the interleukin-1 induced expression of C/EBP delta [ncbi , more ].
  • immunocytochemistryIn 2004, Md Ruhul Abid et al. utilized Cell Signaling Technology p65 antibody to detect p65 NF-kappaB and phospho-p65 NF-kappaB in human coronary artery endothelial cells (HCAEC) [ncbi , more ].
  • immunohistochemistryIn 2003, Dirk Haller et al. used Cell Signaling Technology p65 antibody to study that transforming growth factor-beta 1 inhibits non-pathogenic Gram negative bacteria-induced NF-kappa B recruitment to the interleukin-6 gene promoter in intestinal epithelial cells through modulation of histone acetylation [ncbi , more ].
  • immunoprecipitationIn 2005, Dong Kee Lee et al. utilized Cell Signaling Technology p65 antibody to study the effect of on E-selectin transcription [ncbi , more ].
  • western blotJianhui Ma et al. applied Cell Signaling Technology p65 antibody to carry out western blot analysis in order to investigate the regulation of Notch signaling and cell differentiation by mTOR signaling [ncbi , more ] in 2010 and Saira Ali et al. used Cell Signaling Technology p65 antibody to carry out western blot analysis and EMSA assays in order to investigate the role for NF-kB signalling in the interleukin-1 induced expression of C/EBP delta [ncbi , more ]. Yun Song Lee et al. used Cell Signaling Technology p65 antibody to perform western blot in order to show that JNK1 can suppresses the expression of LPS-induced MMP-9 [ncbi , more ] in 2009 and Laurent Reber et al. used Cell Signaling Technology p65 antibody to study the role of MSK1 in NF-kB activation and inflammatory diseases [ncbi , more ]. Cell Signaling Technology p65 antibody was used in 2008 to perform western blot and immunoprecipitation in order to study the role of SUMO1 modification in stimuli-induced p100 phosphorylation and processing [ncbi , more ], to perform western blot in order to study the role of human metapneumovirus glycoprotein G in innate immune responses [ncbi , more ], to test the hypothesis that HGF suppresses renal inflammation by inhibiting proinflammatory cytokine expression [ncbi , more ], to perform western blot in order to study the effect of NFAT5 on hyperosmolar stressed human limbal epithelial cells [ncbi , more ]and to perform western blot in order to study the association between ING4 and the activity of NF-kappa B molecules bound with target gene promoters [ncbi , more ]. In 2007, Katrin Schweitzer et al. utilized Cell Signaling Technology p65 antibody to demonstrate that CSN controls NF-kappaB by deubiquitinylation of IkappaBalpha [ncbi , more ]. In 2006, Yoon Jae Song et al. utilized Cell Signaling Technology p65 antibody to study the role for IL-1 receptor-associated kinase 1 in latent membrane protein 1-induced p65/RelA serine 536 phosphorylation and NF-kappaB activation in human I1A 293 cells [ncbi , more ]. Cell Signaling Technology p65 antibody was applied in 2005 to study the effect of on E-selectin transcription [ncbi , more ], to detect the expression levels of phospho-p65/RelA Ser-536 in human C81 cells and mouse embryo fibroblasts [ncbi , more ]and to characterize a NIK and IKKbeta binding protein [ncbi , more ]. Cell Signaling Technology p65 antibody was utilized in 2004 to study the effect of multiple protein kinases and an unknown kinase and couples p65 to TATA-binding protein-associated factor II31-mediated interleukin-8 transcription on constitutive and interleukin-1-inducible phosphorylation of p65 NF- B at serine 536 [ncbi , more ], to detect phospho-p65 NF-kappaB in unstimulated and IL-1-treated HeLa cells incubated with a recombinant GST-p65- (354 551) fusion protein in the presence of [-32P]ATP [ncbi , more ], to detect p65 NF-kappaB and phospho-p65 NF-kappaB in human coronary artery endothelial cells (HCAEC) [ncbi , more ], to detect phospho-p65 in human peripheral T lymphocytes [ncbi , more ], to study the proapoptotic signaling pathways in interleukin-18-mediated human cardiac endothelial cell death [ncbi , more ]and to detect phospho-RelA/p65 in HUVECs pretreated with Y-27632 [ncbi , more ]. Hiroaki Sakurai et al. applied Cell Signaling Technology p65 antibody to study the regulatory mechanisms for the phosphorylation of p65 on Ser-536 under physiological conditions [ncbi , more ] in 2003 and Dirk Haller et al. used Cell Signaling Technology p65 antibody to study that transforming growth factor-beta 1 inhibits non-pathogenic Gram negative bacteria-induced NF-kappa B recruitment to the interleukin-6 gene promoter in intestinal epithelial cells through modulation of histone acetylation [ncbi , more ].
EMD MilliporeEMD Millipore p65 product
  • immunohistochemistryIn 2004, Susan E McNulty et al. applied EMD Millipore p65 antibody to study the expression pattern of NFkappaB family members in human melanocytic cells of normal skin, benign intradermal naevus, and metastatic melanoma tissues [ncbi , more ].
  • western blotIn 2009, Yun Song Lee et al. used EMD Millipore p65 antibody to perform western blot in order to show that JNK1 can suppresses the expression of LPS-induced MMP-9 [ncbi , more ]. Seol Hee Kim et al. employed EMD Millipore p65 antibody to perform western blot in order to show that thioredoxin is an IFN-gamma-induced factor and plays a role for cytokine production [ncbi , more , fig ] in 2008 and Branislava Janic et al. used EMD Millipore p65 antibody to demonstrate that endocytosed FePro nanoparticles did not significantly affect immunological properties of THP-1 cells [ncbi , more ]. In 2006, Ping Hu et al. applied EMD Millipore p65 antibody to study the mechanism by which ER stress activates NF-kappaB and its role in regulation of ER stress-induced cell death in human cells [ncbi , more ]. In 2005, Soo Jin Jeong et al. used EMD Millipore p65 antibody to detect p65/RelA levels in human C81 cells and mouse embryo fibroblasts [ncbi , more ].
Active MotifActive Motif p65 product
  • EMSAIn 2002, Pascal Knuefermann et al. employed Active Motif p65 antibody to study the role for myotrophin/V-1 in modulating the levels of activated NF B dimers in human HeLa cells [ncbi , more ].
Life Technologies CorporationLife Technologies Corporation p65 product
  • western blotIn 2005, Ling Chi Wang et al. applied Life Technologies Corporation p65 antibody to study tumor necrosis factor alpha-dependent drug resistance to purine and pyrimidine analogues in human colon tumor cells [ncbi , more ].
Rockland Immunochemicals, Inc.Rockland Immunochemicals, Inc. p65 product
  • ChIP assayIn 2003, Dirk Haller et al. used Rockland Immunochemicals, Inc. p65 antibody to study that transforming growth factor-beta 1 inhibits non-pathogenic Gram negative bacteria-induced NF-kappa B recruitment to the interleukin-6 gene promoter in intestinal epithelial cells through modulation of histone acetylation [ncbi , more ].
  • EMSAIn 2007, Natalie J Thornburg et al. utilized Rockland Immunochemicals, Inc. p65 antibody to study the role of NF-kappaB p50 and Bcl-3 in LMP1-mediated upregulation of EGFR in human cervical carcinoma cells [ncbi , more ]. In 2002, Sergio D Catz et al. applied Rockland Immunochemicals, Inc. p65 antibody to study the transcriptional activition of JFC1 by nuclear factor-kB and its regulation by tumour necrosis factor a in prostate carcinoma cells [ncbi , more ].
  • immunohistochemistryIn 2004, Susan E McNulty et al. applied Rockland Immunochemicals, Inc. p65 antibody to study the expression pattern of NFkappaB family members in human melanocytic cells of normal skin, benign intradermal naevus, and metastatic melanoma tissues [ncbi , more ].
  • immunoprecipitationDirk Haller et al. used Rockland Immunochemicals, Inc. p65 antibody to study that transforming growth factor-beta 1 inhibits non-pathogenic Gram negative bacteria-induced NF-kappa B recruitment to the interleukin-6 gene promoter in intestinal epithelial cells through modulation of histone acetylation [ncbi , more ] in 2003 and Ina Poser et al. used Rockland Immunochemicals, Inc. p65 antibody to study the effect of upregulation of HMG1 on melanoma inhibitory activity expression in malignant melanoma cells [ncbi , more ].
  • western blotIn 2007, Natalie J Thornburg et al. utilized Rockland Immunochemicals, Inc. p65 antibody to study the role of NF-kappaB p50 and Bcl-3 in LMP1-mediated upregulation of EGFR in human cervical carcinoma cells [ncbi , more ].
EpitomicsEpitomics p65 product
  • western blotIn 2009, Chad Myskiw et al. used Epitomics p65 antibody to carry out western blot analysis in order to investigate the mechanism for vaccinia virus double-stranded RNA binding protein E3 inhibiting the expression of cytokines [ncbi , more ].
AbcamAbcam p65 product
  • immunoprecipitationIn 2002, Pascal Knuefermann et al. employed Abcam p65 antibody to study the role for myotrophin/V-1 in modulating the levels of activated NF B dimers in human HeLa cells [ncbi , more ].
Enzo Life Sciences
  • western blotIn 2010, Junfang Wu et al. utilized Enzo Life Sciences p65 antibody to investigate the role of PKCdelta in the regulation of CHL1 expression in cultured astrocytes. [ncbi , more ]. In 2008, Patricia Méndez-Samperio et al. applied Enzo Life Sciences p65 antibody to study the role of TLR2 in CCL5 secretion in response to M. bovis BCG [ncbi , more ].
BioLegend
  • western blotIn 2008, Fei Huang et al. employed BioLegend p65 antibody to perform western blot in order to study the expression of IL-19 in airway epithelia induced by IL-17A and IL-4/IL-13 [ncbi , more ].
BD Biosciences
  • flow cytometryIn 2009, Marco Colombatti et al. applied BD Biosciences p65 antibody to study the role for prostate specific membrane antigen in regulating the expression of IL-6 and CCL5 in prostate tumour cells [ncbi , more ].
  • immunohistochemistryIn 2010, Nicholas Matluk et al. employed BD Biosciences p65 antibody to perform immunohistochemistry in order to determine the role of NRAGE in regulating NF-kappaB activity [ncbi , more ].
Roche Applied Science
  • western blotIn 2002, Chiara Fiorentini et al. applied Roche Applied Science p65 antibody to study the biological function of nerve growth factor in the regulation of dopamine D2 receptor expression [ncbi , more ].
Figure
Upstate Biotechnology anti-p65 was used to perform western blot in order to show that thioredoxin is an IFN-gamma-induced factor and plays a role for cytokine production.
p65 antibody western blot EMD Millipore
Analysis of activation and nuclear translocation of Stat1, NF-kappaB, and AP-1 induced by IFN-gamma in THP1 cells. Cells (5 x 106) were treated with media alone or IFN-gamma (10 ng/ml) under serum-free conditions for the indicated times. The cells were then harvested and used to prepare cytoplasmic or nuclear extracts. The extracts were then subjected to Western blot to determine the activation status of Stat1, c-Jun, c-fos or NF-kappaB, using antibodies to anti-phosphotyrosine Stat1, anti-Stat1, c-Jun, c-fos and NF-kappaB (p65). As internal controls, Cox-4 and H2A were used for cytosolic and nuclear marker proteins, respectively.
Seol Hee Kim et al. (2008). "Identification of human thioredoxin as a novel IFN-gamma-induced factor: mechanism of induction and its role in cytokine production".PMID 18983687
Articles Reviewed
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