This is a review about Smad2, based on 114 published articles (read how Labome selects the articles), using Smad2 antibody in all methods. It is aimed to help Labome visitors find the most suited Smad2 antibody.
Smad2 synonym: JV18; JV18-1; MADH2; MADR2; hMAD-2; hSMAD2; MAD homolog 2; SMAD, mothers against DPP homolog 2; Sma- and Mad-related protein 2; mother against DPP homolog 2

EMD Millipore
rabbit polyclonal
  • IHC; mouse
In order to assess the use of 3D1 mAb for treating Nodal expressing cancers, EMD Millipore Smad2 antibody (Millipore, AB3849) was used in immunohistochemistry on mouse samples. Oncotarget (2015) ncbi
rabbit polyclonal
  • WB; human
In order to assess the use of 3D1 mAb for treating Nodal expressing cancers, EMD Millipore Smad2 antibody (Millipore, 07-408) was used in western blot on human samples. Oncotarget (2015) ncbi
rabbit polyclonal
  • IC; mouse; 1:500; fig s4
In order to report how nuclear pore complex remodeling regulates astrocyte-neuronal communication, EMD Millipore Smad2 antibody (Millipore, ab3849) was used in immunocytochemistry on mouse samples at 1:500 (fig s4). Nat Neurosci (2015) ncbi
rabbit polyclonal
  • IHC; human; fig 3
EMD Millipore Smad2 antibody (Millipore, AB3849) was used in immunohistochemistry on human samples (fig 3). J Biol Chem (2015) ncbi
rabbit polyclonal
  • WB; mouse
EMD Millipore Smad2 antibody (Millipore, AB3849) was used in western blot on mouse samples. Stem Cells (2015) ncbi
rabbit polyclonal
  • IHC-F; mouse; 1:200
In order to study the role of astrocytic TGF-beta during central nervous system Toxoplasma infection, EMD Millipore Smad2 antibody (Millipore, AB3849) was used in immunohistochemistry - frozen section on mouse samples at 1:200. J Immunol (2014) ncbi
rabbit polyclonal
  • IHC-F; mouse; 1:1000
  • WB; mouse; 1:200
EMD Millipore Smad2 antibody (Millipore, AB3849) was used in immunohistochemistry - frozen section on mouse samples at 1:1000 and in western blot on mouse samples at 1:200. Glia (2014) ncbi
rabbit polyclonal
  • WB; human
EMD Millipore Smad2 antibody (Millipore, AB3849) was used in western blot on human samples. Br J Cancer (2014) ncbi
rabbit polyclonal
  • WB; human
EMD Millipore Smad2 antibody (Millipore, 07-408) was used in western blot on human samples. Br J Cancer (2014) ncbi
rabbit polyclonal
  • IHC; pig; 1:500
EMD Millipore Smad2 antibody (Millipore, AB3849) was used in immunohistochemistry on pig samples at 1:500. Dev Biol (2014) ncbi
rabbit polyclonal
  • WB; rat; 1:1000
EMD Millipore Smad2 antibody (Upstate, 07408) was used in western blot on rat samples at 1:1000. J Neurochem (2014) ncbi
rabbit polyclonal
  • WB; rat; 1:1000
EMD Millipore Smad2 antibody (Millipore, Ab3849) was used in western blot on rat samples at 1:1000. J Neurochem (2014) ncbi
rabbit polyclonal
  • IHC-P; mouse; 1:150
  • WB; African green monkey; 1:500
  • WB; mouse; 1:500
In order to evaluate the role of transforming growth factor-beta3 in inflammation and glucose tolerance, EMD Millipore Smad2 antibody (Millipore, AB3849) was used in immunohistochemistry - paraffin section on mouse samples at 1:150 and in western blot on African green monkey samples at 1:500 and on mouse samples at 1:500. J Biol Chem (2013) ncbi
rabbit monoclonal (A5S)
  • WB; human
  • WB; mouse
  • WB; rat
EMD Millipore Smad2 antibody (Millipore, A5S) was used in western blot on human samples, on mouse samples, and on rat samples. Biol Cell (2013) ncbi
not indicated
  • IHC; human
Millipore rabbit monoclonal antibody against human and mouse phosphorylated Smad2 (clone A5s) was used in immunohistochemistry to investigate the role of transforming growth factor Beta signaling in the regulation of tumor growth.J Natl Cancer Inst (2009) ncbi
not indicated
  • WB; human
Calbiochem rabbit anti-P-Smad2 antibody was used in western blot in order to demostrate that the target gene expression can be promoted through Smad2/3 activation.PLoS ONE (2009) ncbi
not indicated
  • IHC; human
  • WB; human
Upstate rabbit anti-phospho-Smad2(Ser465/467; catalog no. 05-953; 1:50) antibody was used to perform immunohistochemistry and western blot in order to study the dynamical regulation of TGF-beta signaling during late lung development in the human and mouse.Dev Dyn (2008) ncbi
rabbit polyclonal
  • WB; human
Chemicon International.Inc anti-phospho-Smad2 rabbit polyclonal antibody(AB3849) was used to perform western blot assays in order to investigate the molecular regulation mechanisms of Smad4 in the process of normal pancreas development and pancreatic ductal adenocarcinomas (PDAC) growth.Adv Drug Deliv Rev (2006) ncbi
not indicated
  • WB; human
Upstate Biotechnologies phospho-Smad2 antibody was used in western blot to study COLO-357 cell growth inhibition by TGF beta1J Biol Chem (2005) ncbi
not indicated
  • IHC; human
Upstate anti-Smad2/3 antibodies were used in immunohistochemistry to study chondrogenesis Induction by Smad3J Biol Chem (2005) ncbi
Invitrogen
rabbit polyclonal
  • WB; human
In order to assess the use of 3D1 mAb for treating Nodal expressing cancers, Invitrogen Smad2 antibody (Life Technologies, 44-244G) was used in western blot on human samples. Oncotarget (2015) ncbi
rabbit polyclonal
  • WB; mouse
In order to determine how TGF-beta1-induced FGF-2 expression is regulated in prostate stromal fibroblasts and mouse embryo fibroblasts null for Smad2 and Smad3, Invitrogen Smad2 antibody (Zymed, 51-1300) was used in western blot on mouse samples. Am J Clin Exp Urol (2014) ncbi
rabbit polyclonal
  • WB; mouse
In order to demonstrate that bone morphogenetic protein signaling, acting through Smad1, Smad5 and Smad8, is the fundamental hypertrophic signal in mice, Invitrogen Smad2 antibody (Life Technologies, 44-244G) was used in western blot on mouse samples. Nat Genet (2013) ncbi
rabbit polyclonal
  • WB; African green monkey; 1:500
  • WB; mouse; 1:500
In order to evaluate the role of transforming growth factor-beta3 in inflammation and glucose tolerance, Invitrogen Smad2 antibody (Invitrogen, 51-1300) was used in western blot on African green monkey samples at 1:500 and on mouse samples at 1:500. J Biol Chem (2013) ncbi
rabbit polyclonal
  • IHC-P; human; 1:100
In order to study the expression and prognostic value of Wnt and TGF-beta pathway components and key adrenal transcription factors in adrenocortical tumors, Invitrogen Smad2 antibody (Zymed, 51-1300) was used in immunohistochemistry - paraffin section on human samples at 1:100. Pathol Res Pract (2013) ncbi
rabbit polyclonal
  • WB; human; fig 2
In order to investigate the role of cell surface CR-1 expression in human melanoma cells, Invitrogen Smad2 antibody (Invitrogen, 44244G) was used in western blot on human samples (fig 2). Cell Cycle (2013) ncbi
rabbit polyclonal
  • WB; mouse; fig 7
In order to demonstrate that cycloheximide produces inhibin-like effects in gonadotropes by preventing de novo synthesis of ACVR2, Invitrogen Smad2 antibody (Zymed, 51-1300) was used in western blot on mouse samples (fig 7). Cell Signal (2013) ncbi
rabbit polyclonal
  • WB; mouse; 1:1000
In order to study the role of Smad3 in the effect of TGF-beta in the mammary epithelium, Invitrogen Smad2 antibody (Zymed, 51-1300) was used in western blot on mouse samples at 1:1000. Mol Cancer Res (2012) ncbi
rabbit polyclonal
  • WB; human; fig 1
In order to study the relationship between bone morphogenetic protein and TGF-beta, Invitrogen Smad2 antibody (Life Technologies, 511300) was used in western blot on human samples (fig 1). Mol Cell Biol (2012) ncbi
rabbit polyclonal
  • WB; human; fig 3
In order to study the effects of Smad4 loss on the levels of beta-catenin messenger RNA and Wnt signaling, Invitrogen Smad2 antibody (Zymed Laboratories, 51-1300) was used in western blot on human samples (fig 3). Gastroenterology (2012) ncbi
rabbit polyclonal
  • WB; human; fig 4
In order to assess the effects of beta-catenin modulation on tubular epithelial-mesenchymal transition, Invitrogen Smad2 antibody (Invitrogen, 51-1300) was used in western blot on human samples (fig 4). J Am Soc Nephrol (2011) ncbi
rabbit polyclonal
  • WB; human
In order to demonstrate that SMAD and FOXL2 form a complex that activates transcription, Invitrogen Smad2 antibody (Zymed, 51-1300) was used in western blot on human samples. Endocrinology (2010) ncbi
not indicated
  • IC; human
Zymed Laboratories Smad2 was used to be dually phosphorylated at Ser465 and Ser467 in order to study the mechanism of activin receptor signaling regulating prostatic epithelial cell adhesion and viabilityNeoplasia (2009) ncbi
not indicated
  • WB; human
Zymed Laboratories rabbit anti-Smad2 antibody was used in western blot in order to demostrate that the target gene expression can be promoted through Smad2/3 activation.PLoS ONE (2009) ncbi
not indicatedInvitrogen siRNA for Smad2 (sense, 5-AAUGGAGUGAGUAUAGUCAUCCAGA-3) was used for RNAi in order to study the roles for ETS1 and TFAP2A in the transcription induced by TGF-beta-Smad pathways.Mol Cell Biol (2009) ncbi
rabbit polyclonal
  • WB; human
Zymed rabbit anti-Smad2(51-1300; 1:1,000) antibody was used to perform western blot in order to study the dynamical regulation of TGF-beta signaling during late lung development in the human and mouse.Dev Dyn (2008) ncbi
not indicated
  • WB; human
Zymed Laboratories Smad2 antibody was used in western blot to study the role of disintegrin and metalloproteinase ADAM12 in TGF-beta signaling through interaction with the type II receptorJ Cell Biol (2007) ncbi
not indicated
  • WB; human
Biosource anti-phospho-Smad2 (Ser465/467) antibody was used in western blot to study endofin as a Smad anchor for receptor activation in BMP signalingJ Cell Sci (2007) ncbi
not indicated
  • IC; human
Zymed Laboratories rabbit anti-Smad2/Smad3 antibody was used in immunocytochemistry to study COLO-357 cell growth inhibition by TGF beta1J Biol Chem (2005) ncbi
not indicated
  • WB; human
Smad2 antibody was used in western blot to study activation of Smad2 by ALK5J Biol Chem (2005) ncbi
rabbit polyclonal
  • WB; African green monkey; fig 1
In order to develop functional epitope-tagged TGF-beta proteins, Invitrogen Smad2 antibody (Zymed, Cat #51-1300) was used in western blot on African green monkey samples (fig 1). J Immunol Methods (2002) ncbi
Santa Cruz Biotechnology
goat polyclonal (N-19)
  • WB; rat; 1:100
Santa Cruz Biotechnology Smad2 antibody (Santa Cruz Biotechnology, sc-6032) was used in western blot on rat samples at 1:100. Neural Regen Res (2015) ncbi
goat polyclonal (E-20)
  • ChIP; mouse; 2.0 ug
In order to determine how TGF-beta1-induced FGF-2 expression is regulated in prostate stromal fibroblasts and mouse embryo fibroblasts null for Smad2 and Smad3, Santa Cruz Biotechnology Smad2 antibody (Santa Cruz Biotechnology, E20) was used in chromatin immunoprecipitation on mouse samples at 2.0 ug. Am J Clin Exp Urol (2014) ncbi
mouse monoclonal (C-8)
  • WB; human; 1:500
Santa Cruz Biotechnology Smad2 antibody (Santa Cruz, SC-133098) was used in western blot on human samples at 1:500. Growth Factors (2015) ncbi
goat polyclonal (N-19)
  • WB; human
Santa Cruz Biotechnology Smad2 antibody (Santa Cruz Biotechnology, sc-6032) was used in western blot on human samples. Int J Oncol (2014) ncbi
rabbit polyclonal (Thr 220)
  • WB; human
Santa Cruz Biotechnology Smad2 antibody (Santa Cruz Biotechnology, sc-135644) was used in western blot on human samples. Int J Oncol (2014) ncbi
mouse monoclonal (C-8)
  • WB; human
Santa Cruz Biotechnology Smad2 antibody (Santa Cruz Biotechnology, SC-133098) was used in western blot on human samples. Mol Biol Cell (2014) ncbi
not indicated
  • IC; human
Santa Cruz Biotechnology mouse anti-Smad2 antibody was used to carry out immunofluorescence assays in order to investigate the role for transforming growth factor beta in regulating neuronal cell development.Cereb Cortex (2010) ncbi
not indicated
  • IC; human
Santa Cruz Biotechnology rabbit anti-phosphorylated Smad2/3 (Ser423/425) antibody (1:50) was used in immunocytochemistry in order to investigate the regulatory role of transforming growth factor-beta in valve interstitial cell function during wound repairAm J Pathol (2008) ncbi
not indicated
  • IHC; human
Santa Cruz Biotechnology rabbit polyclonal phosphorylated Smad2/3 antibdoy was used in immunohistochemistry in order to demonstrate that mice expressed a latent form of TGF-beta1 in skin keratinocytes exhibited renoprotective effects on renal fibrosis in obstructive kidney diseaseAm J Physiol Renal Physiol (2008) ncbi
not indicated
  • WB; human
Santa Cruz pSmad2/3 (Ser433/435) antibody was used in western blot in order to demonstrate that lithium suppresses Smad3/4-dependent TGF-beta-responsive gene transactivationMol Cell Neurosci (2008) ncbi
not indicated
  • WB; human
  • IP; human
Santa Cruz Biotechnology anti-Smad2 antibody was used in western blot and immunoprecipitation to investigate the regulation and mechanism of renal SnoN expression in vivo.J Am Soc Nephrol (2006) ncbi
not indicated
  • WB; human
Santa Cruz Biotechnology rabbit anti-phospho-Smad2/3 (Ser433/Ser435) antibody was used in western blot to hypoxia inhibition of adipocytogenesis in human bone marrow stromal cellsJ Biol Chem (2005) ncbi
not indicated
  • IC; human
Santa Cruz Biotechnology mouse anti-Smad1/2/3 (H-2) antibody was used in immunocytochemistry to study the role of internalization in transforming growth factor beta 1-induced smad2 association with smad anchor for receptor activation (SARA) and smad2-dependent signaling in human mesangial cellsJ Biol Chem (2005) ncbi
Abcam
rabbit polyclonal
  • WB; human
Abcam Smad2 antibody (Abcam, ab53100) was used in western blot on human samples. World J Gastroenterol (2014) ncbi
rabbit monoclonal (EP567Y)
  • WB; human
Abcam Smad2 antibody (Abcam, ab33875) was used in western blot on human samples. World J Gastroenterol (2014) ncbi
rabbit polyclonal
  • ICW; human
In order to examine the mechanism of the inhibin and activin signalling pathway in cancers, Abcam Smad2 antibody (Abcam, 53100) was used in In-Cell Western on human samples. Mol Hum Reprod (2014) ncbi
rabbit polyclonal
  • ICW; human
In order to examine the mechanism of the inhibin and activin signalling pathway in cancers, Abcam Smad2 antibody (Abcam, 47083) was used in In-Cell Western on human samples. Mol Hum Reprod (2014) ncbi
rabbit monoclonal (EP567Y)
  • IHC; mouse
Abcam Smad2 antibody (Abcam, ab33875) was used in immunohistochemistry on mouse samples. Cardiovasc Res (2014) ncbi
rabbit monoclonal (EP784Y)
  • WB; human
Abcam Smad2 antibody (Abcam, ab40855) was used in western blot on human samples. PLoS ONE (2013) ncbi
rabbit polyclonal
  • IC; mouse; 1:100
Abcam Smad2 antibody (Abcam, AB63576) was used in immunocytochemistry on mouse samples at 1:100. Development (2012) ncbi
R and D Systems
goat polyclonal
  • WB; mouse; 1:2000
R and D Systems Smad2 antibody (R&D Systems, AF3797) was used in western blot on mouse samples at 1:2000. Cell Physiol Biochem (2014) ncbi
Epitomics
not indicated
  • WB; human
Epitomics anti-Smad2 antibody was used to carry out western blot analysis in order to investigate the role for Smurf2 in promoting breast cancer metastasis.Cancer Res (2009) ncbi
not indicated
  • WB; human
Epitomics rabbit monoclonal anti-Smad1 was used to perform western blot in order to show the mechanism that two functions of TGF become un-coupled during cancer development.Cancer Res (2008) ncbi
Cell Signaling Technology
rabbit monoclonal (D7G7)
  • WB; human; 1:1000; fig 4
Cell Signaling Technology Smad2 antibody (Cell Signaling Technology, 8685) was used in western blot on human samples at 1:1000 (fig 4). PLoS Med (2015) ncbi
rabbit monoclonal (138D4)
  • WB; mouse; fig 1
In order to study the role of JNK signaling in the epithelial to mesenchymal transition, Cell Signaling Technology Smad2 antibody (Cell signaling, 3108) was used in western blot on mouse samples (fig 1). EMBO J (2015) ncbi
rabbit monoclonal (86F7)
  • WB; human; fig 7
Cell Signaling Technology Smad2 antibody (Cell Signaling Technology, 3122) was used in western blot on human samples (fig 7). J Biol Chem (2015) ncbi
rabbit monoclonal (138D4)
  • WB; human; fig 7
Cell Signaling Technology Smad2 antibody (Cell Signaling Technology, 3108) was used in western blot on human samples (fig 7). J Biol Chem (2015) ncbi
rabbit polyclonal
  • WB; mouse; fig 2.a,b
In order to report how nuclear pore complex remodeling regulates astrocyte-neuronal communication, Cell Signaling Technology Smad2 antibody (Cell Signaling, 3101) was used in western blot on mouse samples (fig 2.a,b). Nat Neurosci (2015) ncbi
mouse monoclonal (L16D3)
  • WB; mouse; fig 2.a,b
In order to report how nuclear pore complex remodeling regulates astrocyte-neuronal communication, Cell Signaling Technology Smad2 antibody (Cell Signaling, 3103) was used in western blot on mouse samples (fig 2.a,b). Nat Neurosci (2015) ncbi
rabbit polyclonal
  • IHC-P; mouse; 1:100
In order to demonstrate that enhanced BMP signaling through constitutively active ACVR1 in palatal epithelium causes submucous cleft palate in mice, Cell Signaling Technology Smad2 antibody (Cell signaling, 3101) was used in immunohistochemistry - paraffin section on mouse samples at 1:100. Dev Biol (2015) ncbi
rabbit monoclonal (86F7)
  • WB; human; 1:1000
Cell Signaling Technology Smad2 antibody (Cell Signaling, 86F7) was used in western blot on human samples at 1:1000. PLoS ONE (2015) ncbi
rabbit monoclonal (86F7)
  • WB; human; 1:1000; fig 2b
  • IP; human; 1:1000; fig 2.d,e
Cell Signaling Technology Smad2 antibody (Cell Signalling, 3122) was used in western blot on human samples at 1:1000 (fig 2b) and in immunoprecipitation on human samples at 1:1000 (fig 2.d,e). Nat Commun (2015) ncbi
rabbit monoclonal (138D4)
  • IHC-P; mouse; 1:100; fig 2
  • WB; mouse; fig 2
In order to test if a leucine-serine-lysine-leucine peptide promotes liver regeneration, Cell Signaling Technology Smad2 antibody (Cell Signaling Technology, 138D4) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 2) and in western blot on mouse samples (fig 2). Br J Surg (2015) ncbi
rabbit polyclonal
  • WB; mouse
In order to elucidate the role of SMAD7 in muscle, Cell Signaling Technology Smad2 antibody (Cell Signaling Technology, 3101) was used in western blot on mouse samples. J Physiol (2015) ncbi
rabbit monoclonal (D43B4)
  • WB; mouse
In order to demonstrate that miR-200 suppresses TGF-beta/BMP signaling, promotes epithelial gene expression, and suppresses cell invasion by regulating a network of genes, Cell Signaling Technology Smad2 antibody (Cell Signaling, 5339) was used in western blot on mouse samples. Oncogene (2016) ncbi
rabbit monoclonal (138D4)
  • WB; mouse; 1:500
In order to characterize mice harboring a disrupted allele for the Lem2 gene, Cell Signaling Technology Smad2 antibody (CST, 138D4) was used in western blot on mouse samples at 1:500. PLoS ONE (2015) ncbi
rabbit monoclonal (D43B4)
  • WB; human
In order to report that L3MBTL1 represses the ability of stem cells to drive hematopoietic-specific transcriptional programs via SMAD5 and impairing its recruitment to target regulatory regions, Cell Signaling Technology Smad2 antibody (Cell Signaling, 5339P) was used in western blot on human samples. Stem Cell Reports (2015) ncbi
rabbit monoclonal (138D4)
  • WB; human
In order to report that L3MBTL1 represses the ability of stem cells to drive hematopoietic-specific transcriptional programs via SMAD5 and impairing its recruitment to target regulatory regions, Cell Signaling Technology Smad2 antibody (Cell Signaling, 3108S) was used in western blot on human samples. Stem Cell Reports (2015) ncbi
rabbit polyclonal
  • WB; human; fig 1
Cell Signaling Technology Smad2 antibody (Cell Signaling, 3101) was used in western blot on human samples (fig 1). J Biol Chem (2015) ncbi
rabbit monoclonal (D43B4)
  • WB; human; 1:1000
Cell Signaling Technology Smad2 antibody (Cell Signaling, 5339S) was used in western blot on human samples at 1:1000. PLoS ONE (2015) ncbi
rabbit monoclonal (138D4)
  • WB; human; 1:1000
Cell Signaling Technology Smad2 antibody (Cell Signaling, 3108S) was used in western blot on human samples at 1:1000. PLoS ONE (2015) ncbi
rabbit monoclonal (D43B4)
  • IC; human; 1:100
Cell Signaling Technology Smad2 antibody (Cell Signaling, 5339) was used in immunocytochemistry on human samples at 1:100. Exp Cell Res (2015) ncbi
rabbit polyclonal
  • WB; human
Cell Signaling Technology Smad2 antibody (Cell Signalin, 3101) was used in western blot on human samples. J Biol Chem (2015) ncbi
rabbit polyclonal
  • WB; mouse
Cell Signaling Technology Smad2 antibody (Cell Signaling, 3101) was used in western blot on mouse samples. Biochim Biophys Acta (2015) ncbi
mouse monoclonal (L16D3)
  • WB; mouse
Cell Signaling Technology Smad2 antibody (Cell Signaling, 3103) was used in western blot on mouse samples. Biochim Biophys Acta (2015) ncbi
rabbit polyclonal
  • WB; mouse
In order to identify DUBA as a suppressor of IL-17 production in T cells, Cell Signaling Technology Smad2 antibody (Cell Signaling, 5678S) was used in western blot on mouse samples. Nature (2015) ncbi
rabbit polyclonal
  • WB; mouse
In order to identify DUBA as a suppressor of IL-17 production in T cells, Cell Signaling Technology Smad2 antibody (Cell Signaling, 3101S) was used in western blot on mouse samples. Nature (2015) ncbi
rabbit polyclonal
  • WB; mouse
In order to determine how TGF-beta1-induced FGF-2 expression is regulated in prostate stromal fibroblasts and mouse embryo fibroblasts null for Smad2 and Smad3, Cell Signaling Technology Smad2 antibody (Cell Signaling, 3101) was used in western blot on mouse samples. Am J Clin Exp Urol (2014) ncbi
rabbit monoclonal (138D4)
  • WB; rat; 1:1000
In order to show that fibroblast growth factors and bone morphogenetic proteins contribute to astrocyte development, Cell Signaling Technology Smad2 antibody (Cell Signaling Technology, 3108) was used in western blot on rat samples at 1:1000. PLoS ONE (2014) ncbi
rabbit monoclonal (138D4)
  • IHC-P; human
  • WB; human; 1:1000
Cell Signaling Technology Smad2 antibody (Cell Signalling, 3108) was used in immunohistochemistry - paraffin section on human samples and in western blot on human samples at 1:1000. Cell Death Dis (2014) ncbi
rabbit monoclonal (138D4)
  • WB; pig
Cell Signaling Technology Smad2 antibody (Cell Signaling Technology, 3108) was used in western blot on pig samples. FASEB J (2014) ncbi
rabbit polyclonal
  • WB; mouse
Cell Signaling Technology Smad2 antibody (Cell Signaling Technology, 5678S) was used in western blot on mouse samples. Cell Death Dis (2014) ncbi
rabbit polyclonal
  • WB; rat
Cell Signaling Technology Smad2 antibody (Cell Signaling Technology, 3101) was used in western blot on rat samples. Int J Mol Med (2014) ncbi
rabbit polyclonal
  • IHC; zebrafish
Cell Signaling Technology Smad2 antibody (Cell Signalling, 3101S) was used in immunohistochemistry on zebrafish samples. PLoS ONE (2014) ncbi
rabbit monoclonal (D7G7)
  • WB; mouse; fig 13
Cell Signaling Technology Smad2 antibody (Cell Signaling Technology, 8685) was used in western blot on mouse samples (fig 13). BMC Nephrol (2014) ncbi
rabbit polyclonal
  • WB; rat
Cell Signaling Technology Smad2 antibody (Cell Signaling, 3101) was used in western blot on rat samples. Ann Neurol (2014) ncbi
rabbit monoclonal (138D4)
  • WB; human
Cell Signaling Technology Smad2 antibody (Cell Signaling, 3108) was used in western blot on human samples. Mol Biol Cell (2014) ncbi
rabbit polyclonal
  • WB; rat
In order to study the effect of rosiglitazone treatment on the transforming growth factor-beta/SMAD signaling pathway in white adipose tissue of diabetic rats, Cell Signaling Technology Smad2 antibody (Cell Signaling, 3101) was used in western blot on rat samples. Obesity (Silver Spring) (2014) ncbi
rabbit polyclonal
  • WB; rat; 1:1000
Cell Signaling Technology Smad2 antibody (Cell Signaling, 3104) was used in western blot on rat samples at 1:1000. Biochim Biophys Acta (2014) ncbi
rabbit polyclonal
  • WB; rat; 1:1000
Cell Signaling Technology Smad2 antibody (Cell Signaling, 3101) was used in western blot on rat samples at 1:1000. Biochim Biophys Acta (2014) ncbi
rabbit monoclonal (86F7)
  • WB; human; 1:1000
Cell Signaling Technology Smad2 antibody (Cell Signaling Technology, 86F7) was used in western blot on human samples at 1:1000. Br J Cancer (2014) ncbi
rabbit monoclonal (D7G7)
  • WB; human
Cell Signaling Technology Smad2 antibody (Cell Signaling Technology, 8685) was used in western blot on human samples. J Biol Chem (2014) ncbi
mouse monoclonal (L16D3)
  • WB; human; fig 1
Cell Signaling Technology Smad2 antibody (Cell Signaling Technology , 3103) was used in western blot on human samples (fig 1). FASEB J (2014) ncbi
rabbit polyclonal
  • WB; human
Cell Signaling Technology Smad2 antibody (Cell Signaling Technology, 3101S) was used in western blot on human samples. Cell Signal (2014) ncbi
rabbit polyclonal
  • WB; human; 1:1000
Cell Signaling Technology Smad2 antibody (Cell Signaling, 3101) was used in western blot on human samples at 1:1000. FASEB J (2014) ncbi
rabbit monoclonal (D43B4)
  • WB; human
  • IC; human; 1:50
Cell Signaling Technology Smad2 antibody (Cell Signaling Technology, D43B4) was used in western blot on human samples and in immunocytochemistry on human samples at 1:50. PLoS ONE (2013) ncbi
rabbit monoclonal (138D4)
  • WB; mouse; 1:1000; fig 7
In order to study the role of mitochochondrial Nox4 upregulation in the induction of cultured murine podicyte apoptosis by TGF-beta, Cell Signaling Technology Smad2 antibody (Cell Signaling Technology, 3108) was used in western blot on mouse samples at 1:1000 (fig 7). Am J Physiol Renal Physiol (2014) ncbi
rabbit monoclonal (D43B4)
  • WB; rat; 1:1000
Cell Signaling Technology Smad2 antibody (Cell Signaling, 5339S) was used in western blot on rat samples at 1:1000. J Neurochem (2014) ncbi
rabbit polyclonal
  • WB; human
Cell Signaling Technology Smad2 antibody (Cell Signaling, 3101) was used in western blot on human samples. Respir Res (2013) ncbi
rabbit polyclonal
  • WB; human
Cell Signaling Technology Smad2 antibody (Cell Signaling Technologies, 3104) was used in western blot on human samples. PLoS ONE (2013) ncbi
rabbit polyclonal
  • WB; human; 1:1000
Cell Signaling Technology Smad2 antibody (Cell Signaling, 3101) was used in western blot on human samples at 1:1000. Nucleic Acids Res (2013) ncbi
mouse monoclonal (L16D3)
  • WB; human; 1:2000
Cell Signaling Technology Smad2 antibody (Cell Signaling Technology, L16D3) was used in western blot on human samples at 1:2000. PLoS ONE (2013) ncbi
rabbit monoclonal (D43B4)
  • WB; mouse; 1:1000
Cell Signaling Technology Smad2 antibody (Cell Signaling, 5339) was used in western blot on mouse samples at 1:1000. PLoS ONE (2013) ncbi
rabbit monoclonal (138D4)
  • WB; mouse; 1:1000
Cell Signaling Technology Smad2 antibody (Cell Signaling, 3108) was used in western blot on mouse samples at 1:1000. PLoS ONE (2013) ncbi
rabbit polyclonal
  • WB; human
Cell Signaling Technology Smad2 antibody (Cell Signaling, 3101) was used in western blot on human samples. PLoS ONE (2013) ncbi
rabbit monoclonal (D43B4)
  • ChIP; human
Cell Signaling Technology Smad2 antibody (Cell Signaling, 5339) was used in chromatin immunoprecipitation on human samples. J Biol Chem (2013) ncbi
rabbit monoclonal (D43B4)
  • WB; rat
Cell Signaling Technology Smad2 antibody (CST, 5339) was used in western blot on rat samples. Diabetes (2013) ncbi
rabbit polyclonal
  • WB; rat
Cell Signaling Technology Smad2 antibody (CST, 3104) was used in western blot on rat samples. Diabetes (2013) ncbi
rabbit monoclonal (138D4)
  • WB; human
In order to examine the relation of GATA3 and TGF beta in breast cancer cells, Cell Signaling Technology Smad2 antibody (Cell Signaling Technology, 3108) was used in western blot on human samples. PLoS ONE (2013) ncbi
rabbit monoclonal (D7G7)
  • IP; rat
  • WB; rat
Cell Signaling Technology Smad2 antibody (Cell Signaling, 8685) was used in immunoprecipitation on rat samples and in western blot on rat samples. Am J Physiol Renal Physiol (2013) ncbi
rabbit monoclonal (138D4)
  • WB; chicken; 1:1000
Cell Signaling Technology Smad2 antibody (Cell Signaling, 138D4) was used in western blot on chicken samples at 1:1000. Gene (2013) ncbi
rabbit polyclonal
  • WB; human; 1:1,000
Cell Signaling Technology Smad2 antibody (Cell Signaling, 3101) was used in western blot on human samples at 1:1,000. PLoS ONE (2012) ncbi
rabbit polyclonal
  • WB; Domestic guinea pig
Cell Signaling Technology Smad2 antibody (Cell Signaling, 3101) was used in western blot on Domestic guinea pig samples. J Thorac Cardiovasc Surg (2013) ncbi
rabbit monoclonal (D43B4)
  • WB; Domestic guinea pig
Cell Signaling Technology Smad2 antibody (Cell Signaling, 5339) was used in western blot on Domestic guinea pig samples. J Thorac Cardiovasc Surg (2013) ncbi
rabbit polyclonal
  • IC; mouse; 1:300
  • WB; mouse; 1:1000
Cell Signaling Technology Smad2 antibody (Cell Signaling, 3101) was used in immunocytochemistry on mouse samples at 1:300 and in western blot on mouse samples at 1:1000. Development (2012) ncbi
rabbit monoclonal (138D4)
  • WB; human
  • WB; mouse
  • WB; rat
Cell Signaling Technology Smad2 antibody (Cell, 3108) was used in western blot on human samples, on mouse samples, and on rat samples. Am J Pathol (2012) ncbi
rabbit monoclonal (138D4)
  • WB; mouse; 1:1000
In order to study the role of Smad3 in the effect of TGF-beta in the mammary epithelium, Cell Signaling Technology Smad2 antibody (Cell Signaling, 3108S) was used in western blot on mouse samples at 1:1000. Mol Cancer Res (2012) ncbi
rabbit monoclonal (138D4)
  • WB; mouse; 1:500
In order to study the role of TGF-beta inhibitors in abolishing the resistance of glioblastoma to ionozing radiation therapy, Cell Signaling Technology Smad2 antibody (Cell Signaling, 3108) was used in western blot on mouse samples at 1:500. Cancer Res (2012) ncbi
not indicated
  • WB; human
Cell Signaling anti-Smad2 was used to perform western blot in order to study the roles of RBBP9 as a tumor-associated serine hydrolase to pancreatic neoplasiaProc Natl Acad Sci U S A (2010) ncbi
not indicated
  • WB; human
Cell Signaling Technology rabbit polyclonal Smad2 antibody was used to perform westn blot in order to investigate the relations between TGFbeta1 and TNFa.Mol Biol Cell (2009) ncbi
not indicated
  • WB; human
Cell Signaling Technology anti-phosph-Smad2 antibody was used to carry out western blot analysis in order to investigate the role for Smurf2 in promoting breast cancer metastasis.Cancer Res (2009) ncbi
not indicated
  • IHC; human
Cell Signaling phospho-Smad2 antibody was used in immunohistochemistry in order to investigate the role of PPAR-gamma in the regulation of bleomycin induced scleroderma.Am J Pathol (2009) ncbi
not indicated
  • WB; human
Cell Signaling Technology rabbit anti-phospho Smad2 antibody was used for western blot in order to investigate the role of ALK5 in the regulation of pulmonary arterial hypertension.Am J Pathol (2009) ncbi
not indicated
  • WB; human
Cell Signaling anti-phosphorylated Smad-2 antibody (no. 3101) was used in western blot to test a hypothesis that activin C antagonizes the effects of activin A and its overexpression leads to pathologies.Am J Pathol (2009) ncbi
not indicated
  • WB; human
Cell Signaling rabbit monoclonal anti-pSmad2 was used to perform western blot in order to show the mechanism that two functions of TGF become un-coupled during cancer development.Cancer Res (2008) ncbi
not indicated
  • WB; human
Cell Signaling mouse monoclonal anti-Smad2 was used to perform western blot in order to show the mechanism that two functions of TGF become un-coupled during cancer development.Cancer Res (2008) ncbi
not indicated
  • WB; human
Cell Signaling Smad2 antibody was used in western blot to study the role of Alk2 in activin signaling.J Endocrinol (2007) ncbi
not indicated
  • WB; human
Cell Signaling Technology anti-phospho-Smad2 and anti-Smad2 antibody was used in western blot to study the role of acetylation, one of the main post-translational regulatory mechanisms, in regulating Fli1 activity in human dermal fibroblast.J Biol Chem (2007) ncbi
not indicated
  • WB; human
Cell Signaling Technologies antiphospho-Smad2 antibody was used in western blot to study the role of disintegrin and metalloproteinase ADAM12 in TGF-beta signaling through interaction with the type II receptorJ Cell Biol (2007) ncbi
not indicated
  • IHC; human
Cell Signaling phospho-Smad2 antibody was used in immunohistochemistry to study the role of Alk3 during AV cushion morphogenesis in mouse embryonic hearts.Dev Biol (2007) ncbi
not indicated
  • IHC; human
Immunohistochemistry was performed using a polyclonal antibody recognizing pSmad2 (Cell Signaling Technology, Danvers).

Feedback from the author: Cell signaling ran out of this lot of pSmad2 antibody. Their current lot no longer works for IHC in mouse in our hands.

Science (2006) ncbi
not indicated
  • WB; human
Phospho-Smad2 antibody was used in western blot to study phospholation of Smad2 by caALK5J Biol Chem (2005) ncbi
BD Biosciences
mouse monoclonal (18/Smad2/3)
  • WB; mouse; 1:350
BD Biosciences Smad2 antibody (BD Biosciences, 610842) was used in western blot on mouse samples at 1:350. Reprod Sci (2016) ncbi
mouse monoclonal (18/Smad2/3)
  • WB; human; 1:1000
  • IC; human; 1:500
In order to show that tamoxifen prevents myofibroblast differentiation, BD Biosciences Smad2 antibody (BD Biosciences, 610842) was used in western blot on human samples at 1:1000 and in immunocytochemistry on human samples at 1:500. J Cell Physiol (2015) ncbi
mouse monoclonal (18/Smad2/3)
  • WB; human; fig 2
BD Biosciences Smad2 antibody (BD Biosciences, 610842) was used in western blot on human samples (fig 2). J Biol Chem (2015) ncbi
mouse monoclonal (18/Smad2/3)
  • WB; human
  • WB; mouse
BD Biosciences Smad2 antibody (BD Biosciences, 610842) was used in western blot on human samples and on mouse samples. J Biol Chem (2013) ncbi
mouse monoclonal (18/Smad2/3)
  • WB; mouse
  • WB; rat
BD Biosciences Smad2 antibody (BD, 610842) was used in western blot on mouse samples and on rat samples. Am J Pathol (2012) ncbi
mouse monoclonal (18/Smad2/3)
  • WB; mouse; 1:500
In order to study the role of TGF-beta inhibitors in abolishing the resistance of glioblastoma to ionozing radiation therapy, BD Biosciences Smad2 antibody (BD Transduction Laboratories, 610842) was used in western blot on mouse samples at 1:500. Cancer Res (2012) ncbi
not indicated
  • WB; human
BD Biosciences Smad2 monoclonal antibody was used in western blot in order to investigate the role of BMP9 signalling via ALK2/Smad1/Smad4 pathway in ovarian cancer.Cancer Res (2009) ncbi
not indicated
  • IHC; human
BD mouse monoclonal antibody against human and mouse Smad2 was used in immunohistochemistry to investigate the role of transforming growth factor Beta signaling in the regulation of tumor growth.J Natl Cancer Inst (2009) ncbi
not indicated
  • WB; human
BD Biosciences anti-Smad2/3 antibody was used to carry out western blot analysis in order to investigate the role for Smurf2 in promoting breast cancer metastasis.Cancer Res (2009) ncbi
not indicated
  • ChIP; human
BD Biosciences mouse anti-Smad2/3 antibody was used in ChIP assay to study the roles for ETS1 and TFAP2A in the transcription induced by TGF-beta-Smad pathways.Mol Cell Biol (2009) ncbi
not indicated
  • WB; human
BD Transduction Laboratories anti-Smad2/3 antibody was used in western blot to study COLO-357 cell growth inhibition by TGF beta1J Biol Chem (2005) ncbi
Sigma-Aldrich
rabbit polyclonal
  • WB; mouse; fig 13
Sigma-Aldrich Smad2 antibody (Sigma, SAB4504208) was used in western blot on mouse samples (fig 13). BMC Nephrol (2014) ncbi
rabbit polyclonal
  • IC; rat
Sigma-Aldrich Smad2 antibody (Sigma, SAB4300251) was used in immunocytochemistry on rat samples. Carcinogenesis (2014) ncbi
not indicated
  • IHC; human
Sigma alpha-smooth muscle actin (clone 1A4) antibody was used in immunocytochemistry to indicate potential therapeutic use of PDGF-D or PDGFR inhibitors to block human renal cell carcinoma tumor progression.Cancer Res (2005) ncbi
Source BioScience
not indicated
  • IP; human
  • EMSA; human
Autogen Bioclear goat anti-human Smad2 antibody was used in western blot to study TGF-beta1 signalingJ Biol Chem (2005) ncbi
Articles Reviewed
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