This is a review about Smad4, based on 33 published articles (read how Labome selects the articles), using Smad4 antibody in all methods. It is aimed to help Labome visitors find the most suited Smad4 antibody.
Smad4 synonym: DPC4; JIP; MADH4; MYHRS; MAD homolog 4; SMAD, mothers against DPP homolog 4; deleted in pancreatic carcinoma locus 4; deletion target in pancreatic carcinoma 4; mothers against decapentaplegic, Drosophila, homolog of, 4

Santa Cruz Biotechnology
mouse monoclonal (B-8)
  • IHC-P; mouse
Santa Cruz Biotechnology Smad4 antibody (Santa Cruz, sc-7966) was used in immunohistochemistry - paraffin section on mouse samples. Reprod Sci (2016) ncbi
mouse monoclonal (B-8)
  • WB; human
In order to evaluate the effect of aspirin in mesenchymal to epithelial transition, Santa Cruz Biotechnology Smad4 antibody (Santa Cruz Biotechnologies, sc-7966) was used in western blot on human samples. Lab Invest (2015) ncbi
mouse monoclonal (B-8)
  • WB; human
Santa Cruz Biotechnology Smad4 antibody (Santa Cruz Biotechnology, sc-7966) was used in western blot on human samples. Biochim Biophys Acta (2015) ncbi
mouse monoclonal (B-8)
  • IHC; human; 1:400
Santa Cruz Biotechnology Smad4 antibody (Santa Cruz Biotechnology, sc-7966) was used in immunohistochemistry on human samples at 1:400. Br J Cancer (2015) ncbi
mouse monoclonal (B-8)
  • ChIP; mouse; 2.0 ug
In order to determine how TGF-beta1-induced FGF-2 expression is regulated in prostate stromal fibroblasts and mouse embryo fibroblasts null for Smad2 and Smad3, Santa Cruz Biotechnology Smad4 antibody (Santa Cruz Biotechnology, B8) was used in chromatin immunoprecipitation on mouse samples at 2.0 ug. Am J Clin Exp Urol (2014) ncbi
mouse monoclonal (B-8)
  • WB; mouse; 1:1000; fig 1c
In order to show that Basonuclin-1 regulates TGF-beta1-induced epithelial dedifferentiation of mammary epithelial cells, Santa Cruz Biotechnology Smad4 antibody (Santa Cruz, sc-7966) was used in western blot on mouse samples at 1:1000 (fig 1c). Oncogene (2015) ncbi
mouse monoclonal (B-8)
  • IHC-P; human; 1:200
Santa Cruz Biotechnology Smad4 antibody (Santa Cruz, sc-7966) was used in immunohistochemistry - paraffin section on human samples at 1:200. Hum Pathol (2014) ncbi
mouse monoclonal (B-8)
  • WB; human
  • IC; human
Santa Cruz Biotechnology Smad4 antibody (Santa Cruz Biotechnology, sc-7966) was used in western blot on human samples and in immunocytochemistry on human samples. J Biol Chem (2014) ncbi
rabbit polyclonal (H-552)
  • ChIP; human
  • WB; human
Santa Cruz Biotechnology Smad4 antibody (Santa Cruz, sc-7154) was used in chromatin immunoprecipitation on human samples and in western blot on human samples. J Biol Chem (2013) ncbi
mouse monoclonal (B-8)
  • ChIP; human
  • IP; human
  • IP; mouse
  • WB; human
  • WB; mouse
Santa Cruz Biotechnology Smad4 antibody (Santa Cruz, sc-7966x) was used in chromatin immunoprecipitation on human samples, in immunoprecipitation on human samples and on mouse samples, and in western blot on human samples and on mouse samples. J Biol Chem (2013) ncbi
not indicated
  • WB; human
Santa Cruz Biotechnology anti-Smad4 was used to perform western blot in order to study the roles of RBBP9 as a tumor-associated serine hydrolase to pancreatic neoplasiaProc Natl Acad Sci U S A (2010) ncbi
not indicated
  • WB; human
Santa Cruz Biotechnology Smad4 monoclonal antibody was used in western blot in order to investigate the role of BMP9 signalling via ALK2/Smad1/Smad4 pathway in ovarian cancer.Cancer Res (2009) ncbi
not indicated
  • WB; human
Santa Cruz Biotechnology Inc anti-Smad4 antibody was used for western blot in order to investigate the role of TGFB1 in the regulation of Mdm2 expression and late-stage breast cancer metastasis.J Clin Invest (2010) ncbi
not indicated
  • WB; human
Santa Cruz Biotechnology anti-Smad4 antibody was used for western blot to study the suppressing role of human FHL1 family members in the modulation of tumor cell growth through a TGF-beta like signaling pathway.J Clin Invest (2009) ncbi
not indicated
  • ChIP; human
Santa Cruz Biotechnology rabbit anti-Smad4 antibody was used to carry out chromatin immunoprecipitation assays in order to investigate the roles for HMGA2 and Smads in the regulation of SNAIL1 expression during epithelial-mesenchymal transition.J Biol Chem (2008) ncbi
not indicated
  • WB; human
Santa Cruz rabbit anti-Smad4(H-552; 1:1,000) antibody was used to perform western blot in order to study the dynamical regulation of TGF-beta signaling during late lung development in the human and mouse.Dev Dyn (2008) ncbi
mouse monoclonal (B-8)
  • WB; human
Santa Cruz Biotechnology mouse anti-Smad4 monoclonal antibody (Catalog #: sc-7966) was used to perform western blot assays in order to investigate the molecular regulation mechanisms of Smad4 in the process of normal pancreas development and pancreatic ductal adenocarcinomas (PDAC) growth.Adv Drug Deliv Rev (2006) ncbi
not indicated
  • WB; human
Santa Cruz Biotechnologies anti-Smad4 antibody was used in western blot to study COLO-357 cell growth inhibition by TGF beta1J Biol Chem (2005) ncbi
not indicated
  • WB; human
  • IP; human
  • ChIP; human
Santa Cruz monoclonal Smad4 (B-8) and polyclonal Smad4 (G-20) antibodies were used in western blot, immunoprecipitation, and ChIP assay to study transcriptional repression of smad4J Biol Chem (2005) ncbi
Abcam
rabbit monoclonal (EP618Y)
  • WB; mouse; fig 2
Abcam Smad4 antibody (Abcam, EP618Y) was used in western blot on mouse samples (fig 2). Cell (2015) ncbi
rabbit monoclonal (EP618Y)
  • WB; human
Abcam Smad4 antibody (Abcam, ab40759) was used in western blot on human samples. World J Gastroenterol (2014) ncbi
rabbit monoclonal (EP618Y)
  • IHC-P; human; 1:100
Abcam Smad4 antibody (Abcam, ab40759) was used in immunohistochemistry - paraffin section on human samples at 1:100. Histopathology (2014) ncbi
rabbit monoclonal (EP618Y)
  • WB; mouse; 1:1000
In order to examine the functions of Smad proteins during cerebellum development, Abcam Smad4 antibody (Abcam, ab40759) was used in western blot on mouse samples at 1:1000. Mol Cell Biol (2013) ncbi
EMD Millipore
rabbit monoclonal (EP618Y)
  • IHC-P; human; 1:100
In order to screen a panel of 33 proteins to establish a set of biomarkers that predict the agressiveness of prostate cancer, EMD Millipore Smad4 antibody (Millipore, 04-1033) was used in immunohistochemistry - paraffin section on human samples at 1:100. BMC Cancer (2014) ncbi
rabbit polyclonal
  • WB; rat
In order to study the effect of rosiglitazone treatment on the transforming growth factor-beta/SMAD signaling pathway in white adipose tissue of diabetic rats, EMD Millipore Smad4 antibody (Millipore, ABE21) was used in western blot on rat samples. Obesity (Silver Spring) (2014) ncbi
rabbit monoclonal (EP618Y)
  • IHC-Free; mouse; 1:100
In order to examine the functions of Smad proteins during cerebellum development, EMD Millipore Smad4 antibody (Millipore, 04-1033) was used in immunohistochemistry - free floating section on mouse samples at 1:100. Mol Cell Biol (2013) ncbi
rabbit monoclonal (EP618Y)
  • WB; human; 1:1000
  • WB; mouse; 1:1000
EMD Millipore Smad4 antibody (Millipore, 04-1033) was used in western blot on human samples at 1:1000 and on mouse samples at 1:1000. J Appl Oral Sci (2012) ncbi
R and D Systems
goat polyclonal
  • WB; human
  • IC; human
R and D Systems Smad4 antibody (R&D, AF2097) was used in western blot on human samples and in immunocytochemistry on human samples. Cell Res (2014) ncbi
Cell Signaling Technology
rabbit polyclonal
  • WB; human; 1:1000; fig 2b
Cell Signaling Technology Smad4 antibody (Cell Signalling, 9515) was used in western blot on human samples at 1:1000 (fig 2b). Nat Commun (2015) ncbi
rabbit polyclonal
  • WB; human; fig 1
Cell Signaling Technology Smad4 antibody (Cell Signaling, 9515) was used in western blot on human samples (fig 1). J Biol Chem (2015) ncbi
rabbit polyclonal
  • WB; mouse
In order to identify DUBA as a suppressor of IL-17 production in T cells, Cell Signaling Technology Smad4 antibody (Cell Signaling, 9515S) was used in western blot on mouse samples. Nature (2015) ncbi
rabbit polyclonal
  • WB; rat; fig 4
Cell Signaling Technology Smad4 antibody (Cell Signaling Technology, 9515) was used in western blot on rat samples (fig 4). Biochem Pharmacol (2015) ncbi
rabbit polyclonal
  • WB; human; 1:1000
In order to demonstrate that baicalein inhibits metastasis of cancer cells via inactivation of TGF-beta/Smad4 signaling, Cell Signaling Technology Smad4 antibody (Cell Signaling Technology, 9515) was used in western blot on human samples at 1:1000. Mol Med Rep (2014) ncbi
not indicated
  • WB; human
Cell Signaling anti-Smad-4 antibody (no. 9515) was used in western blot to test a hypothesis that activin C antagonizes the effects of activin A and its overexpression leads to pathologies.Am J Pathol (2009) ncbi
Articles Reviewed
  1. K Bacallao et al. (2016). "Levels of Regulatory Proteins Associated With Cell Proliferation in Endometria From Untreated Patients Having Polycystic Ovarian Syndrome With and Without Endometrial Hyperplasia".PMID 26239387
  2. Jason S L Yu et al. (2015). "PI3K/mTORC2 regulates TGF-β/Activin signalling by modulating Smad2/3 activity via linker phosphorylation".PMID 25998442
  3. Gargi Maity et al. (2015). "Aspirin blocks growth of breast tumor cells and tumor-initiating cells and induces reprogramming factors of mesenchymal to epithelial transition".PMID 25867761
  4. Sho Aki et al. (2015). "Phosphatidylinositol 3-kinase class II α-isoform PI3K-C2α is required for transforming growth factor β-induced Smad signaling in endothelial cells".PMID 25614622
  5. Sylvia F Boj et al. (2015). "Organoid models of human and mouse ductal pancreatic cancer".PMID 25557080
  6. Patryk Krzeminski et al. (2015). "Insights into epigenetic regulation of microRNA-155 expression in multiple myeloma".PMID 25497370
  7. Sascha Rutz et al. (2015). "Deubiquitinase DUBA is a post-translational brake on interleukin-17 production in T cells".PMID 25470037
  8. Tianjiao Xu et al. (2015). "Ferulic acid suppresses activation of hepatic stellate cells through ERK1/2 and Smad signaling pathways in vitro".PMID 25449600
  9. P W Voorneveld et al. (2015). "The BMP pathway either enhances or inhibits the Wnt pathway depending on the SMAD4 and p53 status in CRC".PMID 25393365
  10. Douglas W Strand et al. (2014). "TGF-β induction of FGF-2 expression in stromal cells requires integrated smad3 and MAPK pathways".PMID 25374926
  11. Ru Yi Chen et al. (2014). "Effect of oridonin-mediated hallmark changes on inflammatory pathways in human pancreatic cancer (BxPC-3) cells".PMID 25356049
  12. Fenglin Chen et al. (2014). "Baicalein inhibits migration and invasion of gastric cancer cells through suppression of the TGF-β signaling pathway".PMID 25109410
  13. Kazunobu Isogaya et al. (2014). "A Smad3 and TTF-1/NKX2-1 complex regulates Smad4-independent gene expression".PMID 25060702
  14. Anthony E Rizzardi et al. (2014). "Evaluation of protein biomarkers of prostate cancer aggressiveness".PMID 24708576
  15. A Feuerborn et al. (2015). "Basonuclin-1 modulates epithelial plasticity and TGF-β1-induced loss of epithelial cell integrity".PMID 24662832
  16. Marie Soleil Beaudoin et al. (2014). "Novel effects of rosiglitazone on SMAD2 and SMAD3 signaling in white adipose tissue of diabetic rats".PMID 24500776
  17. Xuefeng Zhang et al. (2014). "Pancreatic ductal adenocarcinoma with autoimmune pancreatitis-like histologic and immunohistochemical features".PMID 24457081
  18. Yili Feng et al. (2014). "Zinc finger protein 451 is a novel Smad corepressor in transforming growth factor-β signaling".PMID 24324267
  19. Jianwei Sun et al. (2013). "GATA3 transcription factor abrogates Smad4 transcription factor-mediated fascin overexpression, invadopodium formation, and breast cancer cell invasion".PMID 24235142
  20. Cuiping Wang et al. (2014). "Distinguishing adrenal cortical carcinomas and adenomas: a study of clinicopathological features and biomarkers".PMID 24102952
  21. Masayo Sakaki-Yumoto et al. (2013). "Smad2 is essential for maintenance of the human and mouse primed pluripotent stem cell state".PMID 23649632
  22. Ka Kui Tong et al. (2013). "Common partner Smad-independent canonical bone morphogenetic protein signaling in the specification process of the anterior rhombic lip during cerebellum development".PMID 23459943
  23. Ariadne Cristiane Cabral Cruz et al. (2012). "Addition of bone morphogenetic protein type 2 to ascorbate and β-glycerophosphate supplementation did not enhance osteogenic differentiation of human adipose-derived stem cells".PMID 23329244
  24. David J Shields et al. (2010). "RBBP9: a tumor-associated serine hydrolase activity required for pancreatic neoplasia".PMID 20080647
  25. Blanca Herrera et al. (2009). "Autocrine bone morphogenetic protein-9 signals through activin receptor-like kinase-2/Smad1/Smad4 to promote ovarian cancer cell proliferation".PMID 19996292
  26. Shinako Araki et al. (2010). "TGF-beta1-induced expression of human Mdm2 correlates with late-stage metastatic breast cancer".PMID 19955655
  27. Lihua Ding et al. (2009). "Human four-and-a-half LIM family members suppress tumor cell growth through a TGF-beta-like signaling pathway".PMID 19139564
  28. Elspeth Gold et al. (2009). "Activin C antagonizes activin A in vitro and overexpression leads to pathologies in vivo".PMID 19095948
  29. Sylvie Thuault et al. (2008). "HMGA2 and Smads co-regulate SNAIL1 expression during induction of epithelial-to-mesenchymal transition".PMID 18832382
  30. Miguel A Alejandre-Alcázar et al. (2008). "TGF-beta signaling is dynamically regulated during the alveolarization of rodent and human lungs".PMID 18095342
  31. Anna Sergeeva et al. (2006). "Display technologies: application for the discovery of drug and gene delivery agents".PMID 17123658
  32. Nichole Boyer Arnold et al. (2005). "Smad7 abrogates transforming growth factor-beta1-mediated growth inhibition in COLO-357 cells through functional inactivation of the retinoblastoma protein".PMID 15811853
  33. Che Chang Chang et al. (2005). "Daxx mediates the small ubiquitin-like modifier-dependent transcriptional repression of Smad4".PMID 15637079