This is a review about huntingtin, based on 49 published articles (read how Labome selects the articles), using huntingtin antibody in all methods. It is aimed to help Labome visitors find the most suited huntingtin antibody.
huntingtin synonym: IT15; huntington disease protein

EMD Millipore
mouse monoclonal (1HU-4C8)
  • WB; human; 1:1000
  • WB; mouse; 1:1000
  • IP; human
  • IP; mouse
In order to assess transgenic mice expressing different forms of huntingtin protein for disease, EMD Millipore huntingtin antibody (Millipore, MAB2166) was used in western blot on human samples at 1:1000 and on mouse samples at 1:1000 and in immunoprecipitation on human samples and on mouse samples. J Biol Chem (2015) ncbi
mouse monoclonal (mEM48)
  • IHC-P; mouse; 1:50
  • WB; mouse; 1:500
In order to assess transgenic mice expressing different forms of huntingtin protein for disease, EMD Millipore huntingtin antibody (Millipore, MAB5374) was used in immunohistochemistry - paraffin section on mouse samples at 1:50 and in western blot on mouse samples at 1:500. J Biol Chem (2015) ncbi
mouse monoclonal
  • WB; mouse; 1:500
In order to assess transgenic mice expressing different forms of huntingtin protein for disease, EMD Millipore huntingtin antibody (Millipore, MAB5490) was used in western blot on mouse samples at 1:500. J Biol Chem (2015) ncbi
mouse monoclonal (mEM48)
  • IHC-F; mouse; fig 6e
In order to demonstrate that G-protein-coupled receptors modulate the levels of Atg14L through ZBTB16, EMD Millipore huntingtin antibody (Millipore, MAB5374) was used in immunohistochemistry - frozen section on mouse samples (fig 6e). elife (2015) ncbi
mouse monoclonal (1HU-4C8)
  • WB; mouse
EMD Millipore huntingtin antibody (EMD Milliporeon, MAB2166) was used in western blot on mouse samples. Dev Biol (2015) ncbi
mouse monoclonal (1HU-4C8)
  • WB; human
EMD Millipore huntingtin antibody (Millipore, 1HU-4C8) was used in western blot on human samples. Dis Model Mech (2014) ncbi
mouse monoclonal (mEM48)
  • IHC-Free; mouse; 1:100
EMD Millipore huntingtin antibody (Millipore, MAB5374) was used in immunohistochemistry - free floating section on mouse samples at 1:100. Neurobiol Dis (2015) ncbi
mouse monoclonal (mEM48)
  • IHC-P; mouse; 1:500
In order to investigate the relationship between mutant huntingtin and Tau in murine models of Huntington's disease, EMD Millipore huntingtin antibody (Millipore, MAB5374) was used in immunohistochemistry - paraffin section on mouse samples at 1:500. Hum Mol Genet (2015) ncbi
mouse monoclonal (mEM48)
  • IHC-Free; human
EMD Millipore huntingtin antibody (Millipore, MAB5374) was used in immunohistochemistry - free floating section on human samples. PLoS ONE (2014) ncbi
mouse monoclonal (mEM48)
  • WB; rat; 1:1000
  • IC; rat; 1:1000
EMD Millipore huntingtin antibody (Millipore, MAB5374) was used in western blot on rat samples at 1:1000 and in immunocytochemistry on rat samples at 1:1000. J Neurochem (2014) ncbi
rabbit polyclonal
  • WB; rat; 1:500
EMD Millipore huntingtin antibody (Chemicon, AB9562) was used in western blot on rat samples at 1:500. J Neurochem (2014) ncbi
mouse monoclonal (mEM48)
  • WB; mouse; 1:1000
EMD Millipore huntingtin antibody (Millipore, MAB5374) was used in western blot on mouse samples at 1:1000. J Huntingtons Dis (2013) ncbi
mouse monoclonal (1HU-4C8)
  • WB; mouse; 1:2000
EMD Millipore huntingtin antibody (Chemicon, MAB2166) was used in western blot on mouse samples at 1:2000. J Huntingtons Dis (2012) ncbi
mouse monoclonal (1HU-4C8)
  • IHC-P; human; 1:150
EMD Millipore huntingtin antibody (Millipore, MAB2166) was used in immunohistochemistry - paraffin section on human samples at 1:150. PLoS ONE (2014) ncbi
mouse monoclonal (mEM48)
  • IHC; mouse; 1:100
In order to investigate the role of tau missplicing in the pathogenesis of Huntington disease, EMD Millipore huntingtin antibody (Millipore, MAB5374) was used in immunohistochemistry on mouse samples at 1:100. Nat Med (2014) ncbi
mouse monoclonal (1HU-4C8)
  • WB; human; 1:10000
EMD Millipore huntingtin antibody (Millipore, MAB2166) was used in western blot on human samples at 1:10000. PLoS ONE (2014) ncbi
mouse monoclonal (1HU-4C8)
  • IHC-P; human; 1:500
In order to investigate the association of eosinophilic neuronal cytoplasmic inclusions with stress granules and autophagy in two clinical cases, EMD Millipore huntingtin antibody (Millipore, 1HU-4C8) was used in immunohistochemistry - paraffin section on human samples at 1:500. Neuropathology (2014) ncbi
mouse monoclonal (1HU-4C8)
  • WB; human; 1:1000
EMD Millipore huntingtin antibody (EMD Millipore, MAB2166) was used in western blot on human samples at 1:1000. Ann Neurol (2014) ncbi
mouse monoclonal (mEM48)
  • IHC; human; 1:2000
EMD Millipore huntingtin antibody (EMD Millipore, MAB5374) was used in immunohistochemistry on human samples at 1:2000. Ann Neurol (2014) ncbi
mouse monoclonal (1HU-4C8)
  • WB; human; 1:1000
EMD Millipore huntingtin antibody (Millipore, MAB2166) was used in western blot on human samples at 1:1000. PLoS Curr (2014) ncbi
mouse monoclonal (1HU-4C8)
  • WB; African green monkey; 1:1000
EMD Millipore huntingtin antibody (Millipore, MAB2166) was used in western blot on African green monkey samples at 1:1000. PLoS ONE (2014) ncbi
mouse monoclonal (1HU-4C8)
  • IHC-P; human; 1:200
EMD Millipore huntingtin antibody (Chemicon, MAB2166) was used in immunohistochemistry - paraffin section on human samples at 1:200. Neuropathol Appl Neurobiol (2014) ncbi
mouse monoclonal (1HU-4C8)
  • WB; human
  • IP; human
EMD Millipore huntingtin antibody (Chemicon, 2166) was used in western blot on human samples and in immunoprecipitation on human samples. J Biol Chem (2014) ncbi
mouse monoclonal (1HU-4C8)
  • WB; human
EMD Millipore huntingtin antibody (Chemicon, mAB 2166) was used in western blot on human samples. PLoS ONE (2014) ncbi
mouse monoclonal (1HU-4C8)
  • WB; mouse
In order to investigate the role of huntingtin in the the regulation of autophagosome dynamics, EMD Millipore huntingtin antibody (Millipore, MAB2166) was used in western blot on mouse samples. J Neurosci (2014) ncbi
mouse monoclonal (1HU-4C8)
  • WB; human; 1:1,000
EMD Millipore huntingtin antibody (Millipore, MAB2166) was used in western blot on human samples at 1:1,000. Nucleic Acid Ther (2014) ncbi
mouse monoclonal (1HU-4C8)
  • WB; mouse; 1:2000
EMD Millipore huntingtin antibody (Millipore, MAB2166) was used in western blot on mouse samples at 1:2000. PLoS ONE (2013) ncbi
mouse monoclonal (mEM48)
  • WB; rat; 1:1000
EMD Millipore huntingtin antibody (Millipore, MAB5374) was used in western blot on rat samples at 1:1000. J Biol Chem (2014) ncbi
mouse monoclonal (1HU-4C8)
  • WB; human
EMD Millipore huntingtin antibody (Millipore, MAB2166) was used in western blot on human samples. Biochemistry (2013) ncbi
mouse monoclonal (mEM48)
  • WB; mouse; 1:100
EMD Millipore huntingtin antibody (Chemicon, MAB5374) was used in western blot on mouse samples at 1:100. PLoS ONE (2013) ncbi
mouse monoclonal (1HU-4C8)
  • IP; human
EMD Millipore huntingtin antibody (Millipore, MAB2166) was used in immunoprecipitation on human samples. Cell Rep (2013) ncbi
mouse monoclonal (1HU-4C8)
  • WB; human
EMD Millipore huntingtin antibody (Chemicon Millipore, MAB2166) was used in western blot on human samples. Nucleic Acids Res (2013) ncbi
mouse monoclonal
  • IP; human
  • WB; human
EMD Millipore huntingtin antibody (Millipore, MAB5490) was used in immunoprecipitation on human samples and in western blot on human samples. Cell Rep (2013) ncbi
mouse monoclonal (1HU-4C8)
  • WB; mouse
EMD Millipore huntingtin antibody (Millipore, MAB2166) was used in western blot on mouse samples. J Huntingtons Dis (2012) ncbi
mouse monoclonal (mEM48)
  • IHC; mouse
EMD Millipore huntingtin antibody (Millipore, MAB5374) was used in immunohistochemistry on mouse samples. J Neurosci (2013) ncbi
mouse monoclonal (1HU-4C8)
  • IHC-P; mouse; 1:50
EMD Millipore huntingtin antibody (Chemicon / Millipore, MAB2166) was used in immunohistochemistry - paraffin section on mouse samples at 1:50. J Comp Neurol (2013) ncbi
mouse monoclonal (1HU-4C8)
  • WB; mouse; 1:250
EMD Millipore huntingtin antibody (Millipore, MAB2166) was used in western blot on mouse samples at 1:250. Mitochondrion (2013) ncbi
mouse monoclonal (1HU-4C8)
  • WB; rat
  • IHC; rat
EMD Millipore huntingtin antibody (Millipore, MAB2166) was used in western blot on rat samples and in immunohistochemistry on rat samples. Antioxid Redox Signal (2013) ncbi
mouse monoclonal (mEM48)
  • IC; human
EMD Millipore huntingtin antibody (Millipore, MAB5374) was used in immunocytochemistry on human samples. FASEB J (2013) ncbi
mouse monoclonal (mEM48)
  • IHC-F; mouse; 1:250
EMD Millipore huntingtin antibody (Millipore, MAB5374) was used in immunohistochemistry - frozen section on mouse samples at 1:250. PLoS ONE (2012) ncbi
mouse monoclonal (mEM48)
  • IHC-Free; mouse; 1:300
EMD Millipore huntingtin antibody (Millipore, MAB5374) was used in immunohistochemistry - free floating section on mouse samples at 1:300. Eur J Neurosci (2013) ncbi
mouse monoclonal (mEM48)
  • WB; mouse
EMD Millipore huntingtin antibody (Chemicon, MAB5374) was used in western blot on mouse samples. Eur J Pharmacol (2013) ncbi
mouse monoclonal (1HU-4C8)
  • WB; rat; 1:2,500
EMD Millipore huntingtin antibody (Millipore, MAB2166) was used in western blot on rat samples at 1:2,500. Mol Pharm (2013) ncbi
mouse monoclonal (mEM48)
  • IHC-Free; rat; 1:300
EMD Millipore huntingtin antibody (Millipore, MAB5374) was used in immunohistochemistry - free floating section on rat samples at 1:300. J Neurosci (2012) ncbi
mouse monoclonal (1HU-4C8)
  • WB; human; 1:2000
  • WB; rat; 1:2000
EMD Millipore huntingtin antibody (Millipore, MAB2166) was used in western blot on human samples at 1:2000 and on rat samples at 1:2000. J Neurosci (2012) ncbi
mouse monoclonal (1HU-4C8)
  • IP; human
  • IC; human
  • WB; human
EMD Millipore huntingtin antibody (Chemicon, MAB2166) was used in immunoprecipitation on human samples, in immunocytochemistry on human samples, and in western blot on human samples. PLoS ONE (2012) ncbi
mouse monoclonal (1HU-4C8)
  • WB; human
EMD Millipore huntingtin antibody (Chemicon, MAB2166) was used in western blot on human samples. Nucleic Acids Res (2012) ncbi
not indicated
  • WB; human
  • IC; human
Chemicon-Milliporeanti-huntingtin antibody was used for immunoblotting in order to illustrate the full-length huntingtin's helical organization and role as a facilitator of the multi-subunit PRC2 complex.Hum Mol Genet (2010) ncbi
not indicated
  • IC; human
Chemicon Htt antibody was used in immunocytochemistry in oeder to demonstrate that huntington's disease protein contributes to RNA-mediated gene silencingProc Natl Acad Sci U S A (2008) ncbi
not indicated
  • ChIP; human
Chemicon International mouse monoclonal anti-Htt antibody MAB2166 and rat monoclonal anti-Htt antibody MAB2174 were used in ChIP assay to study the effect of Huntingtin on dynein/dynactin-mediated vesicle transport in human HeLa-M cells.Proc Natl Acad Sci U S A (2007) ncbi
Santa Cruz Biotechnology
goat polyclonal (N-18)
  • IHC; mouse; 1:100
Santa Cruz Biotechnology huntingtin antibody (Santa Cruz, SC-8767) was used in immunohistochemistry on mouse samples at 1:100. Exp Neurol (2013) ncbi
not indicated
  • IHC; human
Santa Cruz huntingtin antibody (1:100) was used immunohistochemistry in order to investigate the toxic effects of glial cells in the regulation of several neurodegenerative disordersPLoS ONE (2009) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (MW1)
  • WB; human; 1:500
Developmental Studies Hybridoma Bank huntingtin antibody (Developmental Studies Hybridoma Bank, MW1) was used in western blot on human samples at 1:500. PLoS ONE (2014) ncbi
mouse monoclonal (MW7)
  • IHC; human; 1:100
Developmental Studies Hybridoma Bank huntingtin antibody (Developmental Studies Hybridoma Bank, MW7) was used in immunohistochemistry on human samples at 1:100. Ann Neurol (2014) ncbi
Sigma-Aldrich
rabbit polyclonal
  • WB; mouse; 1:200
In order to assess transgenic mice expressing different forms of huntingtin protein for disease, Sigma-Aldrich huntingtin antibody (Sigma, H7540) was used in western blot on mouse samples at 1:200. J Biol Chem (2015) ncbi
mouse monoclonal (3B5H10)
  • IP; mouse
Sigma-Aldrich huntingtin antibody (Sigma, 3B5H10) was used in immunoprecipitation on mouse samples. Proc Natl Acad Sci U S A (2013) ncbi
Euromedex
not indicated
  • IC; human
anti-Huntingtin antibody was used for immunofluorescence assay, in order to study the role of Huntingtin in apical polarity during the morphogenesis of the mouse mammary epitheliumPLoS Biol (2015) ncbi
Cell Signaling Technology
not indicated
  • IC; human
anti-Huntingtin antibody was used for immunofluorescence assay, in order to study the role of Huntingtin in apical polarity during the morphogenesis of the mouse mammary epitheliumPLoS Biol (2015) ncbi
Articles Reviewed
  1. Robert O'Brien et al. (2015). "Integration-independent Transgenic Huntington Disease Fragment Mouse Models Reveal Distinct Phenotypes and Life Span in Vivo".PMID 26025364
  2. Salah Elias et al. (2015). "Huntingtin Is Required for Epithelial Polarity through RAB11A-Mediated Apical Trafficking of PAR3-aPKC".PMID 25942483
  3. Tao Zhang et al. (2015). "G-protein-coupled receptors regulate autophagy by ZBTB16-mediated ubiquitination and proteasomal degradation of Atg14L".PMID 25821988
  4. Shaun S Sanders et al. (2015). "Huntingtin interacting proteins 14 and 14-like are required for chorioallantoic fusion during early placental development".PMID 25478910
  5. Hemma Brandstaetter et al. (2014). "Huntingtin is required for ER-to-Golgi transport and for secretory vesicle fusion at the plasma membrane".PMID 25368120
  6. J G Doria et al. (2015). "The mGluR5 positive allosteric modulator, CDPPB, ameliorates pathology and phenotypic signs of a mouse model of Huntington's disease".PMID 25160573
  7. David Blum et al. (2015). "Mutant huntingtin alters Tau phosphorylation and subcellular distribution".PMID 25143394
  8. Silvia Corrochano et al. (2014). "Reducing Igf-1r levels leads to paradoxical and sexually dimorphic effects in HD mice".PMID 25140802
  9. Kirstie H Stansfield et al. (2014). "BDNF and Huntingtin protein modifications by manganese: implications for striatal medium spiny neuron pathology in manganese neurotoxicity".PMID 25099302
  10. Hongyu Zhang et al. (2013). "NGF rescues hippocampal cholinergic neuronal markers, restores neurogenesis, and improves the spatial working memory in a mouse model of Huntington's Disease".PMID 25063430
  11. Rona K Graham et al. (2012). "Caspase-6-Resistant Mutant Huntingtin Does not Rescue the Toxic Effects of Caspase-Cleavable Mutant Huntingtin in vivo".PMID 25063333
  12. Pasquale Simeone et al. (2014). "A unique four-hub protein cluster associates to glioblastoma progression".PMID 25050814
  13. Marta Fernández-Nogales et al. (2014). "Huntington's disease is a four-repeat tauopathy with tau nuclear rods".PMID 25038828
  14. Douglas Macdonald et al. (2014). "Quantification assays for total and polyglutamine-expanded huntingtin proteins".PMID 24816435
  15. Fumiaki Mori et al. (2014). "Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases".PMID 24812700
  16. Francesca Cicchetti et al. (2014). "Mutant huntingtin is present in neuronal grafts in Huntington disease patients".PMID 24798518
  17. Mahru C An et al. (2014). "Polyglutamine Disease Modeling: Epitope Based Screen for Homologous Recombination using CRISPR/Cas9 System".PMID 24761311
  18. Shaun S Sanders et al. (2014). "Identification of binding sites in Huntingtin for the Huntingtin Interacting Proteins HIP14 and HIP14L".PMID 24651384
  19. Davide Schiffer et al. (2014). "Repressor element-1 silencing transcription factor (REST) is present in human control and Huntington's disease neurones".PMID 24634989
  20. Sébastien Marion et al. (2014). "Dopamine D2 receptor relies upon PPM/PP2C protein phosphatases to dephosphorylate huntingtin protein".PMID 24619418
  21. Maria Ángeles Fernández-Estevez et al. (2014). "Trehalose reverses cell malfunction in fibroblasts from normal and Huntington's disease patients caused by proteosome inhibition".PMID 24587280
  22. Yvette C Wong et al. (2014). "The regulation of autophagosome dynamics by huntingtin and HAP1 is disrupted by expression of mutant huntingtin, leading to defective cargo degradation".PMID 24453320
  23. Melvin M Evers et al. (2014). "Preventing formation of toxic N-terminal huntingtin fragments through antisense oligonucleotide-mediated protein modification".PMID 24380395
  24. Barbara Baldo et al. (2013). "Maintenance of basal levels of autophagy in Huntington's disease mouse models displaying metabolic dysfunction".PMID 24376631
  25. Aaron Y Lai et al. (2014). "scyllo-Inositol promotes robust mutant Huntingtin protein degradation".PMID 24352657
  26. Yuichiro Aiba et al. (2013). "Allele-selective inhibition of expression of huntingtin and ataxin-3 by RNA duplexes containing unlocked nucleic acid substitutions".PMID 24266403
  27. Juan Perucho et al. (2013). "Striatal infusion of glial conditioned medium diminishes huntingtin pathology in r6/1 mice".PMID 24069174
  28. Juan I Sbodio et al. (2013). "Golgi protein ACBD3 mediates neurotoxicity associated with Huntington's disease".PMID 24012756
  29. Jing Liu et al. (2013). "RNA duplexes with abasic substitutions are potent and allele-selective inhibitors of huntingtin and ataxin-3 expression".PMID 23887934
  30. Jacqueline Gire O'Rourke et al. (2013). "SUMO-2 and PIAS1 modulate insoluble mutant huntingtin protein accumulation".PMID 23871671
  31. Melissa K Strong et al. (2012). "Age-Dependent Resistance to Excitotoxicity in Htt CAG140 Mice and the Effect of Strain Background".PMID 23833693
  32. Luis M Valor et al. (2013). "Genomic landscape of transcriptional and epigenetic dysregulation in early onset polyglutamine disease".PMID 23785159
  33. Pavel Milman et al. (2013). "Novel variant of neuronal intranuclear rodlet immunoreactive for 40 kDa huntingtin associated protein and ubiquitin in the mouse brain".PMID 23749422
  34. Ana C Silva et al. (2013). "Mitochondrial respiratory chain complex activity and bioenergetic alterations in human platelets derived from pre-symptomatic and symptomatic Huntington's disease carriers".PMID 23707479
  35. Florian Haun et al. (2013). "S-nitrosylation of dynamin-related protein 1 mediates mutant huntingtin-induced mitochondrial fragmentation and neuronal injury in Huntington's disease".PMID 23641925
  36. Kirupa Sathasivam et al. (2013). "Aberrant splicing of HTT generates the pathogenic exon 1 protein in Huntington disease".PMID 23341618
  37. Boxun Lu et al. (2013). "A novel human embryonic stem cell-derived Huntington's disease neuronal model exhibits mutant huntingtin (mHTT) aggregates and soluble mHTT-dependent neurodegeneration".PMID 23325320
  38. H Akiko Popiel et al. (2012). "Hsp40 gene therapy exerts therapeutic effects on polyglutamine disease mice via a non-cell autonomous mechanism".PMID 23226463
  39. S E Dougherty et al. (2013). "Purkinje cell dysfunction and loss in a knock-in mouse model of Huntington disease".PMID 23195593
  40. Eric A Horne et al. (2013). "Downregulation of cannabinoid receptor 1 from neuropeptide Y interneurons in the basal ganglia of patients with Huntington's disease and mouse models".PMID 23167744
  41. Awahan Rahman et al. (2013). "Late onset vascular dysfunction in the R6/1 model of Huntington's disease".PMID 23117088
  42. Bruno M D C Godinho et al. (2013). "Self-assembling modified β-cyclodextrin nanoparticles as neuronal siRNA delivery vectors: focus on Huntington's disease".PMID 23116281
  43. Libo Yu-Taeger et al. (2012). "A novel BACHD transgenic rat exhibits characteristic neuropathological features of Huntington disease".PMID 23115180
  44. Lin Qi et al. (2012). "The role of chaperone-mediated autophagy in huntingtin degradation".PMID 23071649
  45. Jiaxin Hu et al. (2012). "Mechanism of allele-selective inhibition of huntingtin expression by duplex RNAs that target CAG repeats: function through the RNAi pathway".PMID 23042244
  46. Ihn Sik Seong et al. (2010). "Huntingtin facilitates polycomb repressive complex 2".PMID 19933700
  47. Takuya Tamura et al. (2009). "Glial cell lineage expression of mutant ataxin-1 and huntingtin induces developmental and late-onset neuronal pathologies in Drosophila models".PMID 19165334
  48. Jeffrey N Savas et al. (2008). "Huntington's disease protein contributes to RNA-mediated gene silencing through association with Argonaute and P bodies".PMID 18669659
  49. Juliane P Caviston et al. (2007). "Huntingtin facilitates dynein/dynactin-mediated vesicle transport".PMID 17548833