This is a review about leptin, based on 6 published articles (read how Labome selects the articles), using leptin antibody in all methods. It is aimed to help Labome visitors find the most suited leptin antibody.
leptin synonym: LEPD; OBS; leptin (murine obesity homolog); leptin (obesity homolog, mouse); obese protein; obese, mouse, homolog of; obesity factor

Santa Cruz Biotechnology
rabbit polyclonal (H-146)
  • WB; human; 1:100-400
  • WB; rat; 1:100-400
In order to investigate apelin signaling in brown adipocyte tissue, Santa Cruz Biotechnology leptin antibody (Santa Cruz Biotechnology, sc-9014) was used in western blot on human samples at 1:100-400 and on rat samples at 1:100-400. J Biol Chem (2015) ncbi
rabbit polyclonal (H-146)
  • WB; human
Santa Cruz Biotechnology leptin antibody (Santa Cruz, SC-9014) was used in western blot on human samples. J Vis Exp (2014) ncbi
rabbit polyclonal (A-20)
  • WB; rat
  • IHC; rat
Santa Cruz Biotechnology leptin antibody (Santa Cruz Biotech., sc-842) was used in western blot on rat samples and in immunohistochemistry on rat samples. Biotech Histochem (2014) ncbi
rabbit polyclonal (H-146)
  • WB; human
Santa Cruz Biotechnology anti-Ob antibody was used to carry out western blot analysis in order to identify the binding sites of PPAR Gramma and RXR in a genome wide manner during adipogenesis.PLoS ONE (2009) ncbi
Invitrogen
rabbit polyclonal
  • IHC-P; sheep; 1:500
In order to measure FGF2, Ang1, VEGF, ObRb, and leptin expression in the corpus luteum of goats, Invitrogen leptin antibody (Thermo Scientific, PA1-052) was used in immunohistochemistry - paraffin section on sheep samples at 1:500. Anim Reprod Sci (2014) ncbi
R and D Systems
not indicated
  • ELISA; human
R&D Systems ELISA kit for leptin was used in ELISA to determine the quantity of adiponectin in the lysates of human HEK293 cells.J Biol Chem (2006) ncbi
Articles Reviewed
  1. Aung Than et al. (2015). "Apelin Enhances Brown Adipogenesis and Browning of White Adipocytes".PMID 25931124
  2. Jessica R Wiles et al. (2014). "The effect of leptin on luteal angiogenic factors during the luteal phase of the estrous cycle in goats".PMID 24962614
  3. Melissa V Fernandez et al. (2014). "Activation and measurement of NLRP3 inflammasome activity using IL-1β in human monocyte-derived dendritic cells".PMID 24894187
  4. M Ates et al. (2014). "Anxiety- and depression-like behavior are correlated with leptin and leptin receptor expression in prefrontal cortex of streptozotocin-induced diabetic rats".PMID 24007473
  5. Mohamed Sabry Hamza et al. (2009). "De-novo identification of PPARgamma/RXR binding sites and direct targets during adipogenesis".PMID 19300518
  6. Linglin Xie et al. (2006). "Intracellular trafficking and secretion of adiponectin is dependent on GGA-coated vesicles".PMID 16407204