This is a review about osteopontin, based on 28 published articles (read how Labome selects the articles), using osteopontin antibody in all methods. It is aimed to help Labome visitors find the most suited osteopontin antibody.
osteopontin synonym: BNSP; BSPI; ETA-1; OPN; SPP1/CALPHA1 fusion; early T-lymphocyte activation 1; nephropontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); secreted phosphoprotein 1 variant 6; urinary stone protein; uropontin

Santa Cruz Biotechnology
goat polyclonal (P-18)
  • WB; rat; 1:1000
Santa Cruz Biotechnology osteopontin antibody (Santa Cruz Biotechnology, sc-10593) was used in western blot on rat samples at 1:1000. Mol Cell Biochem (2015) ncbi
mouse monoclonal (AKm2A1)
  • WB; mouse
Santa Cruz Biotechnology osteopontin antibody (Santa Cruz Biotechnology, sc-21742) was used in western blot on mouse samples. J Mol Cell Cardiol (2015) ncbi
mouse monoclonal (AKm2A1)
  • IHC-P; mouse; 1:100; fig 7
Santa Cruz Biotechnology osteopontin antibody (Santa Cruz Biotechnology, sc-21742) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 7). Mol Med Rep (2015) ncbi
rabbit polyclonal (FL-314)
  • WB; mouse; 1:1000; fig 6
Santa Cruz Biotechnology osteopontin antibody (Santa Cruz Biotechnology, sc-20788) was used in western blot on mouse samples at 1:1000 (fig 6). Mol Med Rep (2015) ncbi
mouse monoclonal (AKm2A1)
  • WB; rat
Santa Cruz Biotechnology osteopontin antibody (Santa Cruz, sc-21742) was used in western blot on rat samples. Eur J Pharmacol (2014) ncbi
mouse monoclonal (AKm2A1)
  • IC; mouse
Santa Cruz Biotechnology osteopontin antibody (Santa Cruz, sc-21742) was used in immunocytochemistry on mouse samples. Proc Natl Acad Sci U S A (2014) ncbi
mouse monoclonal (AKm2A1)
  • WB; rat; 1:500
Santa Cruz Biotechnology osteopontin antibody (Santa Cruz, SC-21742) was used in western blot on rat samples at 1:500. Urology (2014) ncbi
goat polyclonal (K-20)
  • WB; human
Santa Cruz Biotechnology osteopontin antibody (Santa Cruz, sc-10591) was used in western blot on human samples. PLoS ONE (2013) ncbi
mouse monoclonal (AKm2A1)
  • IC; human; 2 ug/ml
Santa Cruz Biotechnology osteopontin antibody (Santa Cruz, sc-21742) was used in immunocytochemistry on human samples at 2 ug/ml. J Tissue Eng Regen Med (2015) ncbi
Abcam
rabbit polyclonal
  • IHC; human; 1:200
In order to evaluate the potential protein markers of bone metastases in breast cancer patients, Abcam osteopontin antibody (Abcam, ab 33046) was used in immunohistochemistry on human samples at 1:200. Pathol Oncol Res (2015) ncbi
rabbit polyclonal
  • IHC-P; mouse
Abcam osteopontin antibody (Abcam, ab8448) was used in immunohistochemistry - paraffin section on mouse samples. J Mol Cell Cardiol (2015) ncbi
rabbit polyclonal
  • IHC; mouse; 1:200
In order to investigate the role of WDC1 during infection and wound healing using wdc1-null mice, Abcam osteopontin antibody (Abcam, ab8448) was used in immunohistochemistry on mouse samples at 1:200. Am J Pathol (2014) ncbi
rabbit polyclonal
  • WB; mouse
Abcam osteopontin antibody (Abcam, ab8448) was used in western blot on mouse samples. J Cell Mol Med (2014) ncbi
rabbit polyclonal
  • IHC-P; rat; 1:300
Abcam osteopontin antibody (Abcam, ab8448) was used in immunohistochemistry - paraffin section on rat samples at 1:300. Urology (2014) ncbi
rabbit polyclonal
  • IHC-F; human
Abcam osteopontin antibody (Abcam, ab33046) was used in immunohistochemistry - frozen section on human samples. Stem Cells (2014) ncbi
rabbit polyclonal
  • IC; human
Abcam osteopontin antibody (Abcam, ab8448) was used in immunocytochemistry on human samples. J Biomed Mater Res A (2014) ncbi
rabbit polyclonal
  • IHC-P; mouse; 1:100
  • WB; mouse; 1:1000
Abcam osteopontin antibody (Abcam, ab8448) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 and in western blot on mouse samples at 1:1000. J Biomed Mater Res B Appl Biomater (2013) ncbi
mouse monoclonal (53)
  • IHC-P; human; 1:400
Abcam osteopontin antibody (Abcam, ab69498) was used in immunohistochemistry - paraffin section on human samples at 1:400. Oncology (2013) ncbi
rabbit polyclonal
  • IHC-P; mouse; 1:400
Abcam osteopontin antibody (Abcam, ab8448) was used in immunohistochemistry - paraffin section on mouse samples at 1:400. J Cell Physiol (2013) ncbi
not indicated
  • IHC; human
Abcam rabbit polyclonal anti-SPP1 Ig antibody was used in immunohistochemistry to investigate the role of osteopontin and immune related factors in the regulation of the palatal development.Exp Mol Med (2009) ncbi
Novus Biologicals
mouse monoclonal (1B20)
  • IHC-P; rabbit; 1:100
  • IC; rabbit; 1:200
Novus Biologicals osteopontin antibody (Novus Biologicals, NB110-89062) was used in immunohistochemistry - paraffin section on rabbit samples at 1:100 and in immunocytochemistry on rabbit samples at 1:200. Tissue Eng Part A (2014) ncbi
Epitomics
rabbit monoclonal
  • IHC-P; human; 1:50
In order to screen a panel of 33 proteins to establish a set of biomarkers that predict the agressiveness of prostate cancer, Epitomics osteopontin antibody (Epitomics, 2671-1) was used in immunohistochemistry - paraffin section on human samples at 1:50. BMC Cancer (2014) ncbi
Enzo Life Sciences
mouse monoclonal (53)
  • WB; human; 1:2,000
Enzo Life Sciences osteopontin antibody (Enzo Life Sciences, ADI-905-629-100) was used in western blot on human samples at 1:2,000. Cancer Biol Ther (2011) ncbi
not indicated
  • ELISA; human
Assay Designs OPN ELISA kit was used to measure OPN in cell culture supernatants and tissue homogenates in order to study spontaneous metastatic performance of orthotopic human breast cancer xenograftsAm J Pathol (2007) ncbi
R and D Systems
  • ELISA; human
R and D Systems osteopontin antibody (R&D, DOST00) was used in ELISA on human samples. PLoS ONE (2012) ncbi
not indicatedR&D Systems osteopontin ELISA kit was used to measure the level of osteopontin in the culture supernatant in order to investigate the mechanism for BCR signaling pathway in the regulation of osteopontin production and secretion when B cells is exposed to IL-4.Mol Immunol (2009) ncbi
EMD Millipore
not indicated
  • IHC; human
Calbiochem anti-osteopontin antibody (1:100) was used to carry out immunofluorescence assays in order to study whether c-Src kinase contributes the progression from atypical lobular hyperplasia, and lobular carcinoma in situ to invasive lobular carcinoma.Breast Cancer Res (2009) ncbi
not indicated
  • WB; human
Chemicon International monoclonal anti-human osteopontin antibody (1:2000) was used in western blot to study spontaneous metastatic performance of orthotopic human breast cancer xenograftsAm J Pathol (2007) ncbi
Invitrogen
not indicatedApplied Biosystems PCR primers for osteopontin (SPP1) was used in real-time quantitative RT-PCR in order to study the effect of the large isoform of MRJ (DNAJB6) on malignant activity of breast cancer.Breast Cancer Res (2008) ncbi
not indicatedApplied Biosystems probes for osteopontin (SPP1) was used in real-time quantitative RT-PCR in order to study the effect of the large isoform of MRJ (DNAJB6) on malignant activity of breast cancer.Breast Cancer Res (2008) ncbi
Developmental Studies Hybridoma Bank
not indicated
  • IHC; human
Developmental Studies Hybridoma Bank osteopontin antibody (1:500) was used in immunohistochemistry to study the role of renin-angiotensin system (RAS) dysregulation in inducing Kim-1.Am J Physiol Renal Physiol (2007) ncbi
mouse monoclonal (MPIIIB10(1))
  • IC; human; 1:5
Developmental Studies Hybridoma Bank osteopontin antibody (DSHB, MPIIIB10) was used in immunocytochemistry on human samples at 1:5. Anticancer Res (2004) ncbi
Sigma-Aldrich
not indicated
  • WB; human
Sigma anti-osteopontin antibody was used in western blot to study the effect of the large isoform of MRJ (DNAJB6) on malignant activity of breast cancer.Breast Cancer Res (2008) ncbi
GE Healthcare Life Biosciences
not indicated
  • ELISA; human
Open Biosystems human cDNA for OPN was used to perform protein expression in order to investigate the involvement of Fam20C in the phosphorylation of extracellular proteins.Science (2012) ncbi
Articles Reviewed
  1. Piotr Winczura et al. (2015). "Immunohistochemical Predictors of Bone Metastases in Breast Cancer Patients".PMID 26062800
  2. Tingjiao Liu et al. (2015). "Vascular smooth muscle cell differentiation to an osteogenic phenotype involves matrix metalloproteinase-2 modulation by homocysteine".PMID 25987498
  3. Kyo Won Seo et al. (2015). "Mechanical stretch enhances the expression and activity of osteopontin and MMP-2 via the Akt1/AP-1 pathways in VSMC".PMID 25986148
  4. Koji Harada et al. (2015). "Osteogenic cell fractions isolated from mouse tongue muscle".PMID 25684092
  5. Steven J Ressler et al. (2014). "WFDC1 is a key modulator of inflammatory and wound repair responses".PMID 25219356
  6. Zhaoliang Su et al. (2014). "Up-regulated HMGB1 in EAM directly led to collagen deposition by a PKCĪ²/Erk1/2-dependent pathway: cardiac fibroblast/myofibroblast might be another source of HMGB1".PMID 24912759
  7. Joseph Fomusi Ndisang et al. (2014). "Heme oxygenase suppresses markers of heart failure and ameliorates cardiomyopathy in L-NAME-induced hypertension".PMID 24726875
  8. Anthony E Rizzardi et al. (2014). "Evaluation of protein biomarkers of prostate cancer aggressiveness".PMID 24708576
  9. Makoto Inoue et al. (2014). "T cells down-regulate macrophage TNF production by IRAK1-mediated IL-10 expression and control innate hyperinflammation".PMID 24706909
  10. Thuy Ba Linh Nguyen et al. (2014). "A combination of biphasic calcium phosphate scaffold with hyaluronic acid-gelatin hydrogel as a new tool for bone regeneration".PMID 24517159
  11. Zhaohui Jia et al. (2014). "Does crystal deposition in genetic hypercalciuric rat kidney tissue share similarities with bone formation?".PMID 24468523
  12. Francesca Paino et al. (2014). "Histone deacetylase inhibition with valproic acid downregulates osteocalcin gene expression in human dental pulp stem cells and osteoblasts: evidence for HDAC2 involvement".PMID 24105979
  13. Jekaterina Kazantseva et al. (2013). "Alternative splicing targeting the hTAF4-TAFH domain of TAF4 represses proliferation and accelerates chondrogenic differentiation of human mesenchymal stem cells".PMID 24098348
  14. A R P Silva et al. (2014). "Synergistic effect between bioactive glass foam and a perfusion bioreactor on osteogenic differentiation of human adipose stem cells".PMID 23625853
  15. Kyuseok Choi et al. (2013). "Low-level laser therapy promotes the osteogenic potential of adipose-derived mesenchymal stem cells seeded on an acellular dermal matrix".PMID 23529895
  16. Luciano de Souza Viana et al. (2013). "Relationship between the expression of the extracellular matrix genes SPARC, SPP1, FN1, ITGA5 and ITGAV and clinicopathological parameters of tumor progression and colorectal cancer dissemination".PMID 23128103
  17. Steve Goodison et al. (2012). "A multi-analyte assay for the non-invasive detection of bladder cancer".PMID 23094052
  18. Sidan Li et al. (2013). "A pivotal role of bone remodeling in granulocyte colony stimulating factor induced hematopoietic stem/progenitor cells mobilization".PMID 23042582
  19. Emilia Kaivosoja et al. (2015). "The effect of pulsed electromagnetic fields and dehydroepiandrosterone on viability and osteo-induction of human mesenchymal stem cells".PMID 23038647
  20. Vincent S Tagliabracci et al. (2012). "Secreted kinase phosphorylates extracellular proteins that regulate biomineralization".PMID 22582013
  21. Jennifer C Mutrie et al. (2011). "Osteopontin increases breast cancer cell sensitivity to specific signaling pathway inhibitors in preclinical models".PMID 21795853
  22. Donghui Zou et al. (2009). "Increased levels of active c-Src distinguish invasive from in situ lobular lesions".PMID 19583841
  23. Linda P Jakobsen et al. (2009). "Expression analyses of human cleft palate tissue suggest a role for osteopontin and immune related factors in palatal development".PMID 19287188
  24. Benchang Guo et al. (2009). "B cell receptor crosstalk: B cells express osteopontin through the combined action of the alternate and classical BCR signaling pathways".PMID 18952291
  25. Aparna Mitra et al. (2008). "Large isoform of MRJ (DNAJB6) reduces malignant activity of breast cancer".PMID 18328103
  26. Mika Suzuki et al. (2007). "Osteopontin gene expression determines spontaneous metastatic performance of orthotopic human breast cancer xenografts".PMID 17620367
  27. Martin H de Borst et al. (2007). "Induction of kidney injury molecule-1 in homozygous Ren2 rats is attenuated by blockade of the renin-angiotensin system or p38 MAP kinase".PMID 16896183
  28. Christoph Pautke et al. (2004). "Characterization of osteosarcoma cell lines MG-63, Saos-2 and U-2 OS in comparison to human osteoblasts".PMID 15736406