This is a review about TSC1, based on 15 published articles (read how Labome selects the articles), using TSC1 antibody in all methods. It is aimed to help Labome visitors find the most suited TSC1 antibody.
TSC1 synonym: LAM; TSC; tuberous sclerosis 1 protein; tumor suppressor

Knockout validation
Invitrogenmouse monoclonal (5C8A12)
  • WB (knockout); mouse; fig 1
In order to elucidate how PLK1 regulates cell survival during mTORC1 hyperactivation, Invitrogen TSC1 antibody (Invitrogen, 37-0400) was used in western blot (knockout) on mouse samples (fig 1). Cell Cycle (2015) ncbi
Cell Signaling Technologyrabbit polyclonal
  • WB (knockout); mouse; fig 1
Cell Signaling Technology TSC1 antibody (Cell Signaling Technology, 4906S) was used in western blot (knockout) on mouse samples (fig 1). J Biol Chem (2014) ncbi
Invitrogen
mouse monoclonal (5C8A12)
  • WB (knockout); mouse; fig 1
In order to elucidate how PLK1 regulates cell survival during mTORC1 hyperactivation, Invitrogen TSC1 antibody (Invitrogen, 37-0400) was used in western blot (knockout) on mouse samples (fig 1). Cell Cycle (2015) ncbi
mouse monoclonal (5C8A12)
  • WB; human; fig 1
In order to study signaling pathways underlying HBx-induced liver tumorigenesis, Invitrogen TSC1 antibody (ZYMED, 37-0400) was used in western blot on human samples (fig 1). PLoS ONE (2012) ncbi
not indicated
  • WB; human
Zymed Laboratories anti-TSC1 antibody was used for western blot in order to show that CP1 can activate TSC2 and repress mTOR.PLoS ONE (2010) ncbi
not indicated
  • WB; human
  • IP; human
Zymed Laboratories anti-TSC1 antibody was used to perform western blot and immunoprecipitation in order to study how carboxy terminal tail of polycystin-1 inhibit mTOR via increasing the interaction between TSC2 and TSC1.Proc Natl Acad Sci U S A (2010) ncbi
mouse monoclonal (5C8A12)
  • WB; human; fig 4d
In order to suggest ARD1 inhibits the mTOR pathway, Invitrogen TSC1 antibody (Zymed, 37-0400) was used in western blot on human samples (fig 4d). Sci Signal (2010) ncbi
not indicated
  • WB; human
Invitrogen anti-TSC1 antibody was used to perform western blot in order to study the role of TSC1 missense mutations in somatic loss of function mutations in bladder cancer.Hum Mol Genet (2008) ncbi
not indicated
  • WB; human
  • IP; human
  • IC; human
Invitrogen anti-hamartin antibody was used in western blot, immunoprecipitation, and immunocytochemistry to study the interaction of hamartin with polo-like kinase 1 in human cells.Hum Mol Genet (2006) ncbi
R and D Systems
mouse monoclonal (488915)
  • IP; human
In order to identify molecular targets that suppress tumorigenesis in triple negative breast cancers, R and D Systems TSC1 antibody (R&D Systems, MAB4379) was used in immunoprecipitation on human samples. J Clin Invest (2015) ncbi
Santa Cruz Biotechnology
goat polyclonal (C-20)
  • WB; human
Santa Cruz Biotechnology TSC1 antibody (Santa Cruz Biotechnology, sc-12082) was used in western blot on human samples. Nucleic Acids Res (2014) ncbi
rabbit polyclonal (H-300)
  • WB; human
Santa Cruz anti-TSC1 was used to perform western blot in order to show that Skp2 phosphorylation by Akt1 promotes Skp2 to localize in cytoplasm and impairs APCCdh1-mediated Skp2 destructionNat Cell Biol (2009) ncbi
Epitomics
not indicated
  • IHC; human
Epitomics anti-TSC1 antibody was used to perform immunohistochemistry in order to study the role of TSC1 missense mutations in somatic loss of function mutations in bladder cancer.Hum Mol Genet (2008) ncbi
Cell Signaling Technology
rabbit monoclonal (D43E2)
  • WB; rat
Cell Signaling Technology TSC1 antibody (Cell Signaling, 6935) was used in western blot on rat samples. Mol Cell Biol (2015) ncbi
rabbit polyclonal
  • WB (knockout); mouse; fig 1
Cell Signaling Technology TSC1 antibody (Cell Signaling Technology, 4906S) was used in western blot (knockout) on mouse samples (fig 1). J Biol Chem (2014) ncbi
rabbit polyclonal
  • WB; human; fig 4d
In order to suggest ARD1 inhibits the mTOR pathway, Cell Signaling Technology TSC1 antibody (Cell Signaling Technology, 4906) was used in western blot on human samples (fig 4d). Sci Signal (2010) ncbi
not indicated
  • WB; human
Cell Signaling Technologies rabbit polyclonal anti-Tsc1 antibody was used for western blot in order to investigate the role of Tsc/mTORC1 signaling in the regulation of the quiescence and activation of primordial follicles.Hum Mol Genet (2010) ncbi
not indicated
  • WB; human
Cell Signaling Technology anti-TSC1 and anti-phospho-TSC1 antibodies were used in western blot in order to study the role of Beclin 1 in the regulation of autophagy.J Biol Chem (2009) ncbi
not indicated
  • WB; human
Cell Signaling Technology hamartin antibody was used in western blot in order to demonstrate that the brain tuberous sclerosis complex (TSC) can be caused by the loss of function of Tsc2 in radial glia.Hum Mol Genet (2009) ncbi
Sigma-Aldrich
rabbit polyclonal
  • ChIP; human; 1:25
  • EMSA; human; 1:25
  • WB; human; 1:25
Sigma-Aldrich TSC1 antibody (Sigma-Aldrich, T9574) was used in chromatin immunoprecipitation on human samples at 1:25, in EMSA on human samples at 1:25, and in western blot on human samples at 1:25. Nucleic Acids Res (2014) ncbi
Articles Reviewed
  1. Jing Zhao et al. (2015). "Death-associated protein kinase 1 promotes growth of p53-mutant cancers".PMID 26075823
  2. Matthildi Valianou et al. (2015). "Pharmacological inhibition of Polo-like kinase 1 (PLK1) by BI-2536 decreases the viability and survival of hamartin and tuberin deficient cells via induction of apoptosis and attenuation of autophagy".PMID 25565629
  3. Xiucui Ma et al. (2015). "Regulation of the transcription factor EB-PGC1α axis by beclin-1 controls mitochondrial quality and cardiomyocyte death under stress".PMID 25561470
  4. Shalmali Avinash Pradhan et al. (2014). "Evidence that TSC2 acts as a transcription factor and binds to and represses the promoter of Epiregulin".PMID 24748662
  5. Yongqiang Chen et al. (2014). "Hyperactivation of mammalian target of rapamycin complex 1 (mTORC1) promotes breast cancer progression through enhancing glucose starvation-induced autophagy and Akt signaling".PMID 24275666
  6. Chia Jui Yen et al. (2012). "Hepatitis B virus X protein upregulates mTOR signaling through IKKβ to increase cell proliferation and VEGF production in hepatocellular carcinoma".PMID 22848663
  7. Ruhee Dere et al. (2010). "Carboxy terminal tail of polycystin-1 regulates localization of TSC2 to repress mTOR".PMID 20169078
  8. Stéphanie Decherf et al. (2010). "Thyroid hormone exerts negative feedback on hypothalamic type 4 melanocortin receptor expression".PMID 20160073
  9. Hsu Ping Kuo et al. (2010). "ARD1 stabilization of TSC2 suppresses tumorigenesis through the mTOR signaling pathway".PMID 20145209
  10. Deepak Adhikari et al. (2010). "Tsc/mTORC1 signaling in oocytes governs the quiescence and activation of primordial follicles".PMID 19843540
  11. Jianrong Wang et al. (2009). "A non-canonical MEK/ERK signaling pathway regulates autophagy via regulating Beclin 1".PMID 19520853
  12. DaMing Gao et al. (2009). "Phosphorylation by Akt1 promotes cytoplasmic localization of Skp2 and impairs APCCdh1-mediated Skp2 destruction".PMID 19270695
  13. Sharon W Way et al. (2009). "Loss of Tsc2 in radial glia models the brain pathology of tuberous sclerosis complex in the mouse".PMID 19150975
  14. Louis S Pymar et al. (2008). "Bladder tumour-derived somatic TSC1 missense mutations cause loss of function via distinct mechanisms".PMID 18397877
  15. Aristotelis Astrinidis et al. (2006). "Hamartin, the tuberous sclerosis complex 1 gene product, interacts with polo-like kinase 1 in a phosphorylation-dependent manner".PMID 16339216